The association of CYP2C9 gene polymorphisms with colorectal carcinoma in Han Chinese.

Background: Cytochrome P450 (CYP) 2C9 is an important enzyme involved in xenobiotics metabolism. This study investigated the association of CYP2C9 gene coding region polymorphisms with colorectal cancer (CRC) in Chinese Han population.

Methods: Four hundred and eighty-three healthy controls and 286 sporadic CRC patients participated in this study.

Cloning, Sequencing and Preliminary Expression of Human RP2 Gene.

RP2 is an X-linked retinitis pigmentosa gene, which was newly discovered by positional cloning. A polymerase chain reaction (PCR) was conducted to screen a full-length cDNA fragment, defined as hRP2a, which included the coding region of hRP2, in a human retina cDNA library. HRP2a gene was cloned into the pJLA503 vector and hRP2 gene was subcloned into the expression vector pP(RO)EX HTa.

Construction and Physical Mapping of the 35 cM YAC Contigs for the Region of Xp11.2 - p21.3.

One hundred and fifty-one YACs were selected from three libraries with a series of STS and other DNA probes of the human X chromosome p11.2 - p21.3 region.

A 4.3 Mb YAC Contig in Human Xp11.2: Long-Range Restriction Mapping and Identification of CpG Islands.

The Xp11.2 region o the human X chromosome contains genes involved in a number of inherited diseased, with at least one locus that escapes X chromosome inactivation, as well as abnormal methylation polymorphism. We isolated a series of yeast artificial chromosome (YAC) clones by hybridization screening with DNA probes localized within this region and assembled them into a 4.