[Disruption of microfilament cytoskeleton induced by simulated microgravity increases the activity of COL1A1 promoter].

It is well known that cytoskeleton system is the sensor of gravity in cells. Under microgravity condition, cytoskeleton is associated with the changes of cell shape, function, signaling and so on; but the relationship between cytoskeleton and gene expression is not fully understood. In present study, we discussed the effects of cell microfilament on the activity of collagen type I alpha 1 chain gene (COL1A1) promoter under microgravity simulated by clinostat and/or cytochalasin B as microfilament depolymerizer in the established EGFP-ROS cell line using the method of fluorescence semi-quantitative analysis and the fluorescent stain of microfilament.

[Research progress [correction of progresses] of cell culture apparatus].

Cell culture apparatus (CCA) is necessary to space medicine research and space life-science research. With development of technology, CCA has been fully developed. Nowadays, CCA has been used in scientific research and production widely.