Effects of on the Structure and Physicochemical Properties of Starch in Developing Rice Seeds.

Overexpression of a constitutively active truncated form of () in rice produced smaller seeds, but a double-stranded RNA gene-silenced form of () yielded larger seeds, suggesting that plays a functional role in rice seed development. In the study presented here, we propose a model in which plays key roles in negatively controlling the grain size, amylose content, and endosperm appearance, and also affects the physicochemical properties of the starch. The dehulled transgenic grains were smaller than the dehulled wild-type grains, and the endosperm was opaque and had a low amylose content and numerous small loosely packed polyhedral starch granules.

Overexpression of a constitutively active truncated form of OsCDPK1 confers disease resistance by affecting OsPR10a expression in rice.

The rice pathogenesis-related protein OsPR10a was scarcely expressed in OsCDPK1-silenced (Ri-1) rice, which was highly sensitive to pathogen infection. After inoculating the leaves with bacterial blight (Xanthomonas oryzae pv. oryzae; Xoo), we found that the expression of OsPR10a was up- and down-regulated in OEtr-1 (overexpression of the constitutively active truncated form of OsCDPK1) and Ri-1 rice plants, respectively.

Multiple Patterns of Regulation and Overexpression of a Ribonuclease-Like Pathogenesis-Related Protein Gene, OsPR10a, Conferring Disease Resistance in Rice and Arabidopsis.

An abundant 17 kDa RNase, encoded by OsPR10a (also known as PBZ1), was purified from Pi-starved rice suspension-cultured cells. Biochemical analysis showed that the range of optimal temperature for its RNase activity was 40-70°C and the optimum pH was 5.0.