[Scanning aneugen and clastogen by micronuclei analysis using flow cytometry].

Objective: To explore a flow cytometry (FCM)-based method for discriminating aneugen- or clastogen-induced micronuclei.

Methods: Cells were stained with anti-CD71-FITC and PI, and the PI fluorescent signal intensity of micronucleated reticulocyte (MN-RET) in the peripheral blood of NIH mouse treated with COL or CP was detected by flow cytometry.

Results: The ratio of the median of the intensity of MN-RET fluorescent signals to that of nucleated cell was low in the cyclophosphamide treated mouse, while the median was high in the colchicine treated mouse.

[Detection of genetically modified organisms in food and animal feed by polymerase chain reaction].

Objective: To investigate the presence of genetically modified organisms (GMO) in the foods and animal feed samples in Guangzhou market.

Methods: The presence of GMO were investigated by PCR detection of camv 35S promoter and nos terminator, and the presence of RoundUp Ready Soybean (RRS), Bt176 Maximaizer or Mon810 YieldGard in GMO-positive samples were further determined by PCR detecting their specific DNA fragments respectively.

Results: One corn soup sample, two soybean samples, one potato fries sample as well as two animal feed samples were revealed to be GMO-positive in twenty-two food samples and three animal feed samples, and the presence of RRS in the GMO-positive soybean samples and the two positive animal feed samples were verified by PCR detection of a 129 bp RRS-specific DNA fragment, however, no Bt176 Maximaizer or Mon810 YieldGard specific PCR products were obtained with the GMO-positive corn soup and animal feed DNA samples used as PCR templates.

[Automatic analysis of micronuclei by flow cytometry using anti-CD71-FITC and PI staining].

Objective: To explore flow cytometry (FCM) based method for automatic analysis of micronuclei (MN) in cells staining with anti-CD71-FITC and PI.

Methods: Cell were fixed in -85 degrees C methanol and stained with anti-CD71-FITC and PI, instrument was optimized using malaria-infected erythrocytes as biological standards, a FCM based method for automatic analysis of micronuclei was established. In order to estimate the reliability of the method, the frequencies of micronucleated reticulocytes (MN-RET) in peripheral blood of NIH mice treated with colchicine (COL) was detected using the method.