[Effects of a novel spermine oxidase inhibitor SI-4650 on proliferation and EMT of human ovarian cancer SKVO-3 cells].

To investigate the effects of SI-4650, a novel small molecule inhibitor of spermine oxidase (SMO), on the proliferation and epithelial mesenchymal transformation (EMT) of human ovarian cancer SKVO-3 cells as well as its underlying molecular mechanisms. SKVO-3 cells treated with 0 μmol/L SI-4650 were used as control group, SKVO-3 cells treated with 30, 60 μmol/L SI-4650 were used as experimental group. The effects of SI-4650 on the activity of SMO, the polyamine contents and the cellular reactive oxygen species (ROS) were detected.

[Distribution Characteristics of Microplastics in Ice Sheets and Its Response to Salinity and Chlorophyll a in the Lake Wuliangsuhai].

There are sparse reports on the distribution of microplastics in the ice sheets of freshwater lakes. In this study, the abundance, color, shape, and species of microplastics in the ice sheet of Lake Wuliangsuhai were characterized using field sampling and microscope observations. Fourier-transform infrared spectroscopy (FTIR) and correlation analysis were used to examine the distribution of microplastics contained in the lake and its relationships with salinity and chlorophyll a.

Nanopore Formation and Structural Changes in Black Shale During the Initial Weathering Stage: A Longmaxi Formation Profile in Northwestern Hunan, China.

Understanding the controls on composition changes and porosity evolution in the critical zone of shale remains a major challenge. The aim of the present study is to develop a model of the changes in mineral compositions, chemical compositions and nanopore formation in shale during the initial weathering stage. To understand these processes, we selected a Silurian shale profile rich in pyrite and organic matter located in South China.

[Effects of a novel polyamine metabolic enzyme inhibitor SI-4650 on proliferation of colon cancer CT-26 cells and its mechanism].

Objective: To investigate the effects of a novel polyamine metabolism enzyme inhibitor SI-4650 on autophagy and apoptosis of colon cancer CT-26 cells as well as their correlation.

Methods: CT-26 cells treated with 40, 80 μmol·L SI-4650 alone or in combination with 3-MA were used as experimental group. CT-26 cells treated with 0 μmol·L SI-4650 alone or in combination with 3-MA were used as control group.

[Experimental study on improvement of alcoholic liver disease by polysaccharides from Balanophora henryi through targeting miR-122a].

The aim of this paper was to investigate the effect of total polysaccharide from Balanophora henryi(TBP) on alcoholic liver disease(ALD) and explore the possible mechanism. C57 BL/6 N mice were randomly divided into 4 groups: pair-feeding group, alcohol-feeding model group, model+TBP group and TBP drug control group. The Gao-binge method was used to prepare the chronic ALD model, and at the same time, 400 mg·kg~(-1) TBP was given for interventional therapy.

[Occurrence Characteristics and Quality Estimation of Microplastics in Drainage Ditches in Hetao Irrigation District of Inner Mongolia].

Microplastic pollution due to land runoff has gained increasing attention as it is closely associated with human beings. In this study, we analyzed the occurrence characteristics of microplastics in drainage channel and main drainage channel in Hetao irrigation district of Inner Mongolia and estimated its quality. Through field sampling, the density separation of suspension method and microscope observation, Fourier infrared spectrum measurement, and proportional flow method, the abundance distribution, shape, color, particle size, and chemical composition of microplastics in the water body and sediment of the drainage channel and main drainage channel in the Hetao irrigation district were identified.

Apoptotic Melanoma B16-F1 Cells Induced by Lidamycin Could Initiate the Antitumor Immune Response in BABL/c Mice.

In the process of tumor cell apoptosis induced by specific regents, calreticulin (CRT) was transferred from endoplasmic reticulum (ER) onto the cell membrane. These tumor cells, when used as the cellular vaccine to immunize experimental animals, could initiate effective antitumor immunoresponse against homologous tumor cells. This is referred to as immunogenic cell death.

Polyamine analog TBP inhibits proliferation of human K562 chronic myelogenous leukemia cells by induced apoptosis.

The aim of the present study was to investigate the effects of the novel polyamine analog tetrabutyl propanediamine (TBP) on the growth of K562 chronic myelogenous leukemia (CML) cells and the underlying mechanism of these effects. MTT was used for the analysis of cell proliferation and flow cytometry was performed to analyze cell cycle distribution. DNA fragmentation analysis and Annexin V/propidium iodide double staining were used to identify apoptotic cells.

Vascularised greater trochanter bone graft, combined free iliac flap and impaction bone grafting for osteonecrosis of the femoral head.

Purpose: To investigate the curative efficacy of osteonecrosis of the femoral head (ONFH) in a hip-preserving operative approach, by grafting a vascularized greater trochanter flap combined with a free iliac flap, in an attempt to seek an innovative approach for patients who suffered middle to late stage ONFH without total hip arthroplasty (THA) surgery.

Method: Our research included a total of 60 patients (66 hips) who accepted hip-preserving surgery by grafting a vascularized greater trochanter flap combined with a free iliac flap which was tightly filled by hammering because of ONFH (most were Association Research Circulation Osseous (ARCO) stage III patients) from January, 2006 to December, 2010. A Harris Hip Score was obtained during follow-ups, evaluating the clinical efficacy, X-rays were taken regularly for image assessing, and the SF-36 scale was used for estimating quality of life.

[Preparation and identification of monoclonal antibody against human spermine oxidase].

Aim: To prepare mouse anti-human spermine oxidase (anti-hSMO) monoclonal antibody (mAb) and testify its application in the biological techniques including Western blotting and immunohistochemistry.

Methods: Plasmid pET-15b/SMO was first transferred into BL21 (DE3), and then SMO recombinant protein with 6×His tag was induced to express by IPTG and purified by Ni-NTA resin. The purified recombinant SMO was used to immunize BALB/c mouse.

[Expression and functional analysis of CRT-E2-EGFP fusion protein in B16 cells].

Aim: To detect the expression of the Calreticulin and HPV E2 Fusion Protein in B16, and study the effects on proliferation and apoptosis of B16 cell lines in vivo.

Methods: To construct eukaryotic fluoresce expression vector pEGFP-CRT-E2, pEGFP-CRT and pEGFP-E2. Then the recombinant plasmids were transfected into B16 cells by Lipofectamine 2000.

Influence of HBcAg in liver cell plasma on expression of transforming growth factor-beta 1 in liver tissue of low-grade chronic hepatitis B patients.

Aim: To study the influence of HBcAg on the expression of transforming growth factor-beta 1 (TGF-beta1) in liver tissue of low-grade chronic hepatitis B (CHB) patients.

Methods: The expression of TGF-beta1 and HBcAg in liver samples from 93 low-grade CHB patients was detected by immunohistochemistry and valuated by semi-quantitative scoring.

Results: In the 93 low-grade CHB patients, HBcAg was expressed in cell plasma but not in the liver tissue.

[The relationship between acute rejection and expression of sCD30 for the patients after kidney transplantation].

Objective: To study the relationship between the sCD30 and acute rejection.

Methods: We tested the sCD30 level in serum for 58 cases with kidney transplantation before and the 7th day and 28th day after operation by ELISA. 31 healthy individual for control group, and simultaneously recorded the incidence of rejection after kidney transplantation.