Thorectidiol A Isolated from the Marine Sponge Disrupts Interactions of the SARS-CoV-2 Spike Receptor Binding Domain with the Host ACE2 Receptor.

Thorectidiols isolated from the marine sponge (family Thorectidae, order Dictyoceratida) collected in Papua New Guinea are a family of symmetrical and unsymmetrical dimeric biphenyl meroterpenoid stereoisomers presumed to be products of oxidative phenol coupling of a co-occurring racemic monomer, thorectidol (). One member of the family, thorectidiol A (), has been isolated in its natural form, and its structure has been elucidated by analysis of NMR, MS, and ECD data. Acetylation of the sponge extract facilitated isolation of additional thorectidiol diacetate stereoisomers and the isolation of the racemic monomer thorectidol acetate ().

Three new indole diterpenoids from KKU-CT2.

Three new indole diterpenoids, aculeatupenes A-C (-), together with four known compounds (-), were isolated from the mycelium of KKU-CT2. Their structures were established by spectroscopic evidence and absolute configurations of - were determined by comparison of their experimental and calculated ECD spectra. Compounds , , and emindole SB () showed weak cytotoxicity against HelaS3, KB, HepG2, MCF-7, and A549 cancer cell lines with IC values in the range of 11.

How Long Can A C-C σ-Single Bond Be?

In a specifically designed molecular structure, two sp-hybridized carbon atoms align their unhybridized 2p orbitals in the same orientation to form an extremely long C-C σ-single bond that goes beyond 3 Å in length. This new type of C-C σ-bond (coined as a fringe bond) can be made more than 1 Å longer than the current experimental world record (∼1.8 Å) and 300 kJ/mol weaker than the ordinary C-C σ-bond (∼330 kJ/mol).

[Genetic diversity and volatile components of Asarum sieboldii in Qin-ba region].

Objective: To analyze the genetic diversity and the volatile components of Asarum sieboldii from seven habitats in Qin-ba region.

Methods: The genetic diversity of the herb was analyzed by ISSR (inter simple sequence repeat) markers; The relative content volatile components of the herb were dectected by head space solid-phase microextraction gas chromatogrphy-mass spectrometry (HS-SPME-GC-MS). The contents of the 3 main components were analyzed by steam distillation gas chromatography-mass spectrometry (GC-MS).

[The volatile components of three radix et rhizoma asari].

Objective: To analyze and compare the volatile components of Asarum sieboldii Miq, Asarum himalaicum Hook. F. et Thoms and Asarum.

Cardiac muscle sarcolemma chromatographic stationary phase and its potential application in drug screening.

A new bioactive packing material for liquid chromatography, sarcolemma chromatography stationary phase (SCSP), is presented. Its surface characteristics are investigated, and it is found that the acceptors embedded in sarcolemma remained bioactive for more than a week. The retention behavior of antagonists and activators related to cardiac muscle sarcolemma on the SCSP chromatographic column shows the screening function of the SCSP column, and the retention behavior of the active components in the aether extract from the Chinese herb Ligusticum chuanxiong Hort.

[Study on the interactions between Ligusticum chuanxiong extract and cardiac muscle membrane receptors by CMSP chromatography].

Objective: To study the interactions between Ligusticum chuanxiong Hort extract and cardiac muscle membrane receptors.

Method: The cell membrane of rabbit cardiac muscle was fixed on silicon to make cell membrane stationary phase (CMSP), and then the interactions were studied by comparing the retention characteristics of the extracts from different solvents with those of the antagonists or activators corresponding to known receptors in cardiac muscle membrane, and by competition effect on the retention characteristics of extracts when adding the antagonists or activators into the mobile phase.

Result: Water extract and ethanol extract both had retentions on CMSP; the retention characteristics of water extract could be affected when water extract was in competition with the antagonists for alpha receptor, and could not be affected when with the activator beta1 receptor.