Modified Sequential Therapy Regimen versus Conventional Triple Therapy for Helicobacter Pylori Eradication in Duodenal Ulcer Patients in China: A Multicenter Clinical Comparative Study.

Objective. Antimicrobial resistance has decreased eradication rates for Helicobacter pylori infection worldwide. To observe the effect of eradicating Helicobacter pylori (H.

The eradicating Helicobacter pylori infection in duodenal ulcer patients by three short-term triple therapies in China: a multicenter clinical comparative study.

Background/aims: This study aimed to compare the 7d triple therapy with 3d and 5d triple therapies, to observe the effect of eradicating Helicobacter pylori (Hp) on treating duodenal ulcers.

Methodology: One hundred and sixteen patients who were confirmed duodenal ulcer active period and Hp positive were enrolled in the study. All the patients were divided into three groups: 3d group (n=39), 5d group (n=37) and 7d control group (n=40).

Cyclopamine blocked the growth of colorectal cancer SW116 cells by modulating some target genes of Gli1 in vitro.

Background/aims: Hedgehog (Hh) pathway has been considered as a therapy target for various cancer entities. However, its mechanism in colorectal cancer is still unclear.

Methodology: We analyzed the expression of Hh pathway members in colorectal adenomas and cancer cell lines and then studied its relationship with survival of colorectal cancer cells through inhibiting Hh pathway by cyclopamine.

[Study on patients with benign prostate hyperplasia treated by the therapeutic regimen of watchful waiting during a twenty-four-month follow-up period].

Objective: To analyze the changes of the main clinic parameters in patients with benign prostate hyperplasia (BPH) treated by watchful waiting and to find out the risk factors contributing the progress of BPH.

Methods: According to the inclusion and exclusion criteria,61 patients diagnosed as BPH were recruited in the group of watchful waiting. Data on IPSS, prostate volume, prostate specific antigen (PSA), maximum flow rate, average flow rate and residual urine volume during follow-up period of 24 months, were recorded.

[Data analysis of the objective observation parameters of benign prostatic hyperplasia patients accepting different treatment regimens].

Objective: To observe different objective observation parameters of the benign prostatic hyperplasia (BPH) patients accepting different treatment strategies, and to further analyze the relationship of these factors with the treatment option.

Methods: Three hundred and twenty-nine BPH patients, aged 50-80, were assigned into 3 groups jointly decided by the physicians and patients based on the individual conditions and the patients' willingness: watchful waiting group (n=61), aged (63 +/- 8), drug treatment group (n=179), aged (68 +/- 7), and operation group (n=89), aged (71 +/- 6). The data of prostate volume, prostate specific antigen (PSA), maximum flow rate (Qmax), average flow rate, urinating volume, and residual urine volume before treatment were recorded.

PCR-SSCP-DNA sequencing method in detecting PTEN gene mutation and its significance in human gastric cancer.

Aim: To discuss the possible effect of PTEN gene mutations on occurrence and development of gastric cancer.

Methods: Fifty-three gastric cancer specimens were selected to probe PTEN gene mutations in genome of gastric cancer and paracancerous tissues using PCR-SSCP-DNA sequencing method based on microdissection and to observe the protein expression by immunohistochemistry technique.

Results: PCR-SSCP-DNA sequencing indicated that 4 kinds of mutation sites were found in 5 of 53 gastric cancer specimens.

Effects of endothelin-1 on hepatic stellate cell proliferation, collagen synthesis and secretion, intracellular free calcium concentration.

Aim: To explore the effects of endothelin-1(ET-1) on hepatic stellate cells (HSCs) DNA uptake, DNA synthesis, collagen synthesis and secretion, inward whole-cell calcium concentration ([Ca2+]i) as well as the blocking effect of verapamil on ET-1-stimulated release of inward calcium (Ca2+) of HSC in vitro.

Methods: Rat hepatic stellate cells (HSCs) were isolated and cultivated. 3H-TdR and 3H-proline incorporation used for testing DNA uptake and synthesis, collagen synthesis and secretion of HSCs cultured in vitro; Fluorescent calcium indicator Fura-2/AM was used to measure [Ca2+]i inward HSCs.

Clinical evaluation of four one-week triple therapy regimens in eradicating Helicobacter pylori infection.

Aim: To evaluate clinical efficacy of four one-week triple therapies in eradicating Helicobacter pylori infection.

Methods: In this clinical trial, 132 patients with duodenal ulcer and chronic gastritis were randomly divided into four groups, and received treatment with OAC (omeprazole 20 mg + amoxicillin 1 000 mg + clarithromycin 250 mg), OFC (omeprazole 20 mg + furazolidone 100 mg + clarithromycin 250 mg), OFA (omeprazole 20 mg + furazolidone 100 mg + amoxicillin 1 000 mg) and OMC (omeprazole 20 mg + metronidazole 200 mg + clarithromycin 250 mg), respectively. Each drug was taken twice daily for one week.