[Rapid diagnosis of Down's and Edward's syndrome by multiplex real-time quantitative PCR].

Objective: To establish a multiplex real-time quantitative PCR method for diagnosis of Down's and Edward's syndrome.

Methods: The sequences of the amyloid precursor protein gene (APP) in the Down's region of chromosome 21 and the thymidylate synthetase gene (TYMS) on chromosome 18 were co-amplified in the same tube. The relative quantitative index DeltaCT value was used to differentiate Down's and Edward's syndrome patient from healthy individual.