APE1 promotes antioxidant capacity by regulating Nrf-2 function through a redox-dependent mechanism.

APE1 is a multifunctional protein that has recently been implicated in protecting cells from oxidative stress. In the current study, we confirmed that APE1׳s effect on cellular antioxidant capacity is related to its redox activity through the use of an APE1 functional mutant, and we investigated the mechanism through which this multifunctional protein affects the function of the transcription factor Nrf-2 in regulating oxidative stress-induced genes. Using a pair of mutants for both the redox activity and the acetylation-regulated activity of APE1, in vitro assays showed that the redox activity of APE1 is crucial for its nuclear association with Nrf-2 and subsequent activation of Nrf-2׳s transcription of several downstream genes during oxidative challenge.

Genistein alleviates radiation-induced pneumonitis by depressing Ape1/Ref-1 expression to down-regulate inflammatory cytokines.

The aim of the study was to investigate the role of genistein in alleviating radiation-induced pneumonitis(RIP) through down-regulating levels of the inflammatory cytokines by inhibiting the expression of apurinic/apyrimidinic endonuclease 1/redox factor-1 (Ape1/Ref-1). Fifty female C57BL/6J mice (8 weeks old) were randomly divided into a control group, a pure irradiation (IR) group and a genistein + IR group. At the four time points after IR, hematoxylin, and Masson’s trichrome stainings were used to examine the pathological changes and collagen fiber deposition.

[Norman Bethune and field surgery of the Eighth Route Army].

With long-term war experience abroad, combined with the actual situation of health work in China, Bethune put forward a series of strategy and theory used in battlefield conditions of rescuing the wounded in China, such as "fire rescue, early debridement", "emergency blood transfusion in battlefield" and "the crowd blood bank", which effectively improved the rate of saving the battlefield wounded rate in the actual war. Combining with his own practice, he invented a variety of surgical instruments and equipment, such as "lugou bridge" medicine cabinet, "Bipp ointment", which have been widely used in the battlefield. He paid more attention to the construction of battlefield hospital, proposed the establishment of "Model Hospital" and "Special Surgery Hospital" in the rear of Anti-Japanese War, founded the health school, and wrote many battlefield medical books and skills data.

[Effect of substance P on the migration and differentiation of cultured epidermal stem cells to hair follicle in vitro].

Objective: To investigate the effect of substance P (SP) on the migration and differentiation of epidermal stem cells (ESCs) to hair follicle, and its mechanism.

Methods: ESCs were cultured in vitro, and confirmed by positive staining of K19 and integrin beta1 with immunohistochemistry. SP was added into the culture of ESCs which were labelled with 5-BrdU, and the cell cultures were divided into control, 10(-5) mol/L SP, 10(-6) mol/L SP, and 10(-7) mol/L SP groups according to the different doses of SP addition.