A Novel Nitrite Reductase from for Efficient Degradation of Nitrite.

Nitrite reductases play a crucial role in the nitrogen cycle, demonstrating significant potential for applications in the food industry and environmental remediation, particularly for nitrite degradation and detection. In this study, we identified a novel nitrite reductase (NiR) from a newly isolated denitrifying bacterium, YD01. We constructed a heterologous expression system using BL21/pET28a-Nir, which exhibited remarkable nitrite reductase enzyme activity of 29 U/mL in the culture broth, substantially higher than that reported for other strains.

[Design and application of guide device for femoral neck section].

Objective: To explore the application effect of a new guide device designed according to the anatomical characteristics of the femoral neck cross section to assist the internal fixation of multiple screw of the femoral neck.

Methods: From October to December 2016, 10 adult dry femur specimens, including 7 males and 3 females, aged 37 to 58(47.5±7.

[Discerning the femoral neck anteversion (FNA) from the torsion angle on 3D CT].

Objective: To discern the differences between femoral neck anteversion (FNA) and torsion angle through 3D CT reconstruction.

Methods: From March 2010 to October 2010,30 healthy adult volunteers' femur were reconstructed by 3D CT, included 15 males and 15 females with an average age of (43.66 +/- 7.

Expression of Yes-associated protein in non-small cell lung cancer and its relationship with clinical pathological factors.

Background: Yes-associated protein (YAP) plays an important role in signal transduction and gene transcription regulation in normal cells, with elevated and over-expressed YAP levels observed in various malignant tumors. The aim of this study was to investigate the expression of YAP in non-small cell lung cancer (NSCLC), and to study the possible relationship of YAP expression with the occurrence and development of NSCLC.

Methods: YAP expression was assessed in 40 cases of NSCLC tumor tissues by immunohistochemistry, and their protein and mRNA levels were evaluated through Western blotting and reverse transcription-polymerase chain reaction (PCR), respectively.