Publications by authors named "JianWen Teng"

Liupao tea (LPT) is a Chinese dark tea known to possess a unique flavour. Microbial fermentation plays a crucial role in flavour development and enrichment. Currently, the phytochemical profiles and bioactivities of LPT with and without fermentation are not fully known.

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  • * Researchers analyzed six samples of Liupao tea using metabolomics and sensory evaluation, identifying 477 non-volatile compounds, including 18 potential bitter and 22 potential astringent compounds.
  • * Key compounds linked to bitterness (like caffeine and catechin gallate) and astringency (like gallic acid) varied significantly during fermentation, affecting the tea's flavor profile and offering insights for quality enhancement.
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Background: The adhesion of probiotics to the intestine is crucial for their probiotic function. In previous studies, Tremella polysaccharides (TPS) (with sodium casein) have shown the potential to encapsulate probiotics and protect them in a simulated gastrointestinal tract. This study explored the effect of TPS (with sodium casein) on the adhesion of probiotics.

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The increase of polysaccharides in the dark tea pile process is thought to be connected to the cell wall polysaccharides' breakdown. However, the relationship between tea polysaccharides (TPSs) and tea cell wall polysaccharides has not been further explored. In this study, the structural changes in the cell wall polysaccharides [e.

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Background: Pile fermentation is one of the key steps in developing the Liupao tea (LBT) quality and unique characteristics. The complex biochemical profile of LBT results from microorganisms present during the pile-fermentation process. However, the critical underlying microorganisms and the marker compounds still need to be determined.

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  • Tank fermentation of Liupao tea involves a diverse microbial community, with major genera including Sphingomonas, Aquabacterium, and Aspergillus, which differ from those found in pile fermentation.
  • Fifteen genera were identified as biomarkers, with functional analyses indicating a focus on metabolic processes for carbohydrates and amino acids.
  • The study isolated 283 strains representing various bacteria and fungi, highlighting beneficial microbes that could enhance the fermentation process and supporting advancements in dark tea production technologies.
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  • A natural polysaccharide named TPS-5 was extracted from Liupao tea, with a molecular weight of 48.289 kDa and characterized as a pectin-type acidic polysaccharide.
  • The molecular structure of TPS-5 features a specific backbone and branches, contributing to its unique properties.
  • Biological evaluations showed that TPS-5 exhibits various health benefits, including antioxidant and digestive properties, indicating its potential use in functional foods or medicinal applications.
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Though rice proteins have been applied to improve the stability of phenolic compounds, it is unclear how rice proteins affect phenolic acid's digestion and bioavailability. This study investigated the consequences of protein-ferulic acid interactions in the gastrointestinal environment. Ferulic acid and rice proteins formed complexes at room temperature, both with and without laccase.

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Mango is one of the most economically important fruit; however, the gene regulatory mechanism associated with ripening and quality changes during storage remains largely unclear. This study explored the relationship between transcriptome changes and postharvest mango quality. Fruit quality patterns and volatile components were obtained using headspace gas chromatography and ion-mobility spectrometry (HS-GC-IMS).

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Liupao tea is a dark tea with unique quality. Semi-finished Liupao tea with two different fermentation processes (traditional/tank) was analyzed to explain the chemical characteristics and taste quality. The content change rate of polyphenols, flavonoids, and theabrownin in traditional fermentation was approximately twice that in tank fermentation.

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Background: The targeted biological activity of a natural product is often the result of the combined action of multiple functional components. Screening for predominant contributing components of targeting activity is crucial for quality evaluation.

Results: Thirteen and nine phenolic compounds inhibiting α-glucosidase and α-amylase, respectively, were identified in the ethanol extracts of passion fruit peel through liquid chromatography-tandem mass spectrometry and multivariate analysis.

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Liupao tea is an important dark tea, but few studies on purified Liupao tea polysaccharide (TPS) are reported in the literature. In this study, two TPSs, named TPS2 and TPS5, with molecular weights of 70.5 and 133.

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  • Liupao tea, like Pu-erh tea, undergoes natural fermentation involving the microorganism Penicillium citrinum, which can produce citrinin, a toxic compound.
  • Research analyzed how P. citrinum affects the nutritional content of Liupao tea, revealing significant decreases in key components like sugars, pectins, amino acids, and polyphenols as its growth occurs.
  • Findings indicate that specific biosynthesis genes (ctnA and orf5) are crucial regulators of citrinin production, suggesting they could be targets for controlling both the fungus and the associated toxic effects in Liupao tea.
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  • Liupao tea may be more effective than green tea in reducing cholesterol and improving antioxidant enzyme levels for those with hyperlipidemia.
  • Both teas were found to decrease total cholesterol and triglycerides, while Liupao tea showed a significantly greater reduction in LDL-C and an increase in antioxidant activity.
  • A total of 93 compounds were identified in the teas, with Liupao tea containing higher levels of certain active substances that correlate with its beneficial effects.
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  • Penicillium citrinum, found in dark tea like Liupao, can produce a harmful kidney toxin called citrinin (CIT), making its detection challenging due to complex tea composition and diverse fungi.
  • A new real-time PCR (qPCR) method was developed to detect specific genes related to CIT production and assess the toxin levels at various production stages of Liupao tea.
  • The study found CIT present during the pile-fermentation process and in some aged samples, and the qPCR method showed high sensitivity in predicting CIT production and identifying potential toxin-producing fungi.
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Fermentation often degrades the cell wall of dark tea, changes the carbohydrate components in the cell wall of tea, and thus affects the quality of tea. However, the lack of ultrastructural details limits our knowledge on the effect of fermentation on tea cell walls. Morphological studies of cell structures are important; thus, the cell wall of Liupao tea was analyzed under transmission electron microscopy for the first time, and the effects of different raw materials and fermentation methods on the cell wall and main carbohydrates of tea were compared.

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Passion fruit peel, a potential source of bioactive compounds, has been used as food stabilizing agent. However, the phenolic composition and bioactivity of passion fruit peel have rarely been reported. The effects of simulated gastrointestinal digestion on the bioactive components, bioactivity and bioaccessibility of passion fruit peel ethanol extracts (PFPE) were investigated using high performance liquid chromatography-tandem mass spectrometry analysis (quasi-targeted metabolomics).

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  • The study extracted and purified raw and aged polysaccharides from Liupao tea, resulting in five refined fractions.
  • The analysis showed that the polysaccharide content increased in aged tea while the molecular weight decreased due to fermentation.
  • Aged tea polysaccharides (ALTPS) demonstrated better thermal stability and health benefits, including improved anticoagulant and bile acid binding activities compared to raw tea polysaccharides (RLTPS).
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Citrinin (CIT), a known nephrotoxic mycotoxin, is mainly produced by , , and species. It is a natural contaminant in cereal grains, foods, and feedstuff. Liupao tea (or Liubao tea) is a typical Chinese dark tea obtained via indigenous tea fermentation facilitated by microorganisms.

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  • Liupao tea is a unique Chinese dark tea produced through natural fermentation involving bacteria and fungi.
  • MiSeq sequencing revealed a diverse fungal community with 85 strains across four samples, highlighting Eurotium and Aspergillus as the main fungal genera.
  • The study is significant for identifying new Eurotium species associated with Liupao tea and lays the groundwork for further research on its fermentation processes and microbial functions.
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  • Researchers studied extracts from Blumea riparia DC. to assess their impact on blood clotting and hemostasis in mice.
  • The ethyl acetate (EtOAc) fraction notably decreased both blood clotting time and tail bleeding time, indicating enhanced coagulation.
  • Key phenolic acids (vanillic, p-coumaric, and protocatechuic) demonstrated procoagulant effects and improved uterine contractions, suggesting they work through different mechanisms to promote blood coagulation.
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