Method development and validation of LC-MS/MS-based assay for the simultaneous quantitation of trastuzumab and pertuzumab in cynomolgus monkey serum and its application in pharmacokinetic study.

We present a simple and robust LC-MS/MS assay for the simultaneous quantitation of an antibody cocktail of trastuzumab and pertuzumab in monkey serum. The LC-MS/MS method saved costs, decreased the analysis time, and reduced quantitative times relative to the traditional ligand-binding assays. The serum samples were digested with trypsin at 50°C for 60 min after methanol precipitation, ammonium bicarbonate denaturation, dithiothreitol reduction, and iodoacetamide alkylation.

Surface modification and bioconjugation of FeCo magnetic nanoparticles with proteins.

Magnetic Fe70Co30 nanoparticles with a cubic shape and a mean size of 15±1.5 nm were fabricated using a magnetron-sputtering-based gas phase condensation deposition method. The particles had a high saturation magnetization of 220 emu/g, which is much higher than that of commercially available iron oxide nanoparticles.