The ICF2 gene Zbtb24 specifically regulates the differentiation of B1 cells via promoting heme synthesis.

Background: Loss-of-function mutations of ZBTB24 cause immunodeficiency, centromeric instability, and facial anomalies syndrome 2 (ICF2). ICF2 is a rare autosomal recessive disorder with immunological defects in serum antibodies and circulating memory B cells, resulting in recurrent and sometimes fatal respiratory and gastrointestinal infections. The genotype-phenotype correlation in patients with ICF2 indicates an essential role of ZBTB24 in the terminal differentiation of B cells.

Revisiting the antigen markers of vector-borne parasitic diseases identified by immunomics: identification and application to disease control.

Introduction: Protein microarray is a promising immunomic approach for identifying biomarkers. Based on our previous study that reviewed parasite antigens and recent parasitic omics research, this article expands to include information on vector-borne parasitic diseases (VBPDs), namely, malaria, schistosomiasis, leishmaniasis, babesiosis, trypanosomiasis, lymphatic filariasis, and onchocerciasis.

Areas Covered: We revisit and systematically summarize antigen markers of vector-borne parasites identified by the immunomic approach and discuss the latest advances in identifying antigens for the rational development of diagnostics and vaccines.

Special prognostic phenomenon for patients with mid-range ejection fraction heart failure: a systematic review and meta-analysis.

Background: Clinical features and outcomes of heart failure (HF) with mid-range ejection fraction (HFmrEF) remain controversial. Thus, we systematically reviewed literatures of clinical research to assess and analyze characteristics and prognosis of patients with HFmrEF.

Methods: PubMed, Embase, and Web of Science were searched for cohort studies up to April 23, 2019.

[Transpiration of Brassica chinensis L. in a plastic greenhouse covered with insect-proof nets in lower reaches of Yangtze River: a simulation study].

With the climate data inside and outside a plastic greenhouse as driving variables, and the greenhouse structure, insect-proof net material, and characteristic breadth and leaf area index of Brassica chinensis L. as parameters; a canopy transpiration model for greenhouse B. chinensis was established, based on Penmam-Monteith transpiration model.

[Simulation model on the formation of greenhouse sweet pepper leaf area index].

Leaf area index (LAI) is one of the most important crop parameters in photosynthesis-driving crop growth simulation model and canopy evapotranspiration simulation model, while air temperature and radiation are the important climate factors affecting crop leaf growth. In this paper, experiments with different sweet pepper (Capsicum annuum L.) cultivars and sowing dates were conducted in greenhouse to quantitatively analyze the relationships of the number of unfolding leaves per plant, the number of old leaves removed per plant, and the length of each leaf with air temperature and radiation.

[Effects of stem numbers per ground area on the quality of standard cut Chrysanthemum morifolium in greenhouse: simulation model].

In order to understand the effects of stem numbers per ground area on the quality of standard cut Chrysanthemum morifolium, an experiment with different cultivars, different stem numbers per plant, different planting densities, and different planting dates was conducted in a greenhouse in Shanghai in 2005 and 2006. The effects of stem numbers per ground area on the canopy leaf area index and external quality of standard cut C. morifolium were quantified using the experimental data.

[Effects of transplanting date and density on appearance quality of greenhouse single-flower cut chrysanthemum].

Transplanting date and density are the important factors affecting the appearance quality of chrysanthemum. The study on the greenhouse single-flower cut chrysanthemum (Chrysanthemum morifolium cv. Shenma) showed that within the ranges of test transplanting date and density, the plant height and neck length increased, while the leaf number per plant, stem diameter, plant fresh mass and flower diameter decreased with the delay of transplanting date and the increase of transplanting density.

Inhibitory effect of emodin on tissue inhibitor of metalloproteinases-1 (TIMP-1) expression in rat hepatic stellate cells.

Emodin inhibited expression of both transforming growth factor beta1 (TGFbeta1)- and phorbol ester (PMA)-induced tissue inhibitors of metalloproteinase-1 (TIMP-1) in an immortalized rat hepatic stellate cell line, HSC-T6, by Western blot and reverse transcription polymerase chain reaction. Reporter gene assays showed that emodin reduced both basal and PMA-induced activated protein-1 (AP-1) promoter activities. Electrophoretic mobility shift assay revealed that emodin reduced AP-1 DNA binding activities in HSC-T6 cells.

[Tumor relevance analysis of a highly conserved gene by using gene microarray hybridization].

Preliminary function research of a highly conserved human gene,which was cloned from human fetal cDNA library during large-scale cDNA sequencing,is illustrated in this article. Bioinformatics analysis indicates that this gene is highly conserved in human, mouse, fruit fly, thaliana and fission yeast. Other bioinformatics analysis implies its relevance with tumors.

Cloning and characterization of a novel human RNA binding protein gene PNO1.

Present work reported the cloning and characterization of a human novel RNA binding gene Partner of NOB1 (PNO1), with a length of 1637bp and a putative open reading frame of 759 bp, isolated from human kidney. It is composed of seven exons and is localized on chromosome 2p14. Western blot showed that the molecular weight of PNO1 is about 35kDa.