Epitympanoplasty with cartilage obliteration in the preservation of posterior canal wall: a technique for surgical treatment of attic cholesteatoma.

To report and evaluate the surgical outcomes of the treatment of attic cholesteatoma using epitympanoplasty with cartilage obliteration in the preservation of the posterior canal wall. Our study group consists of 138 subjects with attic cholesteatoma who underwent surgery of the mastoidectomy of the intact posterior canal wall and epitympanoplasty with cartilage obliteration, from November 2008 to November 2010. The major techniques employed included the following: (1) mastoidectomy with the preservation of the posterior canal; (2) removal of the scutum; (3) epitympanum obliteration with cartilage, and (4) mastoid obliteration with bone dust.

Study on the electrochemical catalytic properties of the topological insulator Bi₂Se₃.

In this work, the electrochemical catalytic properties of the topological insulator bismuth selenide (Bi₂Se₃) were first studied. In the presence of Bi₂Se₃ the reduction current of dissolved O₂ could be significantly enhanced. The electron transfer resistivity (Rct) was greatly reduced at the Bi₂Se₃-PVP modified electrode as evidenced by the electrochemical impedance spectrometry, implying that the topological insulator Bi₂Se₃ could facilitate the electron transfer at the interface due to the excellent surface conductivity.

[Comparative study of determining the sites of airway obstruction in obstructive sleep apnea hypopnea syndrome between real-time CT scans and laryngofiberscope technology].

Objective: To compare 256-layer spiral computed tomography (CT) scan in sleep and laryngofiberscope technology for locating obstructive sites of upper airway in patients with obstructive sleep apnea hypopnea syndrome, analyze their advantages and disadvantages and discuss the clinical application values.

Methods: A total of 59 patients with OSAHS diagnosed by polysomnography underwent spiral CT scan in awake and drug-induced sleep states and laryngofiberscope examination in awake state to assess the sites of airway obstruction.

Results: Real-time CT scans were completed successfully in all patients.

[Measurement of objective parameters associated with pharyngeal swallowing function in Chinese adults].

Objective: To obtain a series of objective criteria associated with pharyngeal swallowing function using dynamic swallow study in Chinese adults.

Methods: Dynamic videofluoroscopic swallow studies were performed on 80 normal adult volunteers. There were 40 males and 40 females aged from 20 to 60 years old.

[Study of the relationship between sleep body posture, sleep phase and severity of obstructive sleep apnea-hypopnea syndrome].

Objective: To study the clinical characteristics and relationship between sleep body posture, sleep phase and occurrence in patients with various degree of obstructive sleep apnea-hypopnea syndrome (OSAHS).

Method: Polysomnography recordings of 100 adults with OSAHS were divided into 3 groups according to AHI: mild with apnea-hypopnea indices (5 < or = AHI < or = 15), moderate (15 < AHI < or = 30) and severe (30 < AHI). The polysomnography data and clinical characteristics were compared between each groups.

[Roles of cardiovascular disease in obstructive sleep apnea hypopnea syndrome].

Objective: To explore the roles of cardiovascular disease in obstructive sleep apnea hypopnea syndrome.

Method: All 228 OSAHS patients underwent the examination of biochemical and complete blood count. The correlation analysis between AHI, AI, HI, LSaO2, complete blood count, and biochemical examination were employed.

Dopamine D1, D2, D3 receptors, vesicular monoamine transporter type-2 (VMAT2) and dopamine transporter (DAT) densities in aged human brain.

The dopamine D(1), D(2), D(3) receptors, vesicular monoamine transporter type-2 (VMAT2), and dopamine transporter (DAT) densities were measured in 11 aged human brains (aged 77-107.8, mean: 91 years) by quantitative autoradiography. The density of D(1) receptors, VMAT2, and DAT was measured using [(3)H]SCH23390, [(3)H]dihydrotetrabenazine, and [(3)H]WIN35428, respectively.

Hot electron-induced cathodic electrochemiluminescence at oil film-covered carbon paste electrode and application to nano-molar determination of catechol.

Hot electron-induced cathodic electrochemiluminescence of the Ru(bpy)(3)(2+)/S(2)O(8)(2-) system was investigated at an oil film-covered carbon paste electrode (CPE) under cathodic pulse polarization for the first time. Compared with other electrodes, the CPE is of lower background, better stability and reproducibility. The method is also applied to the determination of catechol.

Mycobacterium tuberculosis ESAT-6 exhibits a unique membrane-interacting activity that is not found in its ortholog from non-pathogenic Mycobacterium smegmatis.

Mycobacterium tuberculosis ESAT-6 (MtbESAT-6) reportedly shows membrane/cell-lysis activity, and recently its biological roles in pathogenesis have been implicated in rupture of the phagosomes for bacterial cytosolic translocation. However, molecular mechanism of MtbESAT-6-mediated membrane interaction, particularly in relation with its biological functions in pathogenesis, is poorly understood. In this study, we investigated the pH-dependent membrane interaction of MtbESAT-6, MtbCFP-10, and the MtbESAT-6/CFP-10 heterodimer, by using liposomal model membranes that mimic phagosomal compartments.

[Habituation of seasickness in adult during a long voyage].

Objective: To observe the habituation of seasickness in non marine subjects during a long voyage.

Methods: A crew of 106 staffs of the Hospital Ship Ark Peace was included in this study. There were 59 male and 47 female with an age ranged from 23 to 53 years (mean 37.

Effects of carbogen on cochlear blood flow and hearing function following acute acoustic trauma in guinea pigs.

Background And Aims: Disturbances of microcirculation and hemorheological changes in the inner ear are the results of noise-induced hearing loss (NIHL). Both the disturbances of microcirculation and hemorheological changes are the etiologies of NIHL development, but they are also the results. Although previous reports that inhalation of high concentration of CO(2) may increase cochlear blood flow (CoBF), the effects of carbogen on the cochlear microcirculation and NIHL remain unclear.

New model of in-situ xenograft lymphangiogenesis by a human colonic adenocarcinoma cell line in nude mice.

Objective: To explore a new model of in-situ xenograft lymphangiogenesis of human colonic adenocarcinomas in nude mice.

Method: On the basis of establishing subcutaneous xenograft lymphangiogenesis model of human colonic adenocarcinoms, in-situ xenografts were established through the in situ growth of the HT-29 human colonic adenocarcinoma cell line in nude mice. The numbers of lymphangiogenic microvessels, the expression of lymphatic endothelial cell markers lymphatic vessel endothelial hyaloronic acid receptor-1 (LYVE-1), D2-40 and the lymphatic endothelial growth factors vascular endothelial growth factor-C (VEGF-C), -D (VEGF-D) and receptor-3 (VEGFR-3) were compared by immunohistochemical staining, Western bolt and quantitative RT-PCR in xenograft in-situ models.

[Tissue engineered tympanic membrane repairment materials with adipose-derived stem cells].

Objective: To explore the morphology, proliferative activity and adhesion of adipose-derived stem cells (ASC) seeded in the polylactic-co-glycolic acid (PLGA) scaffold, and to provide experimental data for fabricating tissue engineered tympanic membrane repairment materials.

Methods: Wistar rats were selected, and the ASC were isolated and co-cultured with the PLGA scaffold. The morphology and proliferative activity of ASC in the scaffold were examined by immunofluorescence of Vimentin and Ki67 respectively.

Suppression of lymphangiogenesis in human lymphatic endothelial cells by simultaneously blocking VEGF-C and VEGF-D/VEGFR-3 with norcantharidin.

Lymph node metastasis of tumors is a crucial early step in the metastatic process. Tumor lymphangiogenesis plays an important role in promoting tumor metastasis to regional lymph nodes. Norcantharidin (NCTD) has been reported to possess potent anti-angiogenesis and antitumor properties in several cell lines and xenograft tumor models.

[Clinical analysis of the adolescent patients with multi-segments intramedullary spinal cord tumors].

Objective: To prospectively analyze the clinical features and characteristics of multi-segments intramedullary spinal cord tumors in adolescent patients.

Methods: In our study, 25 consecutive adolescent patients with multi-segments intramedullary spinal cord tumors were recruited, who underwent microsurgery for the tumor using a posterior approach and were hospitalized in Peking University Third Hospital within a period of 8 years. The tumor was exposed through dorsal myelotomy.

An experimental study on tympanic membrane reconstruction with acellular dermal matrix.

Conclusion: Acellular dermal matrix (ADM) can be used as a substitute for tympanic membrane (TM) reconstruction.

Objective: To investigate the effectiveness of ADM in the treatment of TM perforations.

Methods: Fifty guinea pigs were used to create TM perforations.

CD146 expression correlates with epithelial-mesenchymal transition markers and a poor prognosis in gastric cancer.

CD146 has been regarded as a novel potential therapeutic target for multiple cancers. The aim of the study was to investigate the expression of CD146 in gastric cancer and evaluate its clinical-pathological and prognostic significance. The expression of CD146 and three epithelial-mesenchymal transition (EMT)-related proteins (E-cadherin, β-catenin and vimentin) was examined in 144 gastric cancers by immunohistochemistry.

[Development of portable free hemoglobin detector].

The paper designed the portable free hemoglobin detector using spectrophotometry, which determines the concentration of free hemoglobin. The device has features of portability, compact and precise. It can evaluate the quality of blood products instantly and effectively.

[Dynamic imaging assessment on the upper airway in patients with obstructive sleep apnea hypopnea syndrome].

It is vital to make an individual plan for each patient with obstructive sleep apnea-hypopnea syndrome (OSAHS) according to the obstruction sites. The high resolution anatomical information of upper airway and soft tissue can be obtained, especially by MRI and CT scans. Dynamic and state-dependent imaging techniques are beneficial to study stereo changes of anatomy and morphology of upper airway in quiet breathing, sleeping or airway closure.

NR5A nuclear receptor Hr39 controls three-cell secretory unit formation in Drosophila female reproductive glands.

Background: Secretions within the adult female reproductive tract mediate sperm survival, storage, activation, and selection. Drosophila female reproductive gland secretory cells reside within the adult spermathecae and parovaria, but their development remains poorly characterized.

Results: With cell-lineage tracing, we found that precursor cells downregulate lozenge and divide stereotypically to generate three-cell secretory units during pupal development.

[Relationship between obstructive sleep apnea-hypopnea syndrome and hypertension].

Objective: To explore the relationship between obstructive sleep apnea-hypopnea syndrome (OSAHS) and hypertension.

Methods: There were 1149 middle aged patients who were suspected to have OSAHS underwent polysomnography (PSG) from Jan, 1999 to June, 2011. The age of the patients ranged from 45 to 65 years.

Rapid visual detection of aluminium ion using citrate capped gold nanoparticles.

We report the visual detection of Al(3+) using unlabeled gold nanoparticles (AuNPs) based on the complexation of Al(3+) with citric acid, resulting in the aggregation of AuNPs. The distinction of color change can be observed by the naked eye at concentrations down to 1.0 μM which is lower than the permissable level (7.

[Electronic laryngoscope for transnasal esophageal examination].

Objective: To evaluate the value of electronic laryngoscope for transnasal esophagoscopy.

Methods: The electronic laryngoscope was used for transnasal esophagoscopy in 50 patients from June 2009 to June 2011 in our department. There were 32 males and 18 females with their age ranged from 16-88 years (mean 53.

[Clinical observation of mucoregulatory agents' application after chronic rhinosinusitis surgery].

Objective: To explore the effect of mucoregulatory agents during endoscopic sinus surgery.

Method: Ninety-seven cases with chronic rhinosinusitis were randomly divided into three groups, with 31 cases in B group treated by ambroxol, 33 cases in C group treated by eucalyptol-limonene-pinene enteric soft capsule and 33 cases in control group (A group). The follow-up visit lasted for 6 months for three groups.

[Preliminary exploration of Gd-DTPA enhanced inner ear image of guinea pig using magnetic resonance scan].

Objective: To observe the distribution of Gd-DTPA in the inner ear of guinea pig by MRI at different time points after intratympanic administration, explore the optical time for observing the whole inner ear. To study the pharmacokinetic feature of Gd-DTPA in the inner ear, and find out whether discrimination of endolymph and perilymph can be obtained under current conditions.

Methods: Sixty-five guinea pigs were randomly divided into 13 groups, after diluted Gd-DTPA intratympanic injection, each group of guinea pigs were scanned through MRI (3D-T1 FSE sequence) at different time points (0 h, 0.