[Preparation and evaluation of a time-resolved fluoroimmunoassay kit for CA50 determination].

Objective: To prepare a rapid and sensitive diagnostic kit for detection of CA50 based on time-resolved fluoroimmunoassay.

Methods: A sandwich-TRFIA diagmostic kit was developed using anti-CA50 monoclonal antibody and all parameters of the kit were evaluated.

Results: The linear measurement range of the kit was (5 - 300) U/ml.

[Time-resolved fluoroimmunoassay of carcino-embryonic antigen and preparation of its diagnostic reagent].

Aim: To establish a two-site time-resolved fluoroimmunoassay (TRFIA) for detection of carcino-embryonic antigen(CEA) based on the direct sandwich technique(One monoclonal antibody(mAb) is immobilized on the surface of microtiter plate strip wells, another mAb is labelled with Eu(3+)) and prepare its diagnostic reagent.

Methods: The sandwich TRFIA for detection of serum CEA level using anti-CEA mAbs was used.

Results: The measure range of CEA-TRFIA was (1-560) microg/L.

[Preparation of time-resolved fluoroimmunoassay kit for human alpha-fetoprotein detection].

Objective: To prepare time-resolved fluoroimmunoassay (TRFIA) kit for detecting human alpha-fetoprotein (hAFP).

Methods: Sandwich TRFIA kit for hAFP detection was developed using monoclonal antibody of anti-AFP.

Results: AFP-TRFIA kit was capable of detecting AFP within the range of 1-1 000 U/ml with sensitivity of 0.