Optimizing encephalomyocarditis virus VP1 protein assembly on pseudorabies virus envelope via US9 protein anchoring.

Live herpesvirus-vectored vaccines are critical in veterinary medicine, but they can sometimes offer insufficient protection due to suboptimal antigen expression or localization. Encephalomyocarditis virus (EMCV) is a significant zoonotic threat, with VP1 protein as a key immunogen on its capsid. To enhance immunogenicity, we explored the use of recombinant pseudorabies virus (rPRV) as a vaccine vector against EMCV.

Engineering an enzyme-like catalytic sensor for on-site dual-mode evaluation of total antioxidant capacity.

A novel Fe-MoO nanozyme, engineered with enhanced peroxidase (POD)-like activity through strategic doping and the creation of oxygen vacancies, is introduced to catalyze the oxidation of TMB with high efficiency. Furthermore, Fe-MoO is responsive to single electron transfer (SET) and hydrogen atom transfer (HAT) mechanisms related to antioxidants and can serve as a desirable nanozyme for total antioxidant capacity (TAC) determination. The TAC colorimetric platform can reach a low LOD of 0.

First-in-human evaluation of an independently developed Chinese robot-assisted system for percutaneous coronary intervention.

Background: Several studies have proved the safety and feasibility of robot-assisted percutaneous coronary intervention (PCI) in reducing the occupational hazards of interventionists while achieving precision medicine. However, an independently developed robot-assisted system for PCI in China has not yet emerged. This study aimed to evaluate the safety and feasibility of a robot-assisted system for elective PCI in China.

Minimally invasive thoracoscopic left atrial appendage occlusion compared with transcatheter left atrial appendage closure for stroke prevention in recurrent nonvalvular atrial fibrillation patients after radiofrequency ablation: a prospective cohort study.

Background: Tanscatheter left atrial appendage (LAA) closure and minimally invasive thoracoscopic LAA occlusion are local interventions of LAA for stroke prevention in patients with nonvalvular atrial fibrillation (NVAF). However, the safety and efficacy of these methods have not been compared. This prospective cohort study aimed to assess the safety and efficacy of those two treatment approaches for stroke prevention in NVAF patients.

Palladium-catalyzed coupling reaction of 2-iodobiphenyls with alkenyl bromides for the construction of 9-(diorganomethylidene)fluorenes.

An atom economical protocol for the construction of 9-(diorganomethylidene)fluorenes through palladium-catalyzed coupling reactions of 2-iodobiphenyls with alkenyl bromides has been reported. The reaction proceeds through the C-H activation/oxidative addition/reduction elimination/intramolecular Heck coupling reaction to afford a series of 9-(diorganomethylidene)fluorenes with good yields. Control experiments demonstrate that a five-membered palladacycle acts as a key intermediate and β-H elimination serves as the rate-limiting step.

Hydration and swelling: a theoretical investigation on the cooperativity effect of H-bonding interactions between p-hydroxy hydroxymethyl calix[4]/[5]arene and HO by many-body interaction and density functional reactivity theory.

In order to explore the nature of the hydration and swelling of superabsorbent resin, a theoretical investigation into the cooperativity effect of the H-bonding interactions in the hydrates of four model compounds that can be regarded as the units of hydroquinone formaldehyde resin (HFR) (i.e., O-hydroxymethyl-1,4-dihydroxybenzene, methylene di-O-hydroxymethyl-1,4-dihydroxybenzene, p-hydroxy hydroxymethyl calix[4]arene and p-hydroxy hydroxymethyl calix[5]arene) was carried out by many-body interaction and density functional reactivity theory.

Remnant lipoproteins play an important role of in-stent restenosis in type 2 diabetes undergoing percutaneous coronary intervention: a single-centre observational cohort study.

Background: Increasing evidence has suggested that the presence of remnant lipoproteins is a significant risk factor for atherosclerosis. Remnant lipoproteins are lipoproteins that are rich in triglycerides (TGs), and the main components include very-low-density lipoprotein (VLDL) in the fasting state. Diabetic patients often have hypertriglyceridemia with elevated levels of VLDL cholesterol but normal levels of low-density lipoprotein cholesterol (LDL-C).

Impact of different color fiber sleeves on beam hazards of 532-nm laser and vaporization efficiency.

The 532-nm laser has become increasingly popular for the treatment of urologic diseases. However, laser beam will pose significant hazards for the health of surgeons. In order to reduce beam hazards during surgery, we compared the beam hazards of laser fiber with black sleeves to the traditional fiber with transparent sleeves, and the vaporization efficiency.

Theoretical investigation into the cooperativity effect between the intermolecular π∙π and H-bonding interactions in the curcumin∙cytosine∙HO system.

In order to reveal the mechanism of drug action and design of DNA/RNA-targeted drugs containing aromatic rings, the cooperativity effects between the intermolecular π∙∙∙π and H-bonding interactions in curcumin(drug)∙∙∙cytosine(DNA/RNA base)∙∙∙HO were investigated by the B3LYP-D3 and MP2(full) methods with the 6-311++G(2d,p) basis set. The π∙∙∙π interaction plays an important role in stabilizing the linear ternary complexes with the cooperativity effects, and the cyclic structures suffer the anticooperativity effects. The cooperativity or anticooperativity effects are notable, which could lead to a possible significant change in drug activity.

New predictors of in-stent restenosis in patients with diabetes mellitus undergoing percutaneous coronary intervention with drug-eluting stent.

Background: Percutaneous coronary intervention (PCI) had become the major therapeutic procedure for coronary artery disease (CAD), but the high rate of in-stent restenosis (ISR) still remained an unsolved clinical problem in clinical practice. Increasing evidences suggested that diabetes mellitus (DM) was a major risk factor for ISR, but the risk predictors of ISR in CAD patients with DM had not been well characterized. The aim of this study was to investigate the clinical and angiographic characteristic predictors significantly associated with the occurrence of ISR in diabetic patients following coronary stenting with drug-eluting stent (DES).

Elevated Levels of Very Low-density Lipoprotein Cholesterol Independently Associated with In-stent Restenosis in Diabetic Patients after Drug-eluting Stent Implantation.

Background: High rate of in-stent restenosis (ISR) remained an unsolved clinical problem in clinical practice, especially among patients with diabetes mellitus (DM). Diabetic patients often had hypertriglyceridemia with elevated levels of very low-density lipoprotein cholesterol (VLDL-C). Increasing evidence suggested that VLDL-C was known as a significant risk factor for atherosclerosis and had been recommended as a treatment target by current dyslipidemia guidelines.

Evaluation of the Prostate Imaging Reporting and Data System for Magnetic Resonance Imaging Diagnosis of Prostate Cancer in Patients with Prostate-specific Antigen <20 ng/ml.

Background: The European Society of Urogenital Radiology has built the Prostate Imaging Reporting and Data System (PI-RADS) for standardizing the diagnosis of prostate cancer (PCa). This study evaluated the PI-RADS diagnosis method in patients with prostate-specific antigen (PSA) <20 ng/ml.

Methods: A total of 133 patients with PSA <20 ng/ml were prospectively recruited.

Hydrogen-rich saline injection into the subarachnoid cavity within 2 weeks promotes recovery after acute spinal cord injury.

Hydrogen can relieve tissue-damaging oxidative stress, inflammation and apoptosis. Injection of hydrogen-rich saline is an effective method for transporting molecular hydrogen. We hypothesized that hydrogen-rich saline would promote the repair of spinal cord injury induced by Allen's method in rats.

[Effects of dissolved oxygen in the oxic parts of A/O reactor on degradation of organic pollutants and analysis of microbial community for treating petrochemical wastewater].

Effects of dissolved oxygen (DO) on the biodegradation of organic pollutants were investigated using A/O reactors for the treatment of actual petrochemical wastewater. Two A/O reactors, DO were controlled at 2-3 mg x L(-1) in the oxic parts of reactor A and 5-6 mg x L(-1) of reactor B, were operated in parallel for comparison. The nearly a half of year operation results showed that the effluent COD in reactor A (72.

Nanoscale Fe(0) particles for pentachlorophenol removal from aqueous solution: temperature effect and particles transformation.

Pentachlorophenol (PCP), as an important contaminant which was toxic and intractable, has received extensive attention. In this paper, the temperature effect during the transformation of PCP using nanoscale Fe(0) particles was studied, and the transformation processes of PCP and iron particles was explained. The results revealed that the removal processes of PCP followed pseudo first-order kinetics.

[Reductive degradation of chlorophenols in aqueous solution by gamma irradiation].

Because chlorine is an electron withdrawing group, the highly chlorinated phenols may react quickly with hydrated electrons rather than with hydroxyl radicals. The process of reactions of four chlorophenols (4-CP, 2-CP, 2,4-DCP, 2,4,6-TCP) with e(aq)(-) was investigated in aqueous solutions by detecting the concentration of CPs, Cl- and intermediates. In the e(aq)(-) reductive system, the experimental results showed that the order of four kinds of chlorophenol degradation and dechlorination was 2,4,6-TCP > 2,4-DCP > 2-CP > 4-CP.

A comparative theoretical investigation into the strength of the trigger-bond in the Na⁺, Mg²⁺ and HF complexes involving the nitro group of R-NO₂ (R = -CH₃, -NH₂ and -OCH₃) or the C = C bond of (E)-O₂N-CH = CH-NO₂.

A comparative theoretical investigation into the change in strength of the trigger-bond upon formation of the Na(+), Mg(2+) and HF complexes involving the nitro group of RNO₂ (R = -CH₃, -NH₂, -OCH₃) or the C = C bond of (E)-O₂N-CH = CH-NO₂ was carried out using the B3LYP and MP2(full) methods with the 6-311++G**, 6-311++G(2df,2p) and aug-cc-pVTZ basis sets. Except for the Mg(2+)⋯π system with (E)-O2N-CH = CH-NO₂ (i.e.

Remote ischemic preconditioning reduces myocardial injury in patients undergoing coronary stent implantation.

Background: Myocardial necrosis occurs frequently in elective percutaneous coronary intervention (PCI) and is associated with subsequent major adverse cardiovascular events (MACEs). This study assessed the protective effect of remote ischemic preconditioning (RIPC) in patients undergoing successful drug-eluting stent implantation with normal baseline troponin values.

Methods: We analyzed 205 participants with normal baseline troponin values undergoing successful coronary stent implantation.

Theoretical investigation on the structure and thermodynamic properties of the 2,4-dinitroimidazole complex with methanol.

The structure and thermodynamic properties of the 2, 4-dinitroimidazole complex with methanol were investigated using the B3LYP and MP2(full) methods with the 6-31++G(2d,p) and 6-311++G(3df,2p) basis sets. Four types of hydrogen bonds [N-H⋯O, C-H⋯O, O-H⋯O (nitro oxygen) and O-H⋯π] were found. The hydrogen-bonded complex having the highest binding energy had a N-H⋯O hydrogen bond.

[Bioaugmented removal of pyridine and the microbial community dynamic analysis].

The bioaugmented removal of pyridine was investigated through introducing immobilized Paracoccus sp. strain KT-5 capable of degrading pyridine into the lab-scale sequencing batch reactor (SBR) inoculated with activated sludge. The terminal restriction fragment length polymorphisms (T-RFLP) was used to analyzed the microbial community dynamics of two reactors during the whole operation process.

N-Methyl-4-nitro-anilinium chloride.

The asymmetric unit of the title salt, C(7)H(9)N(2)O(2) (+)·Cl(-), contains two independent cations and anions. In the crystal, each N-methyl-4-nitro-anilinium cation is linked to two Cl(-) anions via N-H⋯Cl hydrogen bonds. π-π stacking is observed between the benzene rings of adjacent cations [centroid-to-centroid distances = 3.

Safety and feasibility of repeated percutaneous transradial coronary intervention in the same route.

Background: The radial approach has been increasingly used as an alternative to femoral access. And more procedures using repeated transradial coronary intervention (r-TRI) are performed. However, few data about r-TRI has been obtained.