Quantitative secretome analysis of polymyxin B resistance in Escherichia coli.

Bacterial resistance has become a serious threat to human health. In particular, the gradual development of resistance to polymyxins, the last line of defense for human infections, is a major issue. Secreted proteins contribute to the interactions between bacteria and the environment.

[Mass spectrometry identification and immune cross-reactivity of a minor shrimp allergen-sarcoplasmic calcium binding protein from Litopenaeus vannamei].

Aim: To identify sarcoplasmic calcium-binding protein (SCP) as a minor shrimp allergen by mass spectrometry, and to analyze the immune cross-reactivity among crustacean SCPs.

Methods: The M(r); 21 000 allergen from Litopenaeus vannamei was identified by MALDI-TOF/TOF-MS. BLAST and ClustalW were used to compare amino acid sequence identity of the allergen among crustaceans.

[Identification, isolation, purification of allergens in shrimp and allergic analysis for the main allergens].

Aim: To identify the allergens in shrimp, and to isolate, purify and analyze the main allergen components.

Methods: The total shrimp proteins were extracted by PBS, the allergens were identified with 11 shrimp allergic patients' serum IgE by Western blot. Three main shrimp allergens 21,000, 36,000 and 80,000 were purified by ammonium sulfate precipitation, Sephadex G-50 chromatography and DEAE-exchange chromatography.