[Identification of Weak D Type 1 in Rh Blood Group System and Discussion of Transfusion Strategy].

Objective: To investigate the molecular mechanism of one patient with abnormal serological phenotype in RhD and discuss the transfusion strategy.

Methods: The RhD variant sample was screened from a patient with IgM type anti-D antibody and further determined by three different sources of anti-D antibodies. Ten exons and the adjacent introns of the RHD gene were amplified, purified and sequenced.

[Different Subtypes Caused by c.721C>T Substitution in the Exon 7 of ABO Gene].

Objective: To analyze the different subtypes caused by c.721C>T substitution in the exon 7 of the ABO gene, and to investigate the related molecular mechanism of different antigens expression.

Methods: ABO subtypes in 7 samples were identified by standard serological methods.

[Differences of drought tolerance of the main tree species in Dongling Mountain, Beijing, China as indicated by tree rings].

Forests in Beijing exhibit vulnerability to increasing stress of extreme drought in recent years. To investigate the drought tolerance of different tree species, we chose three tree species (, , and ) from the forest of Dongling Mountain in Beijing and used dendroecological method to analyze the relationship between radial growth and climate, as well as their resistance and resilience to extreme drought events. Our results showed that the radial growth of and was significantly negatively correlated with monthly mean temperature from May to June, but that of was significantly negatively correlated with monthly mean temperature only in May.

Bronchogenic cyst of the stomach: A case report.

Background: Gastric bronchogenic cysts (BCs) are extremely rare cystic masses caused by abnormal development of the respiratory system during the embryonic period. Gastric bronchial cysts are rare lesions first reported in 1956; as of 2019, only 37 cases are available in the MEDLINE/PubMed online databases. BCs usually have no clinical symptoms in the early stage, and their imaging findings also lack specificity.

[MAX-DOAS Tomography Reconstruction for Gas Plume].

In order to achieve precisely two-dimensional spatial distribution reconstruction of smoke plume, passive MAX-DOAS tomography is established, the measurement of the spatial distribution of the exhaust plume is implemented by more passive multi-axis differential absorption spectrum system. First, the multi-axis differential absorption spectrum system and its mechanism of inverse gas concentration are introduced in the paper. Then, algebra iterative algorithm is adopted to extract the information of the trace gas concentration in reconstruction simulation with different models and different scanning optical path, and the reconstruction program is designed.

[Polymorphisms of RHCE gene among serologic RhD negative donors of Han population in Chinese Jiangsu area].

This study was purposed to investigate the RHCE gene polymorphisms in Chinese Jiangsu Han blood donors with and without RHD gene among serologic RhD negative population. PCR with sequence-specific primers (PCR-SSP) was used to detect RHCE genotype in 337 serologic RhD negative, RHD gene positive donors. The RHD gene-specific polymorphisms were also determined by PCR-SSP in these donors.

Rare myeloid sarcoma/acute myeloid leukemia with adrenal mass after allogeneic mobilization peripheral blood stem cell transplantation.

Myeloid sarcoma (MS) is a rare hematological neoplasm that develops either de novo or concurrently with acute myeloid leukemia (AML). This neoplasm can also be an initial manifestation of relapse in a previously treated AML that is in remission. A 44-year-old male patient was diagnosed with testis MS in a local hospital in August 2010.

[Feasibility of volume perfusion CT (VPCT) imaging in antiangiogenic treatment of rabbit VX2 soft-tissue tumor].

Objective: To explore the feasibility of volume perfusion CT imaging to dynamically monitor and evaluate the response of rabbit VX2 soft-tissue tumor to antiangiogenic treatment.

Methods: To establish an experimental animal model of VX2 soft tissue tumor on 20 New Zealand white rabbits. Twenty rabbits were randomly divided into 2 groups.

[Analysis of cavitation of advanced NSCLC treated by rh-endostatin combined with NP chemotherapy].

Objective: To study the significance of intra-tumoral cavitation in the patients with advanced NSCLC treated by rh-endostatin plus NP chemotherapy.

Methods: Fifty-seven patients with advanced NSCLC were randomly assigned to receive chemotherapy with rh-endostatin plus NP or NP alone. The numbers of activated circulating endothelial cells (aCECs) were measured by flow cytometry.

[Effects of platelet-derived membrane microparticles on angiogenesis in chick chorioallantoic membranes].

This study was purposed to investigate the angiogenesis effect of platelet-derived membrane microparticles (PMPs) in chick chorioallantoic membranes (CAM). Thrombin were adopted to activate the platelets and then PMPs were obtained. PMPs were isolated by high speed centrifugation.

[Test of activated plasma clotting time to assess efficacy of platelet transfusion].

The study was aimed to investigate the value of activated plasma clotting time (APCT) for estimating the efficacy of platelet transfusion therapy. There were twenty patients with hematological diseases, who received transfusion of platelet, involved in the test. APCT was determined before and after transfusion of these patients, then APCT was contrasted with corresponding CCI and PPR.

[Cold storage of rabbit platelet suspension by adding uridine diphosphate galactose].

This study was aimed to investigate the method to cold-store platelets with uridine diphosphate galactose (UDP-Gal). Rabbit heart blood was prepared for concentrated platelet suspension to which UDP-Gal was added, and then stored for ten days in 4 degrees C refrigerator. Thereafter, platelet count, mean platelet volume (MPV), platelet distributing width (PDW), platelet aggregation function, platelet activity to urge coagulation including PF3aT and APCT and apoptosis were determined.

[Influence of raising oxygen content on function of platelet concentrate during preservation].

To explore the influence of raising oxygen (dissolved oxygen) content on function of platelet concentrate, the platelet concentrate was prepared by a CS-3000 plus blood cell separator. Experiments were divided into 2 groups: test group and control group. After raising oxygen content in platelet plasma under sterile operation, the platelet samples of two groups were preserved in oscillator with horizontal oscillation at 22 +/- 2 degrees C.

[Application of cationic propyl gallate as inducer of thrombocyte aggregation for evaluating the platelet function of platelet donors].

The purpose of study was to investigate the feasibility of the application of cationic propyl gallate (C-PG) as inducer of platelet aggregation for evaluating the platelet function of single-donor plateletpheresis and identifying the incidence of defective platelet function among donors. Experiments were as follows: 3 healthy volunteers' platelet aggregation induced by 100-300 micromol/L C-PG was determined by LG-PABER analyzer to observe the effect of C-PG concentration on platelet aggregation; 30 healthy volunteers' platelet aggregation before and 24 hours after administration of 200-400 mg acetylsalicylic acid (ASA) was examined after induction by 200 micromol/L C-PG for determining the cut-off value to discriminate platelet dysfunction donors; the platelet aggregation of 483 platelet donors was detected and the activated plasma clotting time (APCT) of donors who have deficiency in platelet aggregation was examined for investigating the incidence of defective platelet function among donors. The results showed that platelets were activated by C-PG induction in a dose dependent manner, when concentration of C-PG reached 200 micromol/L, the percentage of platelet aggregation was highest.

[Value of imaging in the diagnosis of primary malignant fibrous histiocytoma of bone].

Objective: To investigate the imaging feature of primary malignant fibrous histiocytoma of the bone (PBMFH) by the conventional radiography, CT and MRI, and to evaluate these different imaging methods in its diagnosis.

Methods: The imaging data of conventional radiography, CT and MRI of 35 patients with pathologically confirmed PBMFH were retrospectively analyzed.

Results: Though the imaging appearance of PBMFH varied in different cases, all the imaging findings of malignant bone tumors were revealed.