Genome-wide analysis of long noncoding RNA profiles in pseudorabies-virus-infected PK15 cells.

Recently, an increasing number of studies have shown that long noncoding RNAs (lncRNAs) are involved in host metabolism after infection with pseudorabies virus (PRV). In our study, via RNA sequencing analysis, a total of 418 mRNAs, 137 annotated lncRNAs, and 312 new lncRNAs were found to be differentially expressed. These lncRNAs were closely associated with metabolic regulation and immunity-related signalling pathways, including the T-cell receptor signalling pathway, chemokine signalling pathway, mitogen-activated protein kinase (MAPK) signalling pathway, TNF signalling pathway, Ras signalling pathway, calcium signalling pathway, and phosphatidylinositol signalling system.

Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells.

The nonstructural protein 10 (nsp10) of porcine reproductive and respiratory syndrome virus (PRRSV) encodes for helicase which plays a vital role in viral replication. In the present study, a truncated form of nsp10, termed nsp10a, was found in PRRSV-infected cells and the production of nsp10a was strain-specific. Mass spectrometric analysis and deletion mutagenesis indicated that nsp10a may be short of about 70 amino acids in the N terminus of nsp10.

Equine infectious anemia virus in China.

Equine infectious anemia is an equine disease caused by equine infectious anemia virus, which was first reported in 1840. Equine infectious anemia virus research in China started in the 1960s, focusing on etiology, pathology, diagnosis, and immunology. Notably, in 1978 an attenuated vaccine was successfully developed for equine infectious anemia virus, effectively preventing equine infectious anemia virus in China.

A new recombined porcine reproductive and respiratory syndrome virus virulent strain in China.

Porcine reproductive and respiratory syndrome (PRRS) is one of the most important swine diseases worldwide. In the present study, a new virulent strain of PRRS virus (PRRSV), GDsg, was isolated in Guangdong province, China, and caused high fever, high morbidity, and high mortality in sows and piglets. The genome of this new strain was 15,413 nucleotides (nt) long, and comparative analysis revealed that GDsg shared 82.

Profiling and Identification of Novel Immunogenic Proteins of Staphylococcus hyicus ZC-4 by Immunoproteomic Assay.

Staphylococcus hyicus has caused great losses in the swine industry by inducing piglet exudative epidermitis (EE), sow mastitis, metritis, and other diseases and is a threat to human health. The pathogenesis of EE, sow mastitis, and metritis involves the interaction between the host and virulent protein factors of S. hyicus, however, the proteins that interact with the host, especially the host immune system, are unclear.

[Relationship between CO II gene of mtDNA of Lucilia sericata and latitude interval].

Objective: To deduce the region that the geographical species of Lucilia sericata come from and determine the scene of crime (SOC) based on the gene analysis of mtDNA CO II.

Methods: A 635 bp region for CO II of 4 Lucilia sericata (belong to 2 geographical species) were collected and sequenced, compared with the data of GenBank. A neighbour-joining tree with the Tamura and Nei model was constructed by MEGA2.

[Forensic applications of the sequencing of mitochondrial DNA cytochrome oxidase subunit I gene for sarcosaphagous flies (Diptera) in Huhhot and Dunhuang district].

Objective: To solve the problems of identification of Sarcosaphagous flies and their larvae, pupas and eggs.

Methods: Sarcosaphagous Flies (Diptera) Samples were collected on the corpses of rabbits in the Huhhot district and a pig in the Dunhuang district. A 278bp region in the cytochrome oxidase subunit I (CO I) gene in mtDNA was analysed by DNA sequencing, A neighbour-joining tree using the Tamura and Nei model of nucleotide substitution was also constructed using the MEGA2.

[The study of the major sarcosaphagous flies in Hohhot].

Objective: In order to determine the major species of sarcosaphagous flies and their regular activity on carcass in Hohhot district.

Methods: Six rabbits were killed and placed outdoors at different time from July to October in Hohhot district. Some species of sarcosaphagous flies that appeared the cadavers were observed and identified.

[A study of quadriplex PCR with chimeric primers for short tandem repeats loci].

Objective: To establish a simplified method of multiplex PCR based on chimeric primers for STR loci.

Methods: A set of chimeric primers and universal primers were designed to carry out a multiplex PCR for amplifying four short tandem repeats(STR) loci. Then the amplified STR loci were detected by polyacrylamide gel electrophoresis and the gels were sliverstained.