Genetic alterations and expression of inhibitor of growth 1 in human sporadic colorectal cancer.

Aim: To explore the effect and significance of inhibitor of growth 1 (ING1) gene in carcinogenesis and progression of human sporadic colorectal cancer.

Methods: mRNA expression, mutation, and loss of heterozygosity (LOH) of ING1 gene in 35 specimens of sporadic colorectal cancer tissues and the matched normal mucous membrane tissues were detected by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), PCR-single strain conformation polymorphism (PCR-SSCP) and PCR-simple sequence length polymorphism (PCR-SSLP) using microsatellite markers, respectively.

Results: The average ratios of light intensities of p33(ING1b) and p47(ING1a) mRNA expression in the cancerous tissues were significantly lower than those in normal tissues.

[Mutation, loss of heterozygosity, and expression of tumor suppressor gene ING1 in sporadic colorectal carcinoma].

Background & Objective: Inhibitor of growth 1 (ING(1)) gene has been identified as a novel tumor suppressor gene. Its over-expression can inhibit cell growth, and induce cell apoptosis. This study was to explore the effect and significance of ING(1) gene in occurrence and progression of sporadic colorectal cancer.