Tunable random polymer fiber laser.

We have demonstrated the realization of on-line temperature-controlled random lasers (RLs) in the polyhedral oligomeric silsesquioxanes (POSS) nanoparticles (NPs) as well as Pyrromethene 597 (PM597) laser dye, FeO/SiO NPs as well as PM597, and only PM597 doped polymer optical fibers (POFs), respectively. The RLs can be obtained from the gained POFs system caused by multiple scattering of emitted light. The refractive index of the fiber core materials can be easily tuned via temperature due to the polymer with large thermo-optic coefficient.

[Expression and purification of Toxoplasma gondii GRA4 gene in prokaryotic system].

Objective: To construct a prokaryotic expression system containing the dense granule protein 4 (GRA4) of Toxoplasma gondii, purify the expressed protein and detect its immunogenicity.

Methods: The specific fragment of GRA4 gene was amplified by PCR. After subcloning the prokaryotic expression recombinant pET-GRA4, the expressed product was purified with His Bind affinity chromatography and analyzed by Western blot.

[High efficiency expression and antigenicity analysis of the SAG2 gene from Toxoplasma gondii RH strain].

Objective: To make high efficiency expression of the SAG2 gene from Toxoplasma gondii RH strain in E. coli and study the antigenicity of the expressed product.

Methods: The SAG2 gene fragment of T.