Publications by authors named "Jian-Xiu Ma"
Role of DNA damage response in cyclophosphamide-induced premature ovarian failure in mice.
J Obstet Gynaecol Res
September 2024
Article Synopsis
- The study aimed to explore the DNA damage response (DDR) in mice undergoing premature ovarian failure (POF) induced by cyclophosphamide (CTX).
- The results showed that CTX treatment led to decreased body and ovarian weights, irregular estrus cycles, and hormonal changes, indicating significant ovarian dysfunction in the POF model.
- The findings suggest that CTX-induced POF in mice is linked to the activation of the DDR pathway involving ATM, P53, and P21, highlighting the potential mechanisms behind ovarian failure.
Background: H2AX is phosphorylated (γH2AX) by members of the phosphatidylinositol 3-kinase (PI3K) family, including Ataxia telangiectasia-mutated (ATM), ATM- and Rad3-related (ATR) and DNA-PK in response to DNA damage. Our study shows that gossypol acetic acid (GAA) alone can induce γH2AX in Human mucoepidermoid carcinoma cell line (MEC-1) in vitro. Thus, we further examined the possible mechanisms of GAA to induce γH2AX in tumor cells.
View Article and Find Full Text PDFThe present study was conducted to investigate the effects of gossypol acetic acid (GAA) on the proliferation of human mucoepidermoid carcinoma cell line MEC-1 in vitro and its possible molecular mechanisms of DNA double-strand breaks (DSB). MTT assay was performed to test the inhibition of proliferation of MEC-1 cells by GAA. DSB and γH2AX foci formation induced by GAA were detected by neutral comet assay and immunostaining.
View Article and Find Full Text PDF[Effects of matrine on airway inflammation and early airway remodeling in asthmatic mice].
Zhonghua Jie He He Hu Xi Za Zhi
March 2009
Objective: To observe the influence of matrine on airway inflammation and early airway remodeling in asthmatic mice.
Methods: Fifty BALB/c mice were randomly divided into 5 groups: a normal control group (A), an asthmatic group (B), a dexamethasone (DXM) group (C, 2 mg/kg), a high-dose matrine group (D, 50 mg/kg) and a low-dose matrine group (E, 25 mg/kg). The mice model of asthma in the B, C, D, and E groups was established by ovalbumin (OVA) intraperitoneal injections and aerosolization.