Immune Cell Landscape in Gastric Cancer.

Background: The tumor-infiltrating immune cells are closely associated with the prognosis of gastric cancer (GC). This article is aimed at determining the composition change of immune cells and immune regulatory factors in GC and normal tissues, depicting their prognosis value in GC, and revealing the relationship between them and GC clinical parameters.

Methods: We used CIBERSORT to calculate the proportion of 22 immune cells in the GC or normal tissues; a -test was applied to assess the expression difference of immune cells and immune regulatory factors in normal and GC tissues.

[Effects and action mechanisms of apocynin in the treatment of oligoasthenozoospermia in the mouse].

Objective: To investigate the therapeutic effect of apocynin on oligoasthenozoospermia (OAS) and its action mechanisms in mice.

Methods: Forty male Kunming mice were randomly divided into four groups of equal number, blank control, OAS model, low-dose apocynin, and high-dose apocynin. OAS models were made in the latter three groups of mice by intraperitoneal injection of cyclophosphamide for five days.

Subcutaneous Injections of the Mannose-Sensitive Hemagglutination Pilus Strain of Pseudomonas aeruginosa Stimulate Host Immunity, Reduce Bladder Cancer Size and Improve Tumor Survival in Mice.

We wished to evaluate the effects of Pseudomonas aeruginosa (mannose-sensitive hemagglutination pilus strain, PA-MSHA) as an immunostimulating and anti-tumor agent for treatment of bladder cancer. Immunostimulating effects were assessed by the in vitro proliferation assay of murine splenic lymphocytes. Anti-tumor effects were studied in a subcutaneous tumor model established in female C57BL/6 mice using the MB49 bladder cell line.

[Endemic situation of schistosomiasis after transmission-interrupted in debao county: a 25-year surveillance].

Objective: To understand the dynamics of schistosomiasis prevalent factors after the transmission interruption in Debao Country, so as to provide the evidence for establishing a control strategy.

Methods: By using the system sample method combined with environmental sample method, the Oncomelania hupensis snail situation was surveyed through the general survey, round survey and selection check. Schistosomiasis cases were detected from local population and floating population by serological tests and stool tests.

Bisphosphonates for osteoporosis in nonmetastatic prostate cancer patients receiving androgen-deprivation therapy: a systematic review and meta-analysis.

This systematic review was conducted to assess the efficacy and safety of bisphosphonates for prevention and treatment of osteopenia or osteoporosis in men with non-metastatic prostate cancer receiving androgen- deprivation therapy. We searched for randomised controlled trials (RCTs) of bisphosphonates compared with placebo from Pubmed, Embase, the Cochrane Library, and ISI - Science Citation Index. Meta-analyses of pre- specified outcomes (bone mineral density, fractures, and adverse events) were performed using Review Manager.

Highly sensitive chemiluminescence technology for protein detection using aptamer-based rolling circle amplification platform.

A robust, selective and highly sensitive chemiluminescent (CL) platform for protein assay was presented in this paper. This novel CL approach utilized rolling circle amplification (RCA) as a signal enhancement technique and the 96-well plate as the immobilization and separation carrier. Typically, the antibody immobilized on the surface of 96-well plate was sandwiched with the protein target and the aptamer-primer sequence.

Zeolite-based cataluminescence sensor for the selective detection of acetaldehyde.

The reactions of acetaldehyde with O atoms in the cages of large-pore zeolites have been discovered to result in light emission. The luminescence characteristics of acetaldehyde vapours passing through the surface of chosen zeolites were studied using a cataluminescence-based detection system. To demonstrate the feasibility of the method, the detection of acetaldehyde using catalysts was studied systematically and a linear response of 0.

In vitro enhanced cytotoxicity of tumor-infiltrating lymphocytes transfected with tumor necrosis factor-related apoptosis-inducing ligand and/or interleukin-2 gene in human renal cell carcinoma.

Objectives: To investigate whether tumor-infiltrating lymphocytes (TILs) transfected with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and interleukin-2 (IL-2) genes are capable of improving the potency and efficacy of propagation and cytotoxicity against renal cell carcinoma (RCC) cells in vitro.

Methods: A mammal expression vector system was constructed. TILs were transfected by liposome-mediated gene transfection.

[Simultaneous determination of isomers of dihydroxybenzene using alternating trilinear decomposition algorithm combined with reversed-phase high performance liquid chromatography/diode array detection].

Alternating trilinear decomposition (ATLD) algorithm is a promising method for second-order calibration, which is based on an alternating least-squares principle to overcome some shortcomings of the traditional parallel factor analysis (PARAFAC) algorithms, such as the sensitivity to the estimated component numbers and slow convergence. It utilizes an iterative procedure involving Moore-Penrose generalized inverse computations based on singular value decomposition and can be applied to the simultaneous determination of several organic components even in the presence of unknown interfering components. The overlapped chromatogram and spectra system of dihydroxybenzenes were resolved by ATLD combined with reversed-phase high performance liquid chromatography (RP-HPLC)/ diode array detection (DAD).

Urothelial injury to the rabbit bladder caused by calcium dissolving agents including two new citrate solutions.

We compared the urothelial injury to the bladder caused by four agents capable of dissolving calcium salts. The solutions were administrated in an antegrade way through left ureterostomies in 54 rabbits for periods of 24, 48 and 72 h. The bladders were then removed and three routine histological sections were made for each.

New chemolysis for urological calcium phosphate calculi - a study in vitro.

Background: Advances in techniques have left very few indications for open surgical extraction of urinary stones currently. These advances notwithstanding, the search continues for medical approaches to urinary stone management. In this study, we perform an in vitro study analyzing the efficiency and prospect of two new complex solutions in urological calcium phosphate calculi dissolution.

[Clinical observation of the lead discharging effect of paiqian chewing tablet].

Objective: To observe the effect of paiqian chewing tablet (PQCT) on lead discharging and health in children.

Methods: Adopting self-control and inter-group control method, 94 children with blood lead level exceeding 100 microg/L were randomly divided into the observed group and the control group. The observation period for both groups was 30 days.

An improved trilinear decomposition algorithm based on a Lagrange operator.

An improved trilinear decomposition algorithm based on a Lagrange operator (LO) is developed in this paper, which introduces a Lagrange operator and penalty terms in the loss function to improve the performance of the algorithm. Compared to the traditional parallel factor (PARAFAC) algorithm, the algorithm not only may converge much faster, but also overcome the sensibility to estimate the number of components. A set of simulated and measured excitation/emission fluorescence data were treated by both the proposed and traditional PARAFAC algorithm to compare their efficiencies.

Effects of reactive oxygen species on lymphokine-activated killer cells in patients with bladder cancer.

Aim: To investigate the effect of reactive oxygen species on the proliferation of lymphokine-activated killer (LAK) cells in patients with bladder cancer and their cytolysis to bladder tumor cells.

Methods: Sodium nitroprusside (SNP) was used as nitric oxide (NO) donor. The superoxide anion (O2-.