Chinese herbal medicines (CHMs) are one of the important bioresources of medicine, which works by unlocking nature's ability to prevent diseases and recover from illnesses. Recently, it has ascended to the world stage and become a global icon. Nowadays, a considerable of researches have focused on the quality evaluation of CHMs.
View Article and Find Full Text PDFCistanches Herba is a medicinal plant that has tonification properties and is commonly used in Asia. Owing to the imbalance between supply and demand, adulterants are frequently added for profit. However, there is no regulatory oversight because quality control tools are not sufficient for identifying heavily processed products.
View Article and Find Full Text PDFThe low electrical conductivity and ordinary lithium-ion transfer capability of LiTiO restrict its application to some degree. In this work, dual-phase LiTiO-TiO (LTOT) was modified by composite zirconates of LiZrO, LiZrO (LZO) to boost the rate capabilities and cyclability. When the homogeneous mixture of LiNO, Zr(NO)·5HO and LTOT was roasted at 700 °C for 5 h, the obtained composite achieved a superior reversible capacity of 183.
View Article and Find Full Text PDFSpecies adulteration in herbal products (HPs) exposes consumers to health risks. Chemical and morphological methods have their own deficiencies when dealing with the detection of species containing the same active compounds in HPs. In this study, we developed a rapid identification method using the recombinase polymerase amplification (RPA) assay to detect two species, Ginkgo biloba and Sophora japonica (as adulteration), in Ginkgo biloba HPs.
View Article and Find Full Text PDFis a fungus that infects Hepialidae caterpillars, mummifying the larvae and producing characteristic fruiting bodies (stromata) that are processed into one of the most valued traditional Chinese medicines (TCM). The product commands a very high price due to a high demand but a very limited supply. Adulteration with other fungi is a common problem and there is a need to test preparation for the presence of the correct fungus.
View Article and Find Full Text PDFRNA-Sequencing (RNA-Seq) is a newly-developed method in transcriptome research, it can afford more accurate transcription information and be more quickly by using Next-generation Sequencing (NGS) technology. RNA-Seq has been widely used in various biological fields. Genuine traditional Chinese medicines (TCM), with good quality and therapeutic effect, were always praised highly and used by famous physicians.
View Article and Find Full Text PDFZhongguo Zhong Yao Za Zhi
October 2014
Genuine medicinal materials with special characteristics of Traditional Chinese Medicine (TCM), is recognized as high quality medicine. Both ancient records and modern research considered that the origin is an important reason for the formation of genuine medicinal materials. However, blindly transplanting of genuine medicinal materials has led to the quality decline and counterfeit medicines appeared in production or sale progress, which may increase the risk of accidents in TCM.
View Article and Find Full Text PDFComput Intell Neurosci
October 2015
This paper proposes an indicator of seismic performance based on life-cycle cost of a building. It is expressed as a ratio of lifetime damage loss to life-cycle cost and determines the seismic performance of isolated buildings. Major factors are considered, including uncertainty in hazard demand and structural capacity, initial costs, and expected loss during earthquakes.
View Article and Find Full Text PDFZhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
June 2013
Human babesiosis, a malaria-like zoonosis transmitted by the tick, is mainly distributed in Europe, USA and some Asian countries. There are various kinds of diagnostic methods for babesiosis caused by Babesia microti, but many of them are still in the preliminary stage. This article reviews the main diagnostic techniques and the existing problems.
View Article and Find Full Text PDFTo identify Salvia shandongensis and its relatives at molecular level, the psbA-trnH intergenic region of three species including Salvia shandongensis, Salvia miltiorrhiza and S. miltiorrhiza f. alba were amplified and sequenced.
View Article and Find Full Text PDFUnlabelled: The DNA barcoding method was used to accurately and rapidly identify Corni Fructus and its adulterants.
Methods: Genomic DNA extracted from Corni Fructus and its adulterants were used as templates. The ITS (internal trascribed spacer) regions were amplified using polymerase chain reaction.
Zhongguo Zhong Yao Za Zhi
January 2013
Since the research of molecular identification of Chinese Materia Medica (CMM) using DNA barcode is rapidly developing and popularizing, the principle of this method is approved to be listed in the Supplement of the Pharmacopoeia of the People's Republic of China. Based on the study on comprehensive samples, the DNA barcoding systems have been established to identify CMM, i.e.
View Article and Find Full Text PDFIn this study, Notopterygii Rhizoma et Radix was used to verify the stability and accuracy of DNA barcodes in identification of Chinese materia medica for the first time. All genomic DNAs from thirty one samples were extracted. The ITS (internal transcribed spacer) regions were amplified and sequenced bi-directionally.
View Article and Find Full Text PDFTo identify the original plant of Daturae Flos from its adulterants by DNA barcoding, the sequences of ITS2, psbA-trnH, matK, rbcL of four species including Datura metel, Darura innoxia, Darura stramonium and Brugmansia arborea were compared and analyzed. The PCR and sequencing success rate of the four regions (ITS2, psbA-trnH, matK, rbcL) was 100%, 90%, 100% and 85%, respectively. Sequences were assembled with CodonCode Aligner.
View Article and Find Full Text PDFThe dried succulent stems of Cistanche (Cistanche deserticola Y. C. Ma and Cistanche tubulosa Wight.
View Article and Find Full Text PDFDNA barcoding is a novel technology that uses a standard DNA sequence to facilitate species identification. Although a consensus has not been reached regarding which DNA sequences can be used as the best plant barcodes, the psbA-trnH spacer region has been tested extensively in recent years. In this study, we hypothesize that the psbA-trnH spacer regions are also effective barcodes for Dendrobium species.
View Article and Find Full Text PDFA liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated for the determination and pharmacokinetics of danshensu in rat plasma samples using ferulic acid as internal standard (IS). The plasma samples were treated by liquid-liquid extraction, and the analyses were determined using electrospray negative ionization mass spectrometry in selected reaction monitoring (SRM) mode. The signal intensity of the m/z 196.
View Article and Find Full Text PDFZhongguo Zhong Yao Za Zhi
January 2005
Objective: To study the relationship between plant growth and accumulation of active ingredients in Salvia miltiorrhiza.
Method: Transplants of S. miltiorrhiza were sampled at 20 day intervals.
Objective: To confirm the amount of fertilizer and the ration of fertilizer.
Method: Through the experiment of planting Salvia miltiorrhiza in pot and in field were carried out.
Result: When N:P = 1:1, The production was 1.