[A new method for purification and identification of hepatocellular carcinoma stem cell of SMMC-7721].

Objective: To explore a new efficient purification method of hepatocellular carcinoma (HCC) stem cells and identify their features.

Methods: Human hepatocellular carcinoma cell line SMMC-7721 was cultured in sphere-culture system of polyhedra-treated dish and tumor stem cell specific medium. Upon the formation of cellular sphere, the cells were inoculated subcutaneously into immunocompromised mice and received the interventions of different concentrations of cisplatin.