[Construction of Vectors Expressing Inhibiting Peptides for Nuclear Import and Its Effect on Growth and Migration of HeLa Cell].

Objective: To construct the expression vectors for red fluorescent protein fused with inhibiting peptides for nuclear import (Bimax),and explore the location of Bimax and its potential effects on cell proliferation and migration in HeLa cells.

Methods: Two kinds of polynucleotide encoding inhibiting peptides for nuclear import were synthesis respectively and subsequently annealed for inserting into vector pDs-Red-C1. The recombinant plasmids were transfected into competent bacterial DH-5α.

[Study on formulation of Scutellaria baicalensis extract phospholipid complex nasal preparation].

Objective: To screen suitable nasal dosage forms of Scutellaria baicalensis extract phospholipid complex.

Methods: Used the permeable performance of hyorrinis mucosa, nasal irritation and other evaluation indexes to screen five different formulations.

Results: The submicroemulsion's apparent permeability coefficient was the largest, irritant was the smallest, and drug loading was higher than those of other formulations, which could meet the requirements of intranasal administration.

[Powder modification technology used for the preparation of the hydrophilic decoction pieces of indigo naturalis and the modification principle].

The hydrophilicity of the normal decoction pieces (NDP) of Indigo Naturalis is not good, therefore, it is not suit for decoctions. In this paper, powder modification technology is used and some NDP and alcohol are ground together in the vibromill to prepare the hydrophilic decoction pieces (HDP) of Indigo Naturalis. Initially, the properties of NDP, ultrafine decoction pieces (UDP) and HDP are compared, the hydrophilicity of UDP was promoted slightly, that of HDP is promoted dramatically.