Publications by authors named "Jian-Xun Liu"

To explore the regulation of vasodilatory function in rats with post-infarction heart failure by salvianolic acid B(Sal-B) based on the mechanosensitive ion channel, namely Piezo1. A post-infarction heart failure model of rats was prepared by ligation of the left anterior descending coronary artery. After successful modeling, the rats were randomly divided into the model group, Sal-B group(0.

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  • * MIRI is mainly caused by oxidative stress from reactive oxygen species (ROS), which disrupts cellular balance and causes significant cell damage, inflammation, and dysfunction in heart cells.
  • * Recent studies have made progress in understanding oxidative stress in heart injury, but the protective effects of Traditional Chinese Medicine (TCM) against this damage during blood flow restoration remain largely unexamined.
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  • This study investigated how tetrahydropalmatine (THP) protects heart cells from damage caused by hypoxia/reoxygenation (H/R) injury by inhibiting mitophagy through specific cellular pathways (ULK1/FUNDC1).
  • H9c2 heart cells were used to model H/R injury, and various methods were employed to evaluate cell viability, mitochondrial function, and the expression of key proteins related to autophagy and apoptosis.
  • Results showed that THP improved mitochondrial health by lowering reactive oxygen species levels, enhancing ATP production, and reducing cell death, suggesting that it achieves this by inhibiting autophagy and decreasing apoptosis.
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  • The article examines how ginsenoside Re helps protect H9c2 cells from hypoxia/reoxygenation (H/R) injury by regulating mitochondrial biogenesis through a specific pathway involving Nrf2, HO-1, and PGC-1α.
  • The study found that ginsenoside Re improves cell activity and reduces oxidative stress markers like superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and reactive oxygen species levels after H/R injury.
  • It also demonstrated that ginsenoside Re enhances mitochondrial function by improving mitochondrial membrane potential, decreasing permeability, and increasing key protective protein expressions, ultimately reducing apoptosis-related signals in the cells.
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  • Macrophages are crucial immune cells that help maintain balance in the lung environment and can change into two types: M1 (pro-inflammatory) and M2 (anti-inflammatory), influencing pulmonary diseases.
  • The paper discusses how the polarization of these macrophages, affected by various signaling molecules, relates to conditions like asthma, chronic obstructive pulmonary disease, acute lung injury, and pulmonary fibrosis.
  • It also reviews advancements in using traditional Chinese medicine (TCM) to regulate macrophage polarization, highlighting its potential in managing lung inflammation and inspiring future clinical research.
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  • - SSNX is a traditional Chinese medicine used to treat myocardial ischemia-reperfusion injury (MIRI), which has shown promise in reducing cardiovascular events, though its specific mechanisms in protecting microvascular injury are not fully understood.
  • - The study aims to explore how SSNX protects the microvasculature in rats and whether this protection is linked to the NR4A1/Mff/Drp1 pathway during I/R injury.
  • - Various experimental methods, including histological staining, Western blotting, and cell culture models, were employed to assess cardiac function, evaluate microvascular health, and investigate the underlying molecular mechanisms involving mitochondrial function.
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  • - This study examines how baicalein (BAI) affects inflammatory responses in human microglial cells (HMC3) induced by lipopolysaccharide (LPS) and its potential therapeutic benefits for ischemic stroke through the cAMP-PKA-NF-κB/CREB pathway.
  • - Researchers tested various doses of BAI on LPS-induced inflammation in HMC3 cells, measuring key indicators of oxidative stress and inflammatory markers, and found that BAI improved cell health and reduced inflammation at certain concentrations.
  • - The study highlights the promise of BAI as a treatment derived from traditional Chinese medicine for cerebral ischemic diseases by showing its ability to protect against inflammation and promote cell recovery.
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  • Neural stem cells (NSCs) can generate new neurons to repair brain damage after injury, but their ability to do so is often limited due to high cell death rates before maturation.
  • Small molecules, like Senkyunolide I (SEI), derived from traditional Chinese medicine, show promise in enhancing the proliferation of NSCs and treating conditions like ischemic stroke.
  • The study found that while SEI increases the number of proliferating NSCs, it also inhibits their differentiation into mature neurons or astrocytes, suggesting a potential for SEI in boosting endogenous NSCs for brain repair.
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  • This study evaluated the impacts of Buyang Huanwu Decoction (BYHWD) on platelet activation and gene expression in rats following acute myocardial infarction (AMI).
  • The research found that BYHWD treatment led to improved heart functions, reduced platelet activation, and better blood circulation as compared to the model group, which had experienced AMI without treatment.
  • Additionally, gene expression analysis indicated that BYHWD modified important pathways related to platelet function, suggesting potential protective effects against heart damage during AMI.
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  • The study aimed to assess the effectiveness of Huoxin Pill (HXP) in protecting against heart damage during myocardial ischemia-reperfusion injury in rats.
  • Rats were divided into various groups for treatment, and after a week of administration, they underwent a procedure to simulate heart injury, followed by measurements of specific biomarkers and heart damage assessment.
  • Results showed that HXP at all dosages significantly decreased harmful biomarker levels and improved heart condition compared to the untreated model group.
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To optimize the extraction process of Chuanxiong Rhizoma-Gastrodiae Rhizoma herb pair by network pharmacology combined with analytic hierarchy process(AHP)-entropy weight method and multi-index orthogonal test. The potential active components and targets of Chuanxiong Rhizoma-Gastrodiae Rhizoma were screened by network pharmacology and molecular docking, and the process evaluation indexes were determined with reference to the Chinese Pharmacopoeia(2020 edition). The core components of Chuanxiong Rhizoma-Gastrodiae Rhizoma were determined as gastrodin, parishin B, parishin C, parishin E, ferulic acid, and 3-butylphthalide.

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Large-artery atherosclerosis type of ischemic stroke happens when a blood clot forms in a major artery that carries blood to the brain. This causes a blockage and a decrease in blood flow to the brain tissue making up approximately 15-20% of all cases. This type of stroke is more prevalent in older adults and those with risk factors such as high blood pressure, high cholesterol, diabetes, smoking, and a family history of stroke.

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Vascular dementia (VaD) accounts for 15-20% of all dementia cases. It is a syndrome of acquired cognitive impairment with a complex pathophysiological basis. A novel herbal formulation (Sailuotong; SLT) consisting of Panax ginseng C.

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Piezo1 channel is a mechanosensitive ion channel that opens in response to shear stress, tension, torsion and a series of mechanical stimulation and turns them into biological signals. It plays an important role in vascular development, vasoconstriction and vasodilation, formation of arterial and venous valves, blood pressure regulation and red blood cell homeostasis, and thus is closely related to cardiovascular system. Recent studies have shown that traditional Chinese medicine(TCM) compounds and their active components can affect Ca~(2+) influx and endothelial cell and platelet function by mediating Piezo1 channel, and regulate thrombosis and endothelial homeostasis, thereby treating cardiovascular diseases.

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This study aims to observe the therapeutic effect of salidroside on cerebral ischemia-reperfusion(I/R) model rats, and to specifically explore the protection of salidroside on endothelial cell barrier after I/R and the mechanism. In the experiment, SD rats were randomized into sham group, model group, and high-, medium-, and low-dose(10, 5, and 2.5 mg·kg~(-1)) salidroside groups.

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Cardiovascular diseases are a global public health problem, and the combination of Chinese and western medicine tends to be a major solution in China. However, the complex components in traditional Chinese medicine may interact with the therapeutic western medicines for the diseases, which will lead to the herb-drug interaction(HDI). The information on the interaction can serve as a reference for the rational combination of the Chinese and western medicines in the clinical treatment of cardiovascular diseases and help avoid the occurrence of clinical safety events.

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Panax notoginseng saponins (PNS), the main active ingredient of herbal medicine Panax notoginseng, has been generally applied for the therapy of cardiovascular and cerebrovascular diseases, especially for stroke. It is believed that PNS has obvious anti-inflammatory effect, however, the roles of PNS on microglia after stroke have not been completely explored and the underlying mechanism of microglia-mediated inflammation remains to be clarified. In this study, cerebral ischemia injury was induced by photothrombotic (PT) stroke in mice.

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Two previously undescribed steroidal alkaloids, compounds 1-2, along with two known ones(3-4), were isolated from the 80% ethanol extract of ripe berries of Solanum nigrum by chromatographic methods, including silica gel, ODS, and HPLC. Based on spectroscopic and chemical evidence, including IR, NMR, and HR-ESI-MS data, the structures of the isolated compounds were identified as 12β,27-dihydroxy solasodine-3-O-β-D-glucopyranoside(1), 27-hydroxy solasodine-3-O-β-D-glucopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside(2), solalyraine A(3), and 12β,27-dihydroxy solasodine(4). Compounds 1-2 were tested for their potential effects against the proliferation of A549 cells, which revealed that compounds 1-2 had weak cytotoxic activity.

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This study aims to explore the effect of Jinzhen Oral Liquid(JOL) on cough after infection in rats and the mechanism. To be specific, a total of 60 male SD rats were classified into 6 groups: normal group(equivalent volume of distilled water, ig), model group(equivalent volume of distilled water, ig), Dextromethorphan Hydrobromide Oral Solution group(3.67 mL·kg~(-1), ig), high-, medium-, and low-dose JOL groups(11.

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This study observed the pharmacological effects of Feilike Mixture(FLKM) in stopping cough, eliminating phlegm, and relieving asthma through animal experiments, and explored its mechanism using network pharmacology. The antitussive effect was detected by citric acid-induced guinea pig cough model, the expectorant effect by mouse phenol red excretion experiment and lipopolysaccharide-induced mucus hypersecretion rat model, and the antiasthmatic effect by histamine phosphate-induced guinea pig asthma model. The chemical components of FLKM were collected by TCMSP, TCMID, TCMIP, and BATMAN-TCM databases and literature search, and the potential active components were screened through ADMETlab 2.

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The objective of this study was to evaluate the fertilization capability of White Bengal Tiger frozen-thawed completely immotile spermatozoa after interspecific intracytoplasmic sperm injection (ICSI) with bovine oocytes. The fertilization status of presumptive zygotes was assessed 18 h after ICSI by immunofluorescence staining and confocal microscopy. The fertilization rate was 34.

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This study determined the extraction rates of indirubin in Indigo Naturalis by ethanol reflux extraction method and water extraction method. The pharmacodynamic study against cough induced by ammonia water in the mouse model and the cough induced by citric acid in the guinea pig model were performed to optimize the extraction process of the sovereign medicinal Indigo Naturalis and the whole prescription of Children's Qingfei Zhisou Syrup. The extraction rate of indirubin by the ethanol reflux method was 51.

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Protective effect of Qilong Capsules(QL) on the myocardial fibrosis and blood circulation of rats with coronary heart disease of Qi deficiency and blood stasis type was investigated. Sleep deprivation and coronary artery ligation were used to construct a disease-symptom combination model, and 60 SD rats were divided into sham operation(sham) group, syndrome(S) group, disease and syndrome(M) group and QL group randomly. The treatment group received administration of QL 0.

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This study aims to explore the pharmacodynamic effect of baicalin on rat brain edema induced by cerebral ischemia reperfusion injury and discuss the mechanism from the perspective of inhibiting astrocyte swelling, which is expected to serve as a refe-rence for the treatment of cerebral ischemia with Chinese medicine. To be specific, middle cerebral artery occlusion(suture method) was used to induce cerebral ischemia in rats. Rats were randomized into normal group, model group, high-dose baicalin(20 mg·kg~(-1)) group, and low-dose baicalin(10 mg·kg~(-1)) group.

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Ischemic cardiovascular and cerebrovascular diseases threatening human health and survival have high morbidity and mortality. The common cause of them is reduced blood supply caused by vascular stenosis, atherosclerosis, and infarction. However,the pathological processes of ischemic cardiovascular and cerebrovascular diseases are complex, involving oxidative stress, calcium overload, inflammation, apoptosis, autophagy and other mechanisms.

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