Analysis of nicotine-induced metabolic changes in Blakeslea trispora by GC-MS.

Blakeslea trispora is a natural source of carotenoids, including β-carotene and lycopene, which have industrial applications. Therefore, classical selective breeding techniques have been applied to generate strains with increased productivity, and microencapsulated β-carotene preparation has been used in food industry (Li et al., 2019).

Comparative Metabolic Responses Induced by Pyridine and Imidazole in .

Lycopene cyclase needs to be inhibited by the blockers like pyridine or imidazole in the lycopene accumulation of . This work investigated how pyridine and imidazole impacted the basal metabolism of , the results helped us understand how they could affect the lycopene production and application, and see the metabolic risks from different inhibitors. In this study, the highest yield of lycopene with pyridine was obtained at 176 mg/L without amino acids supplement, and got more lycopene at 237 mg/L adding tyrosine, lysine, proline all together as 0.

Microencapsulated β-carotene preparation using different drying treatments.

β-Carotene is one of the most abundant natural pigments in foods; however, usage of β-carotene is limited because of its instability. Microencapsulation techniques are usually applied to protect microencapsulated β-carotene from oxidization. In this study, β-carotene was microencapsulated using different drying processes: spray-drying, spray freeze-drying, coating, and spray granulation.

Genome shuffling and ribosome engineering of Streptomyces virginiae for improved virginiamycin production.

The production of virginiamycin (VGM) from Streptomyces virginiae was improved by genome shuffling and ribosome engineering companied with a high-throughput screening method integrating deep-well cultivation and the cylinder-plate detecting. First, a novel high-throughput method was developed to rapidly screen large numbers of VGM-producing mutants. Then, the starting population of genome shuffling was obtained through ultraviolet (UV) and microwave mutagenesis, and four mutants with higher productivity of VGM were selected for genome shuffling.

Bipedicle orbicularis oculi flap in the reconstruction of the lower eyelid ectropion.

Background: Lower eyelid ectropion is conventionally reconstructed with a local flap or full-thickness skin graft. However, scar contracture and recurrence of ectropion often occur. This article describes an effective surgical technique for lower eyelid ectropion repair using a bipedicle orbicularis oculi muscle or myocutaneous flap from the upper eyelid.

A novel approach for improving the productivity of ubiquinone-10 producing strain by low-energy ion beam irradiation.

Agrobacterium tumefaciens ATCC4452 cells were irradiated by nitrogen ion beam, a new mutagen, with energy of 10 keV and fluence ranging from 2.6x10(14) ions/cm2 to 6.5x10(15) ions/cm2.

Studies on Etching and Damage Action of Low Energy Ion Beam on icrobial Cells.

The action of 20 keV N(+) ion on Deinococcus radiodurans and E. coli was investigated by means of scanning electron microscope(SEM) and electron spin resonance(ESR). The results showed that ion implantation exerted direct etching damage and indirect free radicals action by energy deposition on the cells of the two microbes.