Genetic Characteristics of Lipooligosaccharide and Capsular Polysaccharide of from Different Sources in China.

Objective: To determine the distribution of two important virulence factors [lipooligosaccharide (LOS) and capsular polysaccharide (CPS)] in ( ) isolated from different sources in China and to develop a rapid screening method for Guillain-Barré syndrome (GBS)-associated strains.

Methods: Whole-genome sequencing was carried out for 494 strains. The OrthoMCL software was used to define the LOS/CPS gene clusters.

Postoperative short-term use of the nonsteroidal anti-inflammatory drug flurbiprofen did not increase the anastomotic leakage rate in patients undergoing elective gastrointestinal surgery-a retrospective cohort study.

Background: Flurbiprofen has been one of the most commonly used nonsteroidal anti-inflammatory drugs (NSAIDs) in China and other Asian countries for perioperative multimodal analgesia in recent years, yet its association with anastomotic leakage in gastrointestinal anastomoses is unknown. The current study was designed to investigate whether short-term administration of flurbiprofen would increase the risk of anastomotic leakage in patients undergoing gastrointestinal surgery for cancer resection.

Methods: A total of 3653 patients (2487 (66.

3D Biometrics for Hindfoot Alignment Using Weightbearing Computed Tomography.

Background: Weightbearing computed tomography (WBCT) is a useful tool for the assessment of hindfoot alignment (HA). Foot ankle offset (FAO) is a recently introduced parameter, determined from WBCT images using semiautomatic software. The aim of this study was to determine the clinical relevance and reproducibility of FAO for the evaluation of HA.

Rapid Identification and Subtyping of Enterobacter cloacae Clinical Isolates Using Peptide Mass Fingerprinting.

Objective: To establish a domestic database of Enterobacteria cloacae (E. cloacae), and improve the identification efficiency using peptide mass fingerprinting.

Methods: Peptide mass fingerprinting was used for the identification and subtyping of E.

The Role of miRNAs in Cartilage Homeostasis.

Osteoarthritis (OA) is an age-related disease with poorly understood pathogenesis. Recent studies have demonstrated that miRNA might play a key role in OA initiation and development. We reviewed recent publications and elucidated the connection between miRNA and OA cartilage anabolic and catabolic signals, including four signaling pathways: TGF-β/Smads and BMPs signaling, associated with cartilage anabolism; and MAPK and NF-KB signaling, associated with cartilage catabolism.

Identification of Variable-Number Tandem-Repeat (VNTR) Sequences in Acinetobacter pittii and Development of an Optimized Multiple-Locus VNTR Analysis Typing Scheme.

Objective: To develop a multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) assay for Acinetobacter pittii typing.

Methods: Polymorphic VNTRs were searched by Tandem Repeats Finder. The distribution and polymorphism of each VNTR locus were analyzed in all the A.

Identification of early diagnostic antigens from Spirometra erinaceieuropaei sparganum soluble proteins using immunoproteomics.

In order to identify early specific diagnostic antigens of Spirometra erinaceieuropaei (syn. S. erinacei or S.

Antimicrobial susceptibility of Streptococcus sp. to quinupristin-dalfopristin in China.

This study aimed to determine the in vitro activity of quinupristin-alfopristin against Streptococcus sp. isolated in China. This agent is not yet available for clinical use, but it has been tested against a high proportion of resistant Staphylococcus aureus strains.

Rapid and high-throughput identification of recombinant bacteria with mass spectrometry assay.

Objective: To construct a rapid and high-throughput assay for identifying recombinant bacteria based on mass spectrometry.

Methods: Matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) techniques were used to identify 12 recombinant proteins (10 of Yersinia pestis, 1 of Campylobacter jejuni and 1 of Helicobacter pylori). A classification model for the various phase of recombinant bacteria was established, optimized and validated, using MALDI-TOF MS-ClinProTools system.

Serum-free media and the immunoregulatory properties of mesenchymal stem cells in vivo and in vitro.

Background: Mesenchymal stem cells are capable of self-renewal and multi-lineage differentiation. They are used extensively to treat several diseases. Traditionally, mesenchymal stem cells are cultured in serum-containing media, typically supplemented with fetal bovine serum (FBS).

Molecular epidemiological characteristics of Streptococcus pyogenes strains involved in an outbreak of scarlet fever in China, 2011.

Objective: To investigate molecular characterization of streptococcus pyogenes isolates involved in an outbreak of scarlet fever in China in 2011.

Methods: Seventy-four Streptococcal pyogenes involved in an outbreak of scarlet fever were isolated from pediatric patients in the areas with high incidence in China from May to August of 2011. Emm genotyping, pulsed-field gel electrophoresis (PFGE), superantigen (SAg) genes and antimicrobial susceptibility profiling were analyzed for these isolates.

Current undergraduate and postgraduate dental education in China.

China is a developing country with the largest population of any country in the world. The 2010 sixth national census found that China's population was 1,339,724,852, accounting for about 19 percent of the world's total population. However, higher education in the Chinese dental field started late and developed slowly.

In vitro protein expression profile of Campylobacter jejuni strain NCTC11168 by two-dimensional gel electrophoresis and mass spectrometry.

Objective: To investigate the protein expression profiles of the major food-borne pathogen Campylobacter jejuni NCTC11168.

Methods: Membrane and soluble cellular proteins were extracted from the genome-sequenced C. jejuni strain NCTC11168.

Secreted protein HP1286 of Helicobacter pylori strain 26695 induces apoptosis of AGS cells.

Objective: Secreted proteins of Helicobacter pylori (H. pylori) interact with gastric epithelium cells and may contribute to cell damage. Considering the fact that HP0175 and hypothetical conserved protein HP1286 are included in the group and that HP0175 is a well-known apoptosis-induced factor, the present study aims to clarify whether HP1286 plays a role in bacterial pathogenicity or even functions as an apoptosis-induced factor in human stomach.

Case-based learning in clinical courses in a Chinese college of stomatology.

As a medical approach to posing contextualized questions that are based on real-life clinical problems, case-based learning (CBL) is used to stimulate and underpin the acquisition of knowledge, skills, and attitudes through clinical cases. Good effects were acquired in the practice of CBL teaching in English applied by the College of Stomatology, Shanghai Jiao Tong University. Responses from both students and teachers reflected that this course approach won their high acceptance and was worthy of use in selected stomatological courses.

Parallel and partial Fourier imaging with prospective motion correction.

Subject motion during scan is a major source of artifacts in MR examinations. Prospective motion correction is a promising technique that tracks subject motion and adjusts the imaging volume in real time; however, additional retrospective correction may be necessary to achieve robust image quality and compatibility with other imaging options. Real-time realignment of the imaging volume by prospective motion correction changes the coil sensitivity weighting and the field inhomogeneity relative to the imaging volume.

Evaluation of a new real-time PCR assay for detection of Mycoplasma pneumoniae in clinical specimens.

Objective: To establish and evaluate a real-time PCR assay to detect Mycoplasma pneumoniae (M.pneumoniae) in clinical specimens.

Methods: By analysing the whole p1 gene sequence of 60 M.