PcoCas12a: A novel CRISPR enzyme from Prevotella copri enhancing TCR-T-cell tumor suppression.

Genome editing technologies have been widely utilized in cell engineering, demonstrating immense potential in cell and gene therapy. However, an optimal gene-editing enzyme for immune cell editing remains unidentified. In this study, we identified a novel gene editing enzyme, termed CRISPR/PcoCas12a, derived from Prevotella copri, which recognizes a 5'-YYN PAM sequence.

The development logic, scientific Connotation, and promotion path of rural eco-industries in China.

The rural industry is the foundation for promoting farmers' income and rural development, and adhering to the green development of the rural industry is the internal requirement for comprehensively promoting Rural Revitalization Strategy (RRS) in China. Firstly, the article explains the three aspects of China's rural eco-industries' development logic: the rural eco-industries have the historical inevitability of action under the Ecological Civilization (EC), academic rationality of development under the theory of urban-rural integration development, and strategic action under ecological poverty alleviation. Secondly, it establishes the six components of the Chinese rural eco-eco-industries' scientific connotation: one guiding concept, three theoretical foundations, three development carriers, two practical paths, a 3*3 development model, and two fundamental goals.

Mass Spectrometry for O-GlcNAcylation.

O-linked -N-acetylglucosamine modification (O-GlcNAcylation) at proteins with low-abundance expression level and species diversity, shows important roles in plenty of biological processes. O-GlcNAcylations with abnormal expression levels are associated with many diseases. Systematically profiling of O-GlcNAcylation at qualitative or quantitative level is vital for their function understanding.

Analysis of the relationship between P-glycoprotein and abamectin resistance in Tetranychus cinnabarinus (Boisduval).

Abamectin is an effective acaricide and widely used in the control of Tetranychus cinnabarinus. With the increase of control failures, it is however important to clarify the resistance mechanism to improve the control of this mite. P-glycoprotein (Pgp) is an ATP-dependent drug efflux pump for xenobiotic compounds and is involved in multidrug resistance.

Expression characteristics of two novel cytochrome P450 genes involved in fenpropathrin resistance in Tetranychus cinnabarinus (Boisduval).

The carmine spider mite, Tetranychus cinnabarinus, which is also considered as the red form of Tetranychus urticae, is one of the most serious mite pests on crops. It is capable of rapidly developing resistance to acaricides, and has caused difficulty in controlling. However, the resistance mechanism of this mite remains unclear at molecular level.