Proposing a novel molecular subtyping scheme for predicting distant recurrence-free survival in breast cancer post-neoadjuvant chemotherapy with close correlation to metabolism and senescence.

Background: High relapse rates remain a clinical challenge in the management of breast cancer (BC), with distant recurrence being a major driver of patient deterioration. To optimize the surveillance regimen for distant recurrence after neoadjuvant chemotherapy (NAC), we conducted a comprehensive analysis using bioinformatics and machine learning approaches.

Materials And Methods: Microarray data were retrieved from the GEO database, and differential expression analysis was performed with the R package 'Limma'.

Application of symptom-based mind mapping combined with PBL teaching method in emergency trauma standardized resident training in MDT model.

Explore the feasibility and effectiveness of accepting mind mapping combined with problem-based learning (PBL) teaching method in the standardized training of emergency surgery residents in the multi-disciplinary team (MDT) model of emergency trauma. Eighty-nine doctors under training who rotated in the Department of Emergency Surgery of the First Affiliated Hospital of Anhui Medical University from January 2021 to January 2022 were selected as the study subjects, and randomly divided into a group receiving mind mapping combined with PBL teaching and a group receiving traditional lecture-based learning teaching. Mini-clinical evaluation exercise (Mini-CEX), direct observation of procedural skills (DOPS), teaching adherence, and satisfaction assessments were completed at the time of discharge from the department.

Preimplantation genetic testing for aneuploidy in severe male factor infertility: protocol for a multicenter randomised controlled trial.

Introduction: Conventional intracytoplasmic sperm injection (ICSI) is a widely used treatment for couples with severe male infertility. However, there are controversies regarding the selection and the damage to gametes during the ICSI procedure. Although preimplantation genetic testing for aneuploidies (PGT-A) can give genetic information about embryos for transfer and improve fertility rate, and it is widely used in women with recurrent spontaneous abortion or advanced age, PGT-A is not only more expensive but also has unclear effectiveness with respect to the improvement of fertility rate among couples with severe male infertility.

The Impact of Gestational Weight Gain on the Risks of Adverse Maternal and Infant Outcomes among Normal BMI Women with High Triglyceride Levels during Early Pregnancy.

A high maternal triglyceride (mTG) level during early pregnancy is linked to adverse pregnancy outcomes, but the use of specific interventions has been met with limited success. A retrospective cohort study was designed to investigate the impact of gestational weight gain (GWG) on the relationship between high levels of mTG and adverse pregnancy outcomes in normal early pregnancy body mass index (BMI) women. The patients included 39,665 women with normal BMI who had a singleton pregnancy and underwent serum lipids screening during early pregnancy.

[Meta-analysis of the Effects of Metal Mining on Soil Heavy Metal Concentrations in Southwest China].

Metal mining is one of the main contributors of soil heavy metals. Previous studies examining the impact of metal mining on surrounding soil have mainly focused on one or a few metal mining areas. However, such studies cannot effectively inform the management of heavy metal pollution in soil at an inter-provincial scale.

[Preparation and evaluation of four kinds of mixed essential oil liposomes in Jieyu Anshen Formula].

In order to increase the stability and solubility of essential oil in Jieyu Anshen Formula, this study was to prepare the essential oil into liposomes. In this experiment, the method for the determination of encapsulation efficiency of liposomes was established by ultraviolet spectrophotometer and dextran gel column. The encapsulation efficiency and particle size of liposomes were used as evaluation indexes for single factor investigation and Box-Behnken design-response surface method was used to optimize the design.

The Ankyrin Repeat Domain 49 (ANKRD49) Augments Autophagy of Serum-Starved GC-1 Cells through the NF-κB Pathway.

The ankyrin repeat domain 49 (ANKRD49) is an evolutionarily conserved protein highly expressed in testes. However, the function of ANKRD49 in spermatogenesis is unknown. In this study, we found that ANKRD49 resides primarily in nucleus of spermatogonia, spermatocytes and round spermatids.

Talin-1 correlates with reduced invasion and migration in human hepatocellular carcinoma cells.

Background: Talin-1 is a cytoskeleton protein that participates in cell migration and plays a role in tumor formation, migration, and metastasis in different types of cancer. Chinese investigators have observed that the levels of Talin-1 protein and mRNA expression in HCC tissues are significantly lower than in the adjacent non-cancerous tissue. However, Japanese investigators have reported that Talin-1 is upregulated in HCC.

Facial reanimation by one-stage microneurovascular free abductor hallucis muscle transplantation: personal experience and long-term outcomes.

Background: In 1990, Jiang Hua introduced a new method using one-stage reconstruction with free abductor hallucis muscle transfer for dynamic reanimation of established unilateral facial paralysis. The authors present their experience with this procedure and analyze the postoperative complications and long-term functional and aesthetic outcomes.

Methods: From March of 1990 to March of 2010, 45 patients underwent the free abductor hallucis muscle transfer procedure in the authors' department.

Classic citations in main plastic and reconstructive surgery journals.

Background: The number of citations of an article in scientific journals reflects its impact on a biomedical specialty and its recognition in the scientific community. In this study, we identified and analyzed the 100 most highly cited articles published between 1971 and 2011 in journals pertaining to plastic and reconstructive surgery research.

Methods: We selected 6 high-impact journals for literature search in the database of the Science Citation Index (1971 to 2011).

Two methods can simultaneously display both intramuscular nerves and blood vessels.

Background: The arrangement and relationship of intramuscular nerves and blood vessels are critical to clinical physicians, but the majority of previous studies could not reflect them precisely.

Methods: In method 1, after rabbits were perfused with barium sulfate liquid silica gel, the specimen muscle was isolated and subjected to Sihler staining. The specimen muscles then underwent optical photography and molybdenum target radiography.

National representation in the plastic and reconstructive surgery literature: a bibliometric analysis of highly cited journals.

Background: In recent years, significant growth has been observed in the field of plastic and reconstructive surgery. However, the national productivity to the field of plastic and reconstructive surgery remains unknown. We therefore intended to reveal national contributions in the field of plastic and reconstructive surgery.

Growing trend of China's contribution to the field of plastic and reconstructive surgery: a 10-year study of the literature.

Background: In the past decade, plastic and reconstructive surgery in China has achieved great advances. However, the scientific publications in plastic and reconstructive surgery in the major regions of China-Mainland, Hong Kong, and Taiwan are unknown. We therefore intended to reveal the contribution of articles from Chinese authors to the field of plastic and reconstructive surgery.

Talin1, a valuable marker for diagnosis and prognostic assessment of human hepatocelluar carcinomas.

Background/aims: Hepatocellular carcinoma (HCC) is one of the most lethal and prevalent cancers in the human population. Despite its significance, there is only limited understanding of pathological mechanisms and therapeutic options. Talin1, a focal adhesion complex protein that is required for cell adhesion and motility, regulates integrin interactions with extracellular matrix (ECM).

[Experimental research on anti-respiratory syncytial virus effect in vitro of earthworm coelomic fluid].

Objective: To explore the antivirus function in vitro of earthworm coelomic fluid (ECF) by researching its effect on inhibiting respiratory syncytial virus (RSV).

Methods: By the method of Hep-2 cell culture and using ribavirin as a positive control, the anti-RSV effect of ECF was investigated by observing cytopathic effect (CPE) with MTT colorimetric assay.

Results: In Hep-2 cells, the CC50 of ECF and ribavirin were 3.

Occurrence of brackish water phytoplankton species at a closed coral reef in Nansha Islands, South China Sea.

The phytoplankton assemblage of Zhubi Reef, a closed coral reef in Nansha Islands (South China Sea, SCS) was studied in June 2007. A total of 92 species belonging to 53 genera and four phyla have been identified. The dominant taxa in the lagoon were the diatom Chaetoceros and cyanobacteria Nostoc and Microcystis, while in reef flats were cyanobacteria Trichodesmium erythraeum, dinoflagellates Gymnodinium and Prorocentrum.

Hyperbaric oxygen preconditioning reduces the incidence of decompression sickness in rats via nitric oxide.

Divers are at risk of decompression sickness (DCS) when the ambient pressure decrease exceeds a critical threshold. Hyperbaric oxygen (HBO2) preconditioning has been used to prevent various injuries, but the protective effect on DCS has not been well explored. To investigate the prophylactic effect of HBO2 on DCS, rats were pretreated with HBO2 (250 kPa-60 minutes) (all the pressures described here are absolute pressure) for 18 hours before a simulated air dive (700 kPa-100 minutes) with fast decompression to the surface at the rate of 200 kPa/min (n=33).

[Optimization of human anti-HBsAg scFv secretary expression in Escherichia coli].

Objective: To explore the conditions for high expression of anti-HBsAg scFv A-15 in E. coli, increase the production of the scFv in the culture medium.

Methods: By changing induction occasion, concentration of inductor IPTG and induction time, influence of various conditions on expression of anti-HBsAg scFv A-15 was analyzed through ELISA.

[Role of integrin-linked kinase in renal tubular epithelial-mesenchymal transition and the regulatory effect of urokinase on its expression in mice with obstructive nephropathy].

Objective: To investigate the role of integrin-linked kinase (ILK) on renal tubular epithelial-mesenchymal transition and the regulatory effect of urokinase on LIK expression in mice with obstructive nephropathy.

Methods: Normal male mice were randomly divided into sham-operated group (n=20), unilateral ureteral obstruction (UUO) group (n=28), and UUO with urokinase treatment group (uPA, n=28), and UUO was induced surgically in the latter two groups. The mice were sacrificed on days l, 3, 7 and 14 after the surgery, and renal interstitial fibrosis (RIF) was graded according to the result of Masson staining.