Publications by authors named "Jian-Chu Chen"
The integration between RNA-sequencing and micro-spectroscopic techniques has recently profiled the advanced transcriptomic discoveries on the cellular level. In the current study, by combining the sensation approach (including bio-molecules structural evaluation, high throughput next-generation sequencing (HT-NGS), and confocal Raman microscopy) the functionality on the single live cancer cells' ferroptosis and apoptosis signaling pathways is visualized. Our study reveals a hydrophobic tunnel by phycocyanin-isoprene molecule (PC-SIM) electrostatic charge within hepatoma cells (HepG2) that activates the ferritin light chain (FTL) and caspase-8 associate protein (CASP8AP2) ferroptosis responsible genes.
View Article and Find Full Text PDFThe poor biostability and bioavailability of proanthocyanidins limit their application. In this study, it was hypothesized that encapsulation in lecithin-based nanoliposomes using ultrasonic technology improves the above properties. Based on preliminary experiments, the effects of lecithin mass ratio (1-9%, wt.
View Article and Find Full Text PDFArticle Synopsis
- Proanthocyanidins (PAs), found in kiwi leaves, are identified as natural antioxidants, but their specific action pathways were not well-studied before this research.
- The study demonstrated that purified kiwi leaf PAs (PKLPs) effectively lower oxidative damage in Caco-2 cells caused by hydrogen peroxide (H2O2), reducing reactive oxygen species (ROS) and malondialdehyde levels while increasing antioxidant enzyme expression.
- This research highlights the role of PKLPs in activating the Nrf2-ARE signaling pathway, suggesting their potential for applications in promoting cell protection against oxidative stress.
Article Synopsis
- Ultrasound technology (US) is emerging as a key method for extracting proanthocyanidins (PAs) from kiwifruit leaves, enhancing the study of bioactive compounds.
- The study identified optimal conditions for PA extraction using US, which include 40% amplitude, a solvent to solid ratio of 30 mL/g dry weight, at 70°C for 15 minutes.
- Findings showed that the purified kiwifruit leaves PAs (PKLPs) exhibit significant antioxidant activities, suggesting potential applications in drug discovery and food science.
Three cooking methods, namely boiling, steaming, and stir-frying for 5 to 10 min, were used to evaluate the effect on nutrient components, free amino acids, L-ascorbic acid, total phenolic contents, and antioxidant capacities of bamboo shoots (Phyllostachys praecox). Results showed that boiling and stir-frying had a great effect on the nutrient components and they decreased the contents of protein, soluble sugar, and ash, and caused a great loss in the total free amino acids (decreased by 38.35% and 34.
View Article and Find Full Text PDFUltrasound-assisted extraction (UAE) was used to extract phenolic compounds from Satsuma mandarin ( Citrus unshiu Marc.) peels (SMP), and maceration extraction (ME) was used as a control. The effects of ultrasonic time (10, 20, 30, 40, 50, and 60 min), temperature (15, 30, and 40 degrees C), and ultrasonic power (3.
View Article and Find Full Text PDFUltrasound-assisted extraction (UAE) has been widely applied in the extraction of a variety of biologically active compounds including phenolic compounds. However, there is an insufficiency of information on simultaneous extraction of these compounds in this area. In the present study, seven phenolic compounds of two families including cinnamic acids (caffeic, p-coumaric, ferulic, sinapic acid), and benzoic acids (protocatechuic, p-hydroxybenzoic, vanillic acid) from citrus (Citrus unshiuMarc) peels were evaluated by UAE.
View Article and Find Full Text PDFObjective: To probe the safe depth and angle of needling the point Yaotu for treatment of prolapse of intervertebral disc.
Methods: CT technique was used for scanning investigation of the depth and angle of needling the point Yaotu.
Results: When the acupuncture needle was inserted vertically to the external space of intervertebral foramen of human body with arrival of qi, the tip of needle could reach to 73 mm deep, which is the best needling depth and angle.