Combination of 308-nm excimer laser with topical pimecrolimus for the treatment of childhood vitiligo.

Forty-nine patients enrolled in a single-blinded, randomized, comparing 308-nm excimer laser therapy together with topical 1% pimecrolimus cream twice daily (group A) with excimer laser therapy twice per week (group B). Of 48 patients evaluated after 30 weeks of treatment, 71% of patients from group A achieved Grade 3 or 4 repigmentation compared with 50% in group B. Significant difference was found between group A and B at the end of 30 weeks of treatment (p = 0.

[Construction and expression of HSV-2gD-Hsp70 fusion protein gene].

To construct and express Hsp70-HSV2gD fusion protein. Genes of Hsp70 and HSV-2gD were subcloned into vectors pGEX-4T-1 respectively. After confirmed by DNA sequence analysis, the recombinant plasmids pGEX-4T-HSP-gD was transformed into E.

[Expression of anti-keratin human Fab in Pichia pastoris and optimization of expression condition].

Aim: To express human anti-keratin Fab in Pichia pastoris secretively and optimize the expression condition.

Methods: Genes of kappa chain and Fd fragment of anti-keratin antibody from the plasmids p3MH/Fab were subcloned into vectors pPIC9K and pPICZalphaA respectively. After confirmed by DNA sequence analysis, the recombinant plasmids pPIC9K/L and pPICZalphaA/Fd were transducted into the genome of GS115 Pichia pastoris using two-step integrating technology.

[Construction and expression of eukaryotic expression vector for intact IgG against human keratin].

Aim: To construct an eukaryotic expression vector containing the gene of anti-keratin antibody and express it in CHO(dhfr(-)) cells.

Methods: Both the V(L) and V(H) genes of an anti-keratin Fab from a phage display library were amplified by PCR. Using PCR product as the template, the V(kappa) and V(H) genes with the leader sequences (named L(kappa) and L(H) respectively) were amplified by overlapping PCR.

[Expression of human Fab antibody against keratin in E.coli and its renaturation].

Aim: To express Fd fragment and L chain of human anti-keratin Fab in E.coli BL21(DE3) respectively and obtain human anti-keratin Fab by renaturation in-vitro.

Methods: Genes of L chain and Fd fragment of anti-keratin antibody from the plasmids p3MH/ Fab were subcloned into vector pET32a respectively.