[Establishment of a SCID mouse model for synergistic anti-tumor effect of human IL-12 and B7-1].

Human IL-12(hIL-12) has weak effect on mouse immunity cells, so the practical animal model is not available for the study of hIL-12 anti-tumor activity. In this work, the improved Winn assay was applied to evaluate the synergistic anti-tumor effects of hIL-12 and human costimulatory molecule B7-1(hB7-1) on human tumor in HuPBL-SCID mouse model. Three gene transferring solutions hIL-12, hB7-1 and their mixture(1:1) were prepared using the nonliposome transgene reagent and the expressing vectors, and hIL-12, hB7-1 or their mixture were transferred into tumor cell A375 respectively.