[Preparation and identification of polyclonal antibody against cysteinyl leukotriene receptor 2].

Objective: To prepare and identify a polyclonal antibody against cysteinyl leukotriene receptor (CysLT(2)receptor).

Methods: Rabbits were immunized with KLH-coupled CysLT(2) receptor peptide to prepare the polyclonal antibody (pAb). The titer of the pAb in rabbit plasma was detected by indirect ELISA, and the specificity of the pAb was tested by antigen blockade.

[Preparation and identification of a polyclonal antibody against novel cysteinyl leukotriene receptor GPR17].

Objective: To prepare and identify a polyclonal antibody (pAb) against GPR17, a novel cysteinyl leukotriene receptor.

Methods: Rabbits were immunized with KLH-coupled GPR17 peptide to prepare the pAb. The titer of the pAb in rabbit plasma was detected by indirect ELISA, and the specificity of the pAb was tested by antigen blockade.

[Construction of bi-cistronic co-expression plasmid of mIL-12 and the expression in vitro or in vivo].

OBJECTIVE: To construct a bi-cistronic co-expression plasmid for mouse interleukin-12 and to observe its expression in vitro or in vivo.METHODS: The full-length cDNA encoding p35 and p40 was cloned into eukaryotic cells expression vector pcDNA 3.1 respectively.