Molecular cloning, prokaryotic expression and its application potential evaluation of interferon (IFN)-ω of forest musk deer.

Forest musk deer (Moschus berezovskii) are currently a threatened species under conservation, and the development of captive populations is restricted by health problems. To evaluate the application potential of interferon (IFN)-ω in the prevention and control of forest musk deer disease, 5 forest musk deer IFN-ω (fmdIFNω) gene sequences were successfully obtained by homologous cloning method for the first time. FmdIFNω5 was selected and recombinant fmdIFNω protein (rIFNω) was successfully expressed by pGEX-6P-1 plasmid and E.

Preliminary study on the role of novel LysR family gene kp05372 in Klebsiella pneumoniae of forest musk deer.

LysR-type transcriptional regulators are involved in the regulation of numerous cellular metabolic processes in Klebsiella pneumoniae, leading to severe infection. Earlier, we found a novel LysR family gene, named kp05372, in a strain of K. pneumoniae (designated GPKP) isolated from forest musk deer.

Affinity of Streptococcal G Protein to Forest Musk Deer () Serum Immunoglobulin G.

Forest musk deer (FMD; ) immunoglobulin G efficiently bound to streptococcal G protein (SPG) and weakly bound to staphylococcal A protein. The results suggested that horseradish peroxidase-conjugated SPG could be chosen as an enzyme-labeled antibody substitute and laid a foundation for immunologic research in FMD disease.

Fine-Tuning the Porosities of the Entangled Isostructural Zn(II)-Based Metal-Organic Frameworks with Active Sites by Introducing Different N-Auxiliary Ligands: Selective Gas Sorption and Efficient CO Conversion.

Three new pairs of 2-fold interpenetrated and self-entangled three-dimensional isostructural porous metal-organic frameworks (MOFs), [Zn(L1)(x)]·0.5HO (x = bipy for , bpa for , and bpe for ) and [Zn(L2)(x)]·0.5HO (x = bipy for , bpa for , and bpe for ) [bipy = 4,4'-bipyridine, bpa = 1,2-bis(4-pyridyl)ethylene, and bpe = 1,2-bis(4-pyridyl)ethylene], have been created and fine-tuned via similar skeleton ligands 2-(imidazol-1-yl)terephthalic acid (HL1) and 2-(1-1,2,4-triazol-1-yl)terephthalic acid (HL2) and N-auxiliary coligands with different linking groups.

Identification and pathogenicity analysis of Streptococcus equinus FMD1, a beta-hemolytic strain isolated from forest musk deer lung.

Streptococcus spp. cause a wide range of diseases in animals and humans. A Streptococcus strain (FMD1) was isolated from forest musk deer lung.

Effects of compound probiotics on the weight, immunity performance and fecal microbiota of forest musk deer.

Probiotics are intended to provide health benefits when consumed, generally by improving or restoring the gut flora. The health problems of forest musk deer (FMD, Moschus berezovskii), a threatened species currently under conservation, restrict the development of captive musk deer. This study was conducted with the aim of analyzing the effects of forest musk deer compound probiotics (FMDPs) on weight, immunity performance and fecal microbiota in FMD by measuring average daily weight gain (ADG) and immune-related factors and by using high-throughput 16S rRNA sequencing to investigate differences in the fecal microbiota among the control group (4 samples), treatment group A (4 samples) and treatment group B (4 samples).

A new stable luminescent Cd(ii) metal-organic framework with fluorescent sensing and selective dye adsorption properties.

A new luminescent metal-organic framework, {[Cd3(HL)2(H2O)3]·3H2O·2CH3CN}n (1) (H4L = 1-(3,5-dicarboxylatobenzyl)-3,5-pyrazole dicarboxylic acid), has been synthesized by solvothermal reaction and characterized by infrared spectroscopy, powder X-ray diffraction, thermogravimetry measurements and so on. 1 shows a new trinodal (4,4,6)-connected topology. Importantly, 1 displays intense luminescence in the solid state and high luminescent sensitivity and selectivity for Fe3+, CrO42- and Cr2O72- ions in aqueous solution, making it a potential probe for detecting these substances.

ISOLATION, IDENTIFICATION, AND GENOME ANALYSIS OF LUNG PATHOGENIC KLEBSIELLA PNEUMONIAE (LPKP) IN FOREST MUSK DEER.

Klebsiella pneumoniae is an important pathogen commonly associated with opportunistic infections. In this study, lung pathogenic K. pneumoniae (LPKP) was isolated and identified from suppurative pneumoniae in forest musk deer by conventional methods and by 16S ribosomal RNA sequence analysis.

CHARACTERIZATION OF VIRULENCE GENES AND ANTIMICROBIAL RESISTANCE OF LUNG PATHOGENIC ESCHERICHIA COLI ISOLATES IN FOREST MUSK DEER (MOSCHUS BEREZOVSKII).

This study investigated genotypic diversity, 26 virulence genes, and antimicrobial susceptibility of lung pathogenic Escherichia coli (LPEC) isolated from forest musk deer. Associations between virulence factors (VFs) and phylogenetic group, between antimicrobial resistance (AMR) and phylogenetic group, and between AMR and VFs were subsequently assessed. The results showed 30 LPEC isolated were grouped into seven different clusters (A, B, C, D, E, F, and G).

[Genetic polymorphism of 15 STR loci in Tibetan population].

The polymorphism distributions of 15 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818, and FGA) were investigated in a Tibetan population by multiplex PCR amplification using five fluorochromes (6FAM, VIC, NED, PET, LIZ). Gene frequency, discrimination power (DP), heterozygosity (H), polymorphism information content (PIC) and probability of paternity exclusion (EPP) were calculated, and all loci were tested for Hardy-Weinberg equilibrium. Results indicate that the gene frequency of these 15 STR loci is in Hardy-Weinberg equilibrium.