Cloning, Overexpression, and Characterization of a Thermostable, Organic Solvent-Tolerant Laccase from ARA and Its Application to Dye Decolorization.

A thermostable and organic solvent-tolerant bacterial laccase from ARA has been expressed heterologously and characterized, which shows potential decolorization capacity to various types of industrial synthetic dyes. The optimal temperature and pH were 85 °C and 3.5, respectively, while the purified recombinant laccase B.

[Comparison of three different thermophilic beta-glucosidases for baicalin transformation].

Three different beta-glycosidase sequences of Ttebgl3, Tpebgl1 and Tpengl3 from Thermotoga thermarum DSM 5069 and Thermotoga petrophila RKU-1 were analyzed. Also, the influence of temperature, pH, concentration of DMSO, metal ions and kinetic constant on catalytic conversion of baicalin had been compared. The results indicated that the optimal pH and optimum temperature for transformation of baicalin was 4.

RNA-Seq analysis and comparison of the enzymes involved in ionone synthesis of three cultivars of Osmanthus.

To comprehend the molecular mechanisms that control the differences in the composition of Osmanthus essential oils, the RNA-seq data and differentially expressed genes in different cultivar Osmanthus were studied. cDNA libraries of "jinqiugui," "baijie," and "rixianggui" were sequenced using Illumina HiSeq 2000. All of the enzymes involved in ionone synthesis were verified.

[Preparation of baicalein using thermophilic and sugar-tolerant beta-glucosidase].

The reaction conditions of baicalin hydrolyzed into baicalein by a kind of thermophilic and sugar-tolerant beta-glucosidase were studied in this paper. The beta-glucosidase could catalyze baicalin into baicalein well in the acetic acid-sodium acetate buffer. The optimal enzyme activity was at 85 degrees C and pH 5.

[The value of CT in assessing the prognosis of sever acute pancreatitis: a report of 62 cases].

Objective: To study the relationship between CT imaging classification criteria and the prognosis of severe acute pancreatitis (SAP).

Methods: From January 2000 to December 2009, 62 cases with SAP were analyzed, retrospectively. They were all executed CT examination in 72 h after admission, and their CT imaging were classified as grade C (n = 13), grade D (n = 26), and grade E (n = 23) according to the Balthazar classification criteria and also classified as grade I (n = 11), grade II (n = 39), grade III (n = 12) according to the Balthazar CT severity index (CTSI) criteria, respectively.