[An analysis on genetic characterization of HA1 gene of influenza virus subtype H3N2 circulated from 2001 to 2006 in Liaoning local area].

Objective: To understand the HA1 genetic variation characterization of influenza virus subtype H3N2 circulated from 2001 to 2006 in Liaoning local area.

Methods: Viral RNA was extracted and transcribed into cDNA by reverse transcriptase and amplified by PCR. The product of PCR was purified by QIAgen purification kits,and sequenced by ABI 3100avant.

[Influenza surveillance from 1999 to 2005 in Liaoning regions].

Objective: To investigate the prevalence and subtypes of influenza viruses in Liaoning regions from November 1999 to March 2005.

Methods: Influenza virus was isolated by embryonated eggs together with cell culture and subtypes, identified by HI test.

Results: During the study in 1999 - 2005, a total number of 2713 swab specimens were collected in different cities in Liaoning regions in which 188 strains were identified for influenza viruses with an average rate as 7.