Background: Minimal change disease (MCD) is a common pathological type of nephrotic syndrome in children and adults, and the mechanisms remain obscure. The diagnosis of MCD still relies on renal biopsy, lacking effective biological markers. This study explores the diagnostic value of circulating anti-nephrin antibody in MCD patients and evaluates the correlation with disease activity indicators such as proteinuria.
View Article and Find Full Text PDFGlutaredoxin (Grx) is a small molecular protein widely found in both prokaryotes and eukaryotes, serving various biological functions, including participation in redox reactions and exerting anti-apoptotic effects[1]. To evaluate the protective effect of recombinant Grx1 against oxidative stress, we constructed the pET-30a (+)/Grx1 recombinant plasmid and performed soluble expression and purification of the recombinant Grx1. In vitro experiments, including ABTS and DPPH radical scavenging assays, showed that recombinant Grx1 has significant antioxidant activity.
View Article and Find Full Text PDFBackground: , which are filamentous cyanobacteria, have gained significant popularity in the food industry, medicine, and aquaculture.
Methods: In this study, our objective was to explore the influence of on the gut microbiota and exercise capacity of mice undergoing high-intensity exercise. Twenty-four male BALB/c mice were divided into four groups, with six mice in each group.
We performed all-atom and coarse-grained simulations of lipid bilayer mixtures of the unsaturated lipid DOPC, with saturated lipids having the same 18-carbon acyl tails and different headgroups, to understand their mechanical properties. The secondary lipids were DSPG, DSPA, DSPS, DSPC, and DSPE. The DOPC:DSPG system with 65:35 molar ratio was the softest, with area compressibility modulus ∼ 22% smaller than the pure DOPC value.
View Article and Find Full Text PDFThe severe contamination of the plasticizer dibutyl phthalate (DBP) in agriculture soils is often accompanied by a decrease in nutrient utilization. Though the combined application of a variety of microorganisms can simultaneously address the problems of soil contamination and nutrient deprivation, the activity and function of microorganisms can be severely inhibited by DBP, and studies on their protection under DBP contamination are almost non-existent. In this study, a compound bacterial agent KPSB was prepared by optimising with FeO-modified biochar loaded with DBP-degrading bacterium Enterobacterium sp.
View Article and Find Full Text PDFThe Fc receptor (FcRn) inhibitors can ameliorate autoimmune conditions such as myasthenia gravis through a rapid and specific clearance of serum IgG levels, and they also have potential for future use in a wider variety of antibody-mediated autoimmune diseases. Some patients with therapy-refractory autoimmune encephalitis (AE) continue to be unresponsive to initial and secondary treatment regimens. A 32-year-old male presented with predominant psychiatric symptoms and seizures, along with imaging evidence indicating multifocal cerebral cortical involvement.
View Article and Find Full Text PDFAs a representative agent of bicyclic antidepressants, venlafaxine (VEN) has become widely used worldwide and is frequently detected in surface waters with concentrations ranging from ng/L to µg/L. To evaluate the toxicological effects of such medications on aquatic species, studies on environmentally relevant concentrations are essential. Zebrafish were used as a model organism to assess growth and development in larvae and examine tissue accumulation, oxidative stress, and DNA methylation in adults.
View Article and Find Full Text PDFGlomerular endothelial cells (GECs) are pivotal in developing glomerular sclerosis disorders. The advancement of focal segmental glomerulosclerosis (FSGS) is intimately tied to disruptions in lipid metabolism. Sphingosine-1-phosphate (S1P), a molecule transported by high-density lipoproteins (HDL), exhibits protective effects on vascular endothelial cells by upregulating phosphorylated endothelial nitric oxide synthase (p-eNOS) and enhancing nitric oxide (NO) production.
View Article and Find Full Text PDFβ-Adrenergic signaling activates cAMP-dependent PKA, which regulates the activity of L-type voltage-gated calcium channels such as Ca1.2. Several PKA target sites in the C-terminal tail of Ca1.
View Article and Find Full Text PDFFunctional fibers, retaining nanoscale characteristics or nanomaterial properties, represent a significant advance in nanotechnology. Notably, the combination of scalable manufacturing with cutting-edge nanotechnology further expands their utility across numerous disciplines. Manufacturing kilometer-scale functional fibers with nanoscale properties are critical to the evolution of smart textiles, wearable electronics, and beyond.
View Article and Find Full Text PDFPurpose: To explore the value of tissue quantitative diffusion analysis of ultrasound elastography in the diagnosis of early-stage chronic kidney disease (CKD).
Methods: The observation group comprised 54 patients with early-stage CKD treated at Fuzhou No 7 Hospital, and the control group consisted of 40 healthy individuals who underwent physical examinations at the same hospital. The renal parenchyma of the participants were examined using ultrasonography, color Doppler ultrasonography, and tissue quantitative diffusion analysis of ultrasound elastography.
Methylene blue (MB) is a refractory organic pollutant that poses a potential threat to the aquatic environment. Fenton reaction is considered a primrose strategy to treat MB. However, the traditional Fenton process is plagued by narrow pH application range, poor stability, and secondary pollution.
View Article and Find Full Text PDFObjective: Schizophrenia (SCZ) is a globally prevalent, severe chronic mental disorder, with cognitive dysfunction being one of its core symptoms. Notably, overweight is exceedingly common among individuals with SCZ, and overweight can also impact cognitive function. Therefore, the relationship between overweight and cognition in SCZ is a clinical issue that is in need of research attention.
View Article and Find Full Text PDFThe fascinating role of SPRR3 in various malignant tumors has prompted extensive research to unravel its expression patterns and prognostic significance. To comprehensively investigate SPRR3, we leveraged multiple datasets containing invaluable biomedical information, specifically focusing on the comparative analysis of SPRR3 gene expression levels across different cancer types. Meticulous examination of lung adenocarcinoma allowed us to delve deeper into the correlation between SPRR3 expression and its molecular biological functions.
View Article and Find Full Text PDFThe grouting repair process of the sealing baffle on the surface of dam cracks can improve the repair slurry diffusion distance in the cracks; however, the process must be optimized. To address the problem of crack repair technology of underwater dam concrete with one section open and the other closed, a grouting analysis model was established. By adjusting various parameters in the grouting process, the influences of the grouting pressure, position of the grouting hole, diameter of the grouting hole, and water pressure on the diffusion of the Bingham fluid repair slurry in these cracks were studied.
View Article and Find Full Text PDFEggshell color plays important biological roles and attracts the attention of both egg retailers and researchers. However, whether non-coding RNAs are involved in pigment deposition among different eggshell colors remains unknown. In this study, RNA sequencing was used to analyse the uterine gland transcriptome (CircRNA and miRNA) of Changshun chicken blue-shell hens producing four different eggshell color eggs including dark blue PK(DB) and light blue (LB), dark brown and greenish (between blue and pink, DP) and pink (p).
View Article and Find Full Text PDFConventional computing systems based on von Neumann architecture face challenges such as high power consumption and limited data processing capability. Improving device performance scaling guided by Moore's Law becomes increasingly difficult. Emerging memristors can provide a promising solution for achieving high-performance computing systems with low power consumption.
View Article and Find Full Text PDFBackground: Infectious bone defect refers to severe bone tissue damage caused by skeletal infection, often resulting in impaired skeletal function and intense inflammatory responses. Treating infectious bone defects is a challenging task, as conventional treatment methods often fail to completely eliminate the infection focus and may easily lead to inflammatory responses in the bone defect area.
Objective: To examine the impacts of bone transport (BT) in conjunction with drug-loaded calcium sulfate (DLCS) on the expression of inflammatory factors and vascular endothelial growth factor (VEGF) in rats with infectious bone defects.
Idiopathic nephrotic syndrome (NS) is a heterogeneous group of glomerular disorders which includes two major phenotypes: minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS). MCD and FSGS are classic types of primary podocytopathies. We aimed to explore the molecular mechanisms in NS triggered by primary podocytopathies and evaluate diagnostic value of the selected proteomic signatures by analyzing blood proteome profiling.
View Article and Find Full Text PDFβ-barrel membrane proteins populate the outer membrane of Gram-negative bacteria, mitochondria, and chloroplasts, playing significant roles in multiple key cellular pathways. Characterizing the functions of these membrane proteins in vivo is often challenging due to the complex protein network in the periplasm of Gram-negative bacteria (or intermembrane space in mitochondria and chloroplasts) and the presence of other outer membrane proteins. In vitro reconstitution into lipid-bilayer-like environments such as nanodiscs or proteoliposomes provides an excellent method for examining the specific function and mechanism of these membrane proteins in an isolated system.
View Article and Find Full Text PDFObjective: To explore the clinical effect of predeposit, salvage, and hemodilution autotransfusion on patients with femoral shaft fracture (FSF) surgery.
Methods: Selected patients with FSF were randomly divided into three groups: intraoperative blood salvage autotransfusion, preoperative hemodilution autohemotransfusion, and predeposit autotransfusion. Five days after the operation, the body temperature, heart rate, blood platelet (PLT), and hemoglobin (Hb) of patients were determined.
Sialylated immunoglobulin G (IgG) is a vital glycoprotein in breast milk with the ability to promote the growth of in gut microbiota and relieve inflammatory bowel disease (IBD) symptoms . Here, it was found that the microcapsules with sialylated IgG could protect and release sialylated IgG with its structure and function in the intestine. Furthermore, the sialylated IgG microcapsules alleviated the clinical symptoms (body weight, feed quantity, and colon length loss), decreased disease activity index score, suppressed the production of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β, IFN-γ, and MCP-1) and endotoxin (lipopolysaccharide), and enhanced the intestinal mucosal barrier (Claudin1, Muc2, Occludin, and ZO-1) in dextran sulfate sodium (DSS)-induced colitis mice.
View Article and Find Full Text PDFEnvironmental awareness is receiving increasing attention in the petroleum industry, especially when associated with chemical agents applied in enhanced oil recovery (EOR) technology. The bio-based surfactant sodium cocoyl alaninate (SCA) is environmentally friendly and can be easily biodegraded, which makes it a promising alternative to traditional surfactants. Herein, the SCA surfactant is proposed as a foaming agent for enhanced oil recovery.
View Article and Find Full Text PDFAll animals must maintain genome and proteome integrity, especially when experiencing endogenous or exogenous stress. To cope, organisms have evolved sophisticated and conserved response systems: unfolded protein responses (UPRs) ensure proteostasis, while DNA damage responses (DDRs) maintain genome integrity. Emerging evidence suggests that UPRs and DDRs crosstalk, but this remains poorly understood.
View Article and Find Full Text PDFDeep learning has great potential on decoding EEG in brain-computer interface. While common deep learning algorithms cannot directly train models with data from multiple individuals because of the inter-individual differences in EEG. Collecting enough data for each subject to satisfy the training of deep learning would result in an increase in training cost.
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