Publications by authors named "Jian You Lau"
Article Synopsis
- Long noncoding RNAs (lncRNAs) play crucial roles in mammalian cells, but their functional mechanisms and structural organization are not well understood.
- The study focuses on the NORAD lncRNA, which acts as a decoy for Pumilio proteins involved in regulating gene expression and genomic stability, particularly under stress conditions.
- Through analysis, researchers found that NORAD has a modular structure that allows it to interact with different RNAs and cluster binding sites, enhancing its regulatory function, which can inform the design of artificial lncRNAs for specific purposes.
RNA homodimerization is important for various physiological processes, including the assembly of membraneless organelles, RNA subcellular localization, and packaging of viral genomes. However, understanding RNA dimerization has been hampered by the lack of systematic in vivo detection methods. Here, we show that CLASH, PARIS, and other RNA proximity ligation methods detect RNA homodimers transcriptome-wide as "overlapping" chimeric reads that contain more than one copy of the same sequence.
View Article and Find Full Text PDFThe dynamics of SARS-CoV-2 RNA structure and their functional relevance are largely unknown. Here we develop a simplified SPLASH assay and comprehensively map the in vivo RNA-RNA interactome of SARS-CoV-2 genome across viral life cycle. We report canonical and alternative structures including 5'-UTR and 3'-UTR, frameshifting element (FSE) pseudoknot and genome cyclization in both cells and virions.
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