Single-cell profiling reveals the intratumor heterogeneity and immunosuppressive microenvironment in cervical adenocarcinoma.

Background: Cervical adenocarcinoma (ADC) is more aggressive compared to other types of cervical cancer (CC), such as squamous cell carcinoma (SCC). The tumor immune microenvironment (TIME) and tumor heterogeneity are recognized as pivotal factors in cancer progression and therapy. However, the disparities in TIME and heterogeneity between ADC and SCC are poorly understood.

Arid4a Suppresses Breast Tumor Metastasis by Enhancing MTSS1 Expression via mRNA Stability.

Background: Tumor metastasis is one of the main causes of death in cancer patients; however, the mechanism controlling metastasis is unclear. The posttranscriptional regulation of metastasis-related genes mediated by AT-rich interactive domain-containing protein 4A (Arid4a), an RNA-binding protein (RBP), has not been elucidated.

Methods: Bioinformatic analysis, qRT-PCR, immunohistochemistry, and immunoblotting were employed to determine the expression of Arid4a in breast tumor tissues and its association with the survival of cancer patients.

Manganese-based nanoenzymes: from catalytic chemistry to design principle and antitumor/antibacterial therapy.

Manganese (Mn)-based materials have been extensively investigated for a wide range of biomedical applications owing to their remarkable catalytic chemistry, magnetic resonance imaging (MRI) capacity, biodegradability, low toxicity, and good biosafety. In this review, we first elaborate on the catalytic principle of Mn-based nanoenzymes for antitumor and antibacterial therapy, followed by a comprehensive discussion of the interesting structural design engineering strategies used to achieve multi-dimensional Mn-based nanoarchitectures, such as zero-dimensional (0D) nanoparticles, 1D nanotubes, 2D nanosheets, 3D hollow porous Mn ball, and core-shell nanostructures. Moreover, the therapeutic applications of different Mn-based nanoenzymes, including manganese dioxide (MnO)-based nanoenzymes that can trigger catalytic reactions, Mn-doped metal nanoenzymes and Mn-coordinated nanoenzymes that promote hydroxyl/reactive oxygen species (ROS) generation, and MnO-based micro/nanorobots that can effectively penetrate tumor tissues, are critically reviewed.

A NAC transcription factor and a MADS-box protein antagonistically regulate sucrose accumulation in strawberry receptacles.

Sugar accumulation during fruit ripening is an essential physiological change that influences fruit quality. While NAC transcription factors are recognized for their role in modulating strawberry (Fragaria × ananassa) fruit ripening, their specific contributions to sugar accumulation have remained largely unexplored. This study identified FvNAC073, a NAC transcription factor, as a key regulator that not only exhibits a gradual increase in gene expression during fruit ripening but also enhances the accumulation of sucrose.

Synergistic Effects of a Novel Multifunctional Bionic Scaffold and Electrical Stimulation Promote Bone Tissue Regeneration.

Electrical stimulation (ES) can effectively regulate cell behavior and promote bone tissue regeneration, and conductive biomaterials can further enhance this effect by enhancing the conduction of electrical signals between cells. In this study, poly(lactic-co-glycolic acid) (PLGA) and poly(l-lactide)-aniline pentamer triblock copolymer (PAP) were used as raw materials to prepare a conductive bionic scaffold (PLGA/PAP). Subsequently, bone morphogenetic protein 2 mimetic peptide containing a DOPA tag (DBMP2MP) was loaded on the scaffold surface.

Lyophilized monkeypox mRNA lipid nanoparticle vaccines with long-term stability and robust immune responses in mice.

The World Health Organization (WHO) has recently declared another global health emergency due to the rapidly spreading monkeypox (Mpox) outbreak in numerous African countries. To address the unmet need to contain the outbreak using the existing vaccines, this study developed a lyophilization process for an effective, scalable and affordable Mpox mRNA-LNP vaccine candidate to address the global health crisis. A comprehensive evaluation and optimization of the vaccine formulation (the type/concentration of cryoprotectants, the type/concentration of buffer system, as well as the mRNA concentration and reconstitution solvent) and the freeze-drying process parameters (freezing method, temperature, cooling rate and primary/secondary drying conditions) were conducted.

Superficial Temporal Artery-Middle Cerebral Artery Bypass Treatment Planning for Cerebral Ischaemia Based on Multi-Scale Model.

Superficial temporal artery and middle cerebral artery (STA-MCA) bypass surgery is an effective method to enhance cerebral blood flow (CBF) in ischemic patients. However, the effectiveness of various bypass techniques varies with the diversity of Circle of Willis (CoW) structures. This study aims to develop a physiologically realistic hemodynamic model to optimize STA-MCA bypass planning for cerebral ischemia patients with different CoW structures.

Revealing the Effects of Tympanic Membrane Implant Mechanical Properties on High-Frequency Hearing Loss After Clinical Myringoplasty: A Finite Element Analysis.

Myringoplasty is most commonly used to treat tympanic membrane (TM) perforation. Clinical data have shown that unexplained high-frequency (above 3 kHz) hearing loss often occurs after myringoplasty. In this paper, a finite element (FE) model of the partial external and middle ear (ME) of the human ear, which considers the actual perforation and TM implants, is developed to reveal the mechanical mechanism of high-frequency hearing loss after implantation of temporalis fascia and cartilage commonly used in myringoplasty.

Self-recoverable broadband near infrared mechanoluminescence from BaGaO:Cr using a multi-site occupation strategy.

Near infrared mechanoluminescence (NIR-ML) materials have attracted the attention of researchers due to their unique advantages, such as high resistance to bright-field interference and higher penetration depth into biological tissues. However, the reported NIR-ML materials are mainly rare-ion-activated narrow-band emitters. In this work, we report a NIR-ML material of BaGaO:Cr by a solid state reaction method.

Single -crystal sodium nickel phosphate nanoparticles as supercapacitor cathodes with ultra-high capacitance and rate-performance.

Sodium nickel phosphate (NaNiPO, NNP) is an attractive cathode material for high performance supercapacitors due to its abundance of active sites for oxidation/reduction, highly stable framework structure, . However, its disadvantages of low electric conductivity, disturbances of its impure crystalline phase, and the numerous pores/gaps produced by agglomerated polycrystalline morphologies in this cathode often limit its electrochemical performance. Herein, single-crystalline NNP rod-like nanoparticles with high phase purity have been prepared by spontaneous combustion combined with subsequent solid-phase calcination.

Fluorescent Sensor for the Visualization of Amino Acid Neurotransmitters in Neurons Based on an SAr Reaction.

Glutamate is an important excitatory neurotransmitter, while GABA is an inhibitory neurotransmitter. However, direct and accurate visualization of these important signaling agents by a chemical sensor is still very challenging. Here, a novel coumarin-based fluorescent sensor for the selective labeling and imaging of amino acids in neurons has been developed.

Pleural Fluid Amino Acids Contribute to Distinguishing Tuberculosis, Malignant, and Parapneumonic Pleural Effusion Patients.

Background: Differential diagnosis of pleural effusions poses a considerable challenge in clinical practice. In this study, we explored biomarkers in pleural fluid for distinguishing tuberculosis, malignant, and parapneumonic pleural effusion patients.

Methods: A total of 166 patients with exudative pleural effusion were collected, including 86 patients with tuberculosis pleural effusion (TPE), 52 patients with malignant pleural effusion (MPE), and 28 patients with parapneumonic effusion (PE).

Association of Multiple Clefts Nuclei in Blasts with TCF3::PBX1 Fusion in B-Lymphoblastic Leukemia/Lymphoma.

Background: Several particular kinds of typical morphology characteristics of leukemic blasts associated with the specific subtypes of leukemia have been reported. However, B acute lymphoblastic leukemia/lymphoma (B-ALL/LBL) has rarely been reported. The purpose of this study was to investigate the correlation of TCF3::PBX1 fusion with multiple clefts nuclei of blasts in patients with B-ALL/LBL.

Correlations of Prealbumin, Procalcitonin, and Brain Natriuretic Peptide with Acute Respiratory Infection in Children.

Background: We aimed to investigate the correlations of prealbumin (PA), procalcitonin (PCT) and brain natriuretic peptide (BNP) with acute respiratory infection (ARI) in children.

Methods: A total of 120 children with ARI admitted from June 2021 through June 2023 were selected (an infection group) and divided into a bacteria group (n = 50) and a virus group (n = 70) according to the results of bacterial culture and serum test. Another 90 healthy children who underwent physical examination in the same period were selected as a control group.

Modelling the effects of temperature, pH and osmotic shifts on the autofluorescence of in vitro.

Aims: This study aims to investigate how different wound microenvironmental factors (temperature, pH, and osmotic pressure) influence the autofluorescence of Staphylococcus aureus ( and its underlying molecular mechanisms, specifically focusing on the porphobilinogen synthase gene gene expression.

Methods: We measured the average fluorescence intensity of colonies under varying conditions of pH (3, 5, 7, 9, 11), temperature (25°C, 31°C, 37°C, 43°C), and osmotic pressure (0.9%, 1.

Weakly solvated perfluorinated electrolyte for high-temperature sodium-layered oxide cathodes.

The stability of the electrode-electrolyte interface in layered oxides is enhanced by electrolyte design criteria. A weakly-solvated electrolyte containing ethyl trifluoroacetate solvents with perfluorinated functional groups can restrain electrolyte decomposition and structural degradation when subjected to heat attack, exhibiting superior cycling durability at 60 °C compared to other fluorinated electrolytes.

LAMP2-FLOT2 interaction enhances autophagosome-lysosome fusion to protect the septic heart in response to ILC2.

Cardiac dysfunction is a serious complication of sepsis-induced multiorgan failure in intensive care units and is characterized by an uncontrolled immune response to overwhelming infection. Type 2 innate lymphoid cells (ILC2s), as a part of the innate immune system, play a crucial role in the inflammatory process of heterogeneous cardiac disorders. However, the role of ILC2 in regulating sepsis-induced cardiac dysfunction and its underlying mechanism remain unknown.

Monochromatic Responsive HOF Heterostructures via VIA-Group-Based Framework Hybridization for Fully-Covert Photonic Barcode.

Luminescent responsive heterostructures with region-domained emission and integrated responsiveness exhibit great potential in information security, but always suffer from the direct exposure of fingerprint information at the initial state, making it easy to decode the hidden confidential information. Herein, the first monochromatic responsive hydrogen-bonded organic framework (HOF) heterostructures are reported based on VIA-group-based framework hybridization toward fully-covert photonic barcodes. Designed HOF blocks with different VIA-group elements are integrated via a configuration-assimilation-based assembly method to generate the intrinsic monochromatic HOF heterostructures.

A Function-Oriented Binder with Exceptional Interface Ion Transport and Impurity Tolerance for Hard Carbon Anode.

Hard carbon is the sole anode material employed in commercial sodium-ion batteries. However, its intrinsic defects and impurities will lead to battery failure, diminishing further development of sodium batteries in energy storage. Here, an acrylonitrile copolymer and poly(ethylene oxide) (LA/PEO) composite binder is developed to address these challenges in biomass-derived hard carbon.

FLOT1 Is a Novel Serum Biomarker of Ovarian Cancer Targeted by N6-methyladenosine Modification Inhibition.

Ovarian cancer (OC) is a deadly disease and lacks a precise marker for diagnosis worldwide. Our previous work has shown the overexpression of flotillin-1 (FLOT1) in OC tissue. To improve diagnostic sensitivity and accuracy, we evaluated the serum level of FLOT1 in OC patients and found that the serum concentration of FLOT1 as well as CA125 was significantly increased in patients with OC compared with healthy control (p < 0.

Unraveling Compressive Strain and Oxygen Vacancy Effect of Iridium Oxide for Proton-Exchange Membrane Water Electrolyzers.

Iridium-based electrocatalysts are commonly regarded as the sole stable operating acidic oxygen evolution reaction (OER) catalysts in proton-exchange membrane water electrolysis (PEMWE), but the linear scaling relationship (LSR) of multiple reaction intermediates binding inhibits the enhancement of its activity. Herein, the compressive strain and oxygen vacancy effect exists in iridium dioxide (IrO)-based catalyst by a doping engineering strategy for efficient acidic OER activity. In situ synchrotron characterizations elucidate that compressive strain can enhance Ir─O covalency and reduce the Ir─Ir bond distance, and oxygen vacancy (O) as an electronic regulator causes rapid adsorption of water molecules on the Ir and adjacent Ov (Ir─O) pair site to be coupled directly into O─O intermediates.

Dual Type-II Colloidal Quantum Wells for Efficient Nonlinear Optical Limiting.

Colloidal II-VI nanocrystals have garnered significant research attention in nonlinear optical applications due to their low-cost synthesis, photophysical tunability, and ease of device integration. Herein, we report that dual-type II CdSe/CdTe/CdSe colloidal quantum wells (CQWs) with core/crown/crown structures achieve remarkable nonlinear optical limiting capabilities driven by an exceptionally large nonlinear absorption coefficient. Open aperture -scan reveals that these dual-type II CQWs exhibit a third-order nonlinear absorption coefficient of 33.

Co-Delivery of Morphologically Switchable Au Nanowire and Hemoglobin-Resveratrol Nanoparticles in the Microneedle for Diabetic Wound Healing Therapy.

Diabetic wounds are a common complication of diabetes and pose a significant threat to human health. High glucose concentration in the wound remains a major obstacle, necessitating effective strategies to achieve sustained glucose consumption for synergistic diabetic wound therapy. In this study, an Au-based nanomaterial is developed that can adjust its morphology in different therapeutic processes.

Galvanic Cell Bipolar Microneedle Patches for Reversing Photoaging Wrinkles.

Excessive exposure to ultraviolet (UV) radiation is a major factor in the development of skin photoaging wrinkles. While current treatments can slow the progression of photoaging, it is very difficult to achieve complete reversal. This study introduces galvanic cell microneedle (GCMN) patches with magnesium-containing bipolar electrodes.