Reconstruction of large defects of anterior floor of mouth with free flaps using a novel individualized flap design method.

The purpose of this study was to introduce a novel individualized flap design method for large anterior floor of the mouth (AFOM) defect reconstruction, review experience with the use of this flap design method for large AFOM defect reconstruction, and assess its functional results. A retrospective study of patients who received large AFOM defect reconstruction with free flaps was conducted. There was a cohort of patients who were treated using the novel individualized flap design method and a cohort without flap design.

A novel flap design technique for subtotal tongue reconstruction with an "Individualized and Convenient Tongue Model".

Background: To achieve improved functional outcomes in subtotal tongue reconstruction, a flap design with sufficient volume and appropriate shape is necessary. In this study, we introduce an "Individualized and Convenient Tongue Model" (ICTM) for flap design in subtotal tongue reconstruction.

Methods: By studying the anatomical morphology of the tongue, we found a similar geometry within the dorsum and body of the tongue as well as the mouth floor.

[Construction of Vectors Expressing Inhibiting Peptides for Nuclear Import and Its Effect on Growth and Migration of HeLa Cell].

Objective: To construct the expression vectors for red fluorescent protein fused with inhibiting peptides for nuclear import (Bimax),and explore the location of Bimax and its potential effects on cell proliferation and migration in HeLa cells.

Methods: Two kinds of polynucleotide encoding inhibiting peptides for nuclear import were synthesis respectively and subsequently annealed for inserting into vector pDs-Red-C1. The recombinant plasmids were transfected into competent bacterial DH-5α.

[Emissions of greenhouse gas and ammonia from the full process of sewage sludge composting and land application of compost].

There is a great uncertainty of greenhouse gas (GHG) reduction and nitrogen conservation from the full process of sludge composting and land application of compost in China due to the lack of emission data of GHG such as N2O and CH4 and ammonia (NH3). The purpose of this study is to get emission characteristics of GHGs and NH3 from the full process with on-site observation. Results showed that the total GHG emission factor from full process of the turning windrow (TW) system (eCO2/dry sludge, 196.

[Construction and identification of eukaryotic expression plasmid expressing siRNA targeting USP22 gene].

Objective: To construct eukaryotic expression plasmid expressing siRNA targeting ubiquitin specific peptidase 22 gene (USP22), and to investigate its effect on the growth of hepatoma carcinoma cells HepG2.

Methods: siRNA templates were synthesized based on USP22 mRNA sequence and cloned into vector Pmscv/Hyg/U6. The resulting recombinant was identified by restriction enzyme digestion and DNA sequencing.

[Kinetic characteristics of Cr(III) oxidation by delta-MnO2].

The kinetics of Cr(III) oxidation by synthesized vernadite(delta-MnO2) was investigated through magnetic stirring and fractional centrifugation. The oxidation procedure of Cr(III) by delta-MnO2 could be divided into two first-order reactions, a fast reaction followed by a slow one. In high Cr(III) concentration solution, the reaction was also well sub-simulated with diffusion equation and Elovich equation according to the reaction phases.

Silencing of Ref-1 expression by retrovirus-mediated shRNA sensitizes HEK293 cells to hydrogen peroxide-induced apoptosis.

Redox factor-1 (Ref-1) is a multifunctional protein involved in DNA base excision repair (BER) and transcription factor modification. By the use of retrovirus-delivered shRNA, we effectively suppressed endogenous Ref-1 expression in human embryonic kidney (HEK) 293 cells. Our results showed that downregulation of Ref-1 rendered cells more sensitive to H(2)O(2)-induced apoptosis.

A soluble truncated cadherin induces breast cancer cell apoptosis and growth inhibition.

Purpose: To identify the characteristics and function of the truncated cadherin cDNA which encodes a soluble molecule containing the sequence of VE-cadherin extracellular domain repeats from repeat 1 to 4 (designated as CED1-4) and a secreting signal peptide at N terminal.

Methods: A pMSCV/CED1-4 vector was constructed. Recombinant retrovirus ReCED1-4 and ReEmpty were produced by 293 package cells and transfected into MDA-MB435 human breast cancer cells.