Identification of Andrographis Herba and its common products using mini-barcode.

Andrographis Herba, the aerial part of Andrographis paniculata (Burm. f.) Wall.

[Research progress on mechanism of phytohormones in regulating flavonoid metabolism].

Phytohormones play an important role at all stages of plant growth, influencing plant growth and development and regulating plant secondary metabolism, such as the synthesis of flavone, flavonol, anthocyanin, and other flavonoids. Flavonoids, a group of important secondary metabolites ubiquitous in plants, have antioxidative, anti-microbial, and anti-inflammatory activities and thus have a wide range of potential applications in Chinese medicine and food nutrition. With the development of biotechnology, phytohormones' regulation on flavonoids has become a research focus in recent years.

[Regulation of β-mercuryl alcohol metabolic flow in Saccharomyces cerevisiae cells].

In this study, citrate synthase gene(CIT2), and malate synthase gene(MLS1) were successfully knocked out in β-amyrin-producing yeast cells by using CRISPR/CAS9. The promoter of phosphoglucose isomerase gene(PGI1) was replaced by that of cytochrome c oxidase subunit Ⅶa(Cox9)to weaken its expression, aiming to channel more carbon flux into the NADPH-producing pathway. The fermentation results showed that CIT2 deletion had no effect on the β-amyrin production.

[Analysis of shading on DNA methylation by MSAP in Pinellia ternata].

Pinellia ternata is a medicinal herb of Araceae, and its tubers are used as medicines. It is a common Chinese herbal medicine in China and has a large market demand. When exposing to strong light intensity and high temperature during the growth process, P.

[Variation analysis of genomic methylation induced by high temperature stress in Pinellia ternata].

Pinellia ternata belongs to the Araceae family and is a medicinal herb. The tuber is the medicinal organ with antitussive, antiemetic and anti-tumor activities. It is easy to encounter high temperature environment during the growth periods, leading to decrease of tuber production.

[Study of heterologous efficient synthesis of β-amyrin and high-density fermentation].

In this study, the synthetic pathway of β-amyrin was constructed in the pre-constructed Saccharomyces cerevisiae chassis strain Y0 by introducing β-amyrin synthase from Glycyrrhiza uralensis, resulting strain Y1-C20-6, which successfully produced β-amyrin up to 5.97 mg·L~(-1). Then, the mevalonate pyrophosphate decarboxylase gene(ERG19), mevalonate kinase gene(ERG12), 3-hydroxy-3-methylglutaryl-CoA synthase gene(ERG13), phosphomevalonate kinase gene(ERG8) and IPP isomerase gene(IDI1)were overexpressed to promoted the metabolic fluxto the direction of β-amyrin synthesis for further improving β-amyrin production, resulting the strain Y2-C2-4 which produced β-amyrin of 10.

[Rapid identification of Gekko gecko by loop-mediated isothermal amplification based on 12S rRNA sequence].

Gekko gecko (Tokay Gecko) is a valuable traditional Chinese medicine. In this study, the loop-mediated isothermal amplification (LAMP) technique was introduced for visual rapid identification of G. gecko from adulterants.

[Identification of bear bile powder and its adulterants by using DNA barcoding technology].

To identify the precious bile powder and its adulterants by DNA barcoding, and establish its standard experimental process to ensure the safe and effective utilization. Total twelve sequences from samples of bear bile powder which come from Ursus thibetanus for DNA extraction, PCR(polymerase chain reaction) and sequence, then using CodonCode Aligner V 7.0.

[Influence of light on gene expression of key synthesis enzyme genes FtANR and FtLAR about proanthocyanidin in seeds of homologous plant of food and medicine Fagopyrum tataricum].

Tartary buckwheat Fagopyrum tataricum is an important medicinal and functional herb due to its rich content of flavonoids in the seeds. F.tataricum exhibited good functions for free radicals scavenging， anti-oxidation， anti-aging activities.

[Investigation and optimization on ability of enzymatic hydrolysis of Mori Cortex residue].

Residue of Mori Cortex was studied to optimize its enzymatic hydrolysis process, and explore its potential as a carbon source for biochemistry and biofuel production. The cellulose content of diluted acid pretreated (DAP) and non-pretreated from Mori Cortex were measured in this study, and the results showed that the cellulose content of DAP and non-pretreated from Mori Cortex were 52.5% and 47%, respectively.

[Recent advances of synthetic biology for production of functional ingredients in Chinese materia medica].

The functional ingredients in Chinese materia medica are the main active substance for traditional Chinese medicine and most of them are secondary metabolites derivatives. Until now,the main method to obtain those functional ingredients is through direct extraction from the Chinese materia medica. However, the income is very low because of the high extraction costs and the decreased medicinal plants.

Nanocomposites with gold nanorod/silica core-shell structure as saturable absorber for femtosecond pulse generation in a fiber laser.

Ultrafast fiber lasers play a significant role in our society with many aspects ranging from fundamental physics to industrial purposes. Searching for high-performance saturable absorbers (SAs) is vital to the developments of ultrafast fiber lasers. Gold nanorods (GNRs) have been discovered to possess saturable absorption effect.

[Study on optimization of SRAP-PCR reaction system for Pinellia ternata in Suzhou].

Objective: To investigate the optimization system of SRAP-PCR molecular marker technology in the analysis on Pinellia ternata.

Method: SRAP-PCR reaction system for P. ternata was optimized by L16 (5(4)) orthogonal design with five elements (dNTPs, Mg2+, the template DNA, primers, Taq enzyme) and four standards.

[Study on optimization of induction system of test-tube tuberous roots from leaves of Rehmannia glutinosa].

Objective: To study the effect of sucrose and plant growth substances of different concentrations on the induction of test-tube tuberous roots of Rehmannia glutinosa, in order to establish an efficient system for the induction of test-tube tuberous roots from leaves of R. glutinosa.

Method: Leaves from test-tube seedlings of 85-5 R.

[Genetic transformation of Pinellia ternata with Agrobacterium tumefaciens-mediated sHSP genes].

Objective: To establish an efficient genetic transformation system of Pinellia ternata.

Method: With petioles from test-tube seedlings of P. ternata as explants, Agrobacterium tumefaciens mediation method was adopted to explore the effect of phenolic substances, A.

[Effects of shading on photosynthetic characteristics of Pinellia ternata leaves].

Objective: To study the effects of shading on photosynthetic physiology and chlorophyll fluorescence of Pinellia ternata.

Method: Plant growth, chlorophyll content, net photosynthetic rate (P(n)) and chlorophyll fluorescence in P. ternata were investigated under different shading treatments (0%, 70% and 90%) when it grew about 15 cm high.

[Study on bulblet induction of Fritillaria anhuiensis in vitro].

Objective: To study the optimal condition for bulb induction of Fritillaria anhuiensis in vitro.

Method: Effects of sucrose, salicylic acid, active carbon and 5 degrees C pre-incubation on bulb formation in vitro were investigated by adopting the number of bulb and fresh weight as indexes.

Result: The number of bulb and fresh weight in medium added with 50 g x L(-1) sucrose were significantly higher than those with other treatments.

[Establishment and optimization of sliver staining differential display of microtubers from Pinellia ternata in vitro].

Objective: In this study, orthogonal design was used to optinize DDRT-PCR amplification system on Pinellia ternata microtubers in vitro in five factors four levels respectively.

Method: P. ternata stems and microtubers in vitro were selected as explants.

[Primary study on suspension cell culture and polysaccharides content of Achyranthes bidentata].

Objective: To study the relationship between the condition of cell suspension culture and the polysaccharides content in Achyranthes bidentata.

Method: The methods of orthogonal test and alone factor test were used to study the effect of culture time, inoculation concentration, carbon source and pH.

Result And Conclusion: The results showed that the optimum medium to induce polysaccharides in A.

[Application of orthogonal design in tissue culture of Dioscorea opposite].

Objective: To study the effects of different factors on buds and microtuber. These factors included plant growth substances and sucrose.

Method: stems were selected as explants.

[Efficient method for extraction of high quality RNA from microtubers of Pinellia ternata in vitro].

Objective: To extract RNA from Pinellia ternata and lay a foundation for studying the formation mechanism of P. ternata.

Method: By modifying the method recommended by Guanidinium for extracting total RNA from plant tissues rich in phenolic and polysaccharidic compounds, a simple and convenient method for extraction of total RNA from the tubers, stems and leaves of P.

[Change of endogenous hormone around sprout tumble of Pinellia ternata under high temperature stress].

Objective: To study the change of endogenous hormone (ABA, IAA, JA, GA3, ZR) in the leaves, petioles, tubers of Pinellia ternate around sprout tumble. It also provided some valuable information to prevent sprout tumble and increase production.

Method: Tubers of P.

[Study on technique of inducing microrhizome in Zingiber officinale].

Objective: To study the effect of different factors on induction of microtubers in Zingiber officinale. These factors included NAA, PP333, 6-BA and sucrose.

Method: Orthogonal design and plant tissue culture technique were used.

[Effects of salicylic acid on growth of Pinellia ternata].

Objective: To study the effects of salicylic acid of different concentration (SA) on the growth of Pinellia ternate.

Method: SA of different concentration was sprayed to the plant as it grew about 15 cm high. Plant height, total chlorophyll content, activity of SOD, MAD content, photosynthesis speed, intercellular CO2 concentration, the transpiration speed and the leaf temperature were measured after 3 times spray.