This study employed the methods of serum chemistry, in vivo drug distribution analysis, and network pharmacology to investigate the potential therapeutic effects of Bufei Pills on respiratory disorders and decipher the underlying mechanisms. For the in vivo distribution study, rats were administrated with Bufei Pills, and the absorbed components were analyzed by HPLC-Q-TOF-MS/MS. The results showed that 56 components were present in the rat serum, including neocyclomorusin, asterinin A, neoisostegane, goimisin R, and frehmaglutoside H.
View Article and Find Full Text PDFThe wide dissemination of multidrug-resistant (MDR) Gram-negative bacteria poses a significant global health and security concern. As developing new antibiotics is generally costly, fastidious, and time-consuming, there is an urgent need for alternative therapeutic strategies to address the gap in antibiotic discovery void. This study aimed to investigate the activity of colistin (CS) in combination with a natural product, rutin (RT), to combat against Typhimurium ( Tm) in vitro and in vivo.
View Article and Find Full Text PDFIntroduction: A novel recombinant antigen-based capture enzyme immunoassay (RAg-CEIA) was optimized and used to determine technical parameters for estimating human immunodeficiency virus type 1 (HIV-1) incidence in China.
Methods: We employed orthogonal experimental design to optimize RAg-CEIA by adjusting raw material dilution ratios. The assay was used to measure normalized optical density (ODn) values in 171 longitudinal plasma specimens from 51 HIV-1 seroconverting individuals, plotted against estimated days post-seroconversion.
Aims: Methicillin-resistant Staphylococcus aureus (MRSA) is an important zoonotic pathogen with multidrug resistant phenotypes increasingly prevalent in both human and veterinary clinics. This study evaluated the potential of auranofin (AF) as an antibiotic adjuvant to enhance the anti-MRSA activity of florfenicol (FFC) and established a pharmacokinetic/pharmacodynamic (PK/PD) model to compare the efficacy of FFC alone or in combination with AF against MRSA.
Methods And Results: We observed an increased susceptibility and significant synergistic effects of MRSA to FFC in the presence of AF.
Purpose: Maresin 1 (MaR1) is a specialized pro-resolving mediator with anti-inflammatory properties that promotes tissue repair. This study aims to investigate the molecular involvement of MaR1 in protecting against sepsis-induced acute liver injury (SI-ALI).
Methods: In vivo, a murine SI-ALI model was established using the cecal ligation and puncture (CLP) paradigm, providing a system in which the mechanistic functions of MaR1 could be tested.
As a common malignancy symptom, cancer pain significantly affects patients' quality of life. Approximately 60%-90% of patients with advanced cancer experience debilitating pain. Therefore, a comprehensive treatment system that combines cancer pain suppression and tumor treatment could provide significant benefits for these patients.
View Article and Find Full Text PDFAntibiotic resistance genes (ARGs) are prevalent in raw materials used for composting. The utilization of eco-friendly materials for the removal of ARGs is regarded as an economically effective method. Therefore, this study focused on the impact of incorporating different proportions of vermiculite (0% (CK), 5% (T1), and 10% (T2)) on the dynamics of ARGs during composting.
View Article and Find Full Text PDFBackground: The inactivation of tumor suppressor genes (TSGs) caused by abnormal DNA methylation is confirmed to be widely present in oral potential malignant diseases (OPMDs). Carotenoids like lycopene and astaxanthin can regulate DNA methylation and exert anticancer effects. Therapeutic effect of astaxanthin in OPMDs and oral squamous cell carcinoma (OSCC) models is confirmed, but the relationship between the anti-cancer ability of astaxanthin and its DNA methylation regulation ability remains unclear.
View Article and Find Full Text PDFNitrogen (N) uptake by plant roots from soil is the largest flux within the terrestrial N cycle. Despite its significance, a comprehensive analysis of plant uptake for inorganic and organic N forms across grasslands is lacking. Here we measured in situ plant uptake of 13 inorganic and organic N forms by dominant species along a 3000 km transect spanning temperate and alpine grasslands.
View Article and Find Full Text PDFCisplatin (CDDP) is the primary drug used in the initial treatment of esophageal cancer (EC). However, its side effects and resistance can limit its effectiveness in clinical therapy. Curcumin (Cur)-mediated glutathione (GSH) depletion can reverse resistance, enhance the chemosensitivity of CDDP, and further improve the efficacy of platinum-containing chemotherapy in the treatment of esophageal cancer.
View Article and Find Full Text PDFObjective: This study compared the effects of Flywheel Resistance Squat Training (FRST) vs. Traditional Resistance Squat Training (TRST) on lower body strength in female collegiate basketball players.
Methods: Nineteen participants were randomly assigned to either the FRST group (n = 9) or the TRST group (n = 10) through a random number draw.
A simple strategy for the synthesis of β-keto phosphorodithioates has been developed through the direct oxyphosphorodithiolation of vinyl azides with PS and alcohols in the presence of water. The reaction is conducted at room temperature to provide a number of β-keto phosphorodithioates in moderate to good yields. This methodology has the advantages of simple operation, mild condition, broad substrate scope, and favorable group compatibility.
View Article and Find Full Text PDFExposure to fine particulate matter (PM) is recognized to induce atherosclerosis, but the underlying mechanisms are not fully understood. This study used ambient PM samples collected in one of the highly polluted regions of Guanzhong Plain in China (2017-2020) and an ApoE mouse model to investigate the association between exposure to PM and atherosclerosis. Despite a substantial decrease in the ambient concentration of PM from 266.
View Article and Find Full Text PDFAcute liver failure (ALF) is a serious global disease characterized by rapid onset and high mortality. Currently, the clinical treatment of ALF faces considerable hurdles due to limited medication options and the scarcity of liver transplants. Despite biomacromolecules such as hepatocyte growth factor (HGF) and glutathione (GSH) having been applied for ALF symptom relief in the clinic, they still face substantial challenges including poor stability, difficulty in acting on intracellular targets, and inadequate therapeutic outcome.
View Article and Find Full Text PDFThe discovery of superconductivity in 3d transition-metal compounds with strong magnetism is interesting but rare. Especially for Mn-based compounds, there exist only very limited materials that show superconductivity. Here, the discovery of superconductivity is reported with an onset transition temperature up to 14.
View Article and Find Full Text PDFEthnopharmacological Relevance: Pueraria montana (PM) is a Chinese medicinal herb used to treat alcoholism, inflammation, swelling, and anti-apoptosis. However, the mechanisms and active compounds of PM remain poorly understood.
Aim Of The Study: Chronic inflammatory diseases such as rheumatoid arthritis (RA) can affect multiple joints.
J Exp Clin Cancer Res
November 2024
Background: Triple-negative breast cancer (TNBC) is currently the most aggressive subtype of breast cancer, characterized by high heterogeneity and strong invasiveness, and currently lacks effective therapies. PRMT5, a type II protein arginine methyltransferase, is upregulated in numerous cancers, including TNBC, and plays a critical role, marked it as an attractive therapeutic target. PROTAC (Proteolysis Targeting Chimeras) is an innovative drug development technology that utilizes the ubiquitin-proteasome system (UPS) to degrade target proteins, which is characterized by higher activity, enhanced safety, lower resistance, and reduced toxicity, offering significant value for clinical translation.
View Article and Find Full Text PDFThe origin of hexaploid sweetpotato [Ipomoea batatas (L.) Lam.] remains controversial.
View Article and Find Full Text PDFThis paper establishes a finite element model that includes the interface roughness characteristics to evaluate the stress concentration in the atmospheric plasma sprayed (APS) thermal barrier coatings (TBCs) with an uneven temperature field. We further scrutinize the effects of crack initiation at the interface between the thermally grown oxide (TGO) and the bond coat (BC) and in the ceramic top-coat (TC) on stress redistribution by introducing the debonding model for crack analysis. Results indicate that the interfacial residual stress σ achieves the critical value at the end of the cooling stage.
View Article and Find Full Text PDFHER2-positive gastric cancer has a poor prognosis, with a high incidence of drug resistance and a lack of effective treatments for drug-resistant patients. The exploration of the mechanism of resistance to HER2-targeted therapy in HER2-positive gastric cancer and the identification of effective strategies to reverse it are urgently needed. In this study, we found that HER2-targeted agents upregulated the expression of GSDME and that the overexpression of GSDME attenuated the sensitivity of HER2-targeted agents.
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