Mapping multi-regional functional connectivity of astrocyte-neuronal networks during behaviors.

Significance: Diverse behaviors rely on coordinated activity and multi-regional functional connectivity within astrocyte-neuronal networks. However, current techniques for simultaneously measuring astrocytic and neuronal activities across multiple brain regions during behaviors remain limited.

Aim: We propose a multi-fiber solution that can simultaneously record activities of astrocyte-neuronal networks across multiple regions during behaviors.

Intelligent alert system for predicting invasive mechanical ventilation needs via noninvasive parameters: employing an integrated machine learning method with integration of multicenter databases.

The use of invasive mechanical ventilation (IMV) is crucial in rescuing patients with respiratory dysfunction. Accurately predicting the demand for IMV is vital for clinical decision-making. However, current techniques are invasive and challenging to implement in pre-hospital and emergency rescue settings.

DNA-functionalized MOF fluorescent probes for the enzyme-free and pretreatment-free detection of MicroRNA in serum.

MicroRNA (miRNA) is a promising biomarker that plays an important role in various biomedical applications, especially in cancer diagnosis. However, the current miRNA detection technology has inherent limitations such as complex operation, expensive testing cost and excessive detection time. In this study, a dual signal amplification biosensor based on DNA-functionalized metal-organic frameworks (MOFs) fluorescent probes, MFPBiosensor, was established for the enzyme-free and pretreatment-free detection of the colon cancer (CC) marker miR-23a.

Development of double-layer poly (amino acid) modified electrochemical sensor for sensitive and direct detection of betamethasone in cosmetics.

Screening for illegal use of glucocorticoids (GCs) in cosmetics by electrochemical methods is extremely challenging due to the poor electrochemical activity of GCs. In this study, poly-L-Serine/poly-Taurine modified electrode (P(Tau)/P(L-Ser)/GCE) was prepared for sensitive and direct determination of betamethasone in cosmetics by a simple two-step in situ electropolymerization reaction. The relevant parameters of preparation and electroanalytical conditions were respectively studied, including the concentration of polymerization solution, the number of scanning circles and the scanning rate.

The identification of blood pressure variation with hypovolemia based on the volume compensation method.

The purpose of this study is to identify the blood pressure variation, which is important in continuous blood pressure monitoring, especially in the case of low blood volume, which is critical for survival. A pilot study was conducted to identify blood pressure variation with hypovolemia using five Landrace pigs. New multi-dimensional morphological features of Photoplethysmography (PPG) were proposed based on experimental study of hemorrhagic shock in pigs, which were strongly correlated with blood pressure changes.

A machine learning method for predicting the probability of MODS using only non-invasive parameters.

Objectives: Timely and accurate prediction of multiple organ dysfunction syndrome (MODS) is essential for the rescue and treatment of trauma patients However, existing methods are invasive, easily affected by artifacts and can be difficult to perform in a pre-hospital setting. We aim to develop prediction models for patients with MODS using only non-invasive parameters.

Method: In this study, records from 2319 patients were extracted from the Multiparameter Intelligent Monitoring in Intensive Care Ⅲ database (MIMIC Ⅲ), based on the sequential organ failure assessment (SOFA) score.

Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine.

Silica nanoparticles hold tremendous potential for the encapsulation of enzymes. However, aqueous alcohol solutions and catalysts are prerequisites for the production of silica nanoparticles, which are too harsh for maintaining the enzyme activity. Herein, a procedure without any organic solvents and catalysts (acidic or alkaline) is developed for the synthesis of silica-encapsulated glucose-oxidase-coated magnetic nanoparticles by a facile self-assembly route, avoiding damage of the enzyme structure in the reaction system.

Two-photon and Three-photon Fluorescence of Triton X-100 in the Ultraviolet Region.

We report, to our best knowledge, the first observation of two-photon and three-photon fluorescence of Triton X-100 (TX-100) in water and cyclohexane. The observed multiphoton fluorescence (MF) falls in the ultraviolet region 280-340nm as its one photon fluorescence does. Effects of excitation wavelengths and solution concentrations on the fluorescence spectra are investigated.

A Novel Nanomedicine Ameliorates Acute Inflammatory Bowel Disease by Regulating Macrophages and T-Cells.

polysaccharide (RMP), one of the most important active components of , has been attracting increasing interest because of its potent bioactive properties, including anti-inflammatory, antitumor, and antidiabetic effects. Despite the great therapeutic potential of RMP, its inherent properties of low bioavailability and brief biological half-life have limited its applications to the clinic. Thus, RMP was packaged by poly(lactic--glycolic acid) (PLGA) nanoparticles to develop a novel anti-inflammatory nanomedicine (PLGA-RMP) in this study.

Prognostic Signature for Lung Adenocarcinoma Patients Based on Cell-Cycle-Related Genes.

Objective: To screen lung adenocarcinoma (LUAC)-specific cell-cycle-related genes (CCRGs) and develop a prognostic signature for patients with LUAC.

Methods: The GSE68465, GSE42127, and GSE30219 data sets were downloaded from the GEO database. Single-sample gene set enrichment analysis was used to calculate the cell cycle enrichment of each sample in GSE68465 to identify CCRGs in LUAC.

SIZ1 negatively regulates aluminum resistance by mediating the STOP1-ALMT1 pathway in Arabidopsis.

Sensitive to proton rhizotoxicity 1 (STOP1) functions as a crucial regulator of root growth during aluminum (Al) stress. However, how this transcription factor is regulated by Al stress to affect downstream genes expression is not well understood. To explore the underlying mechanisms of the function and regulation of STOP1, we employed a yeast two hybrid screen to identify STOP1-interacting proteins.

Two-Photon Fluorescence Study of Olive Oils at Different Excitation Wavelengths.

Two-photon fluorescence (TPF) of olive oils is discovered and observed experimentally for the first time. Variations of the single-photon fluorescence (SPF) and TPF with the excitation wavelength are investigated for four different olive oils. The results show that fluorescence of the cosmetic olive oils (COO) is very weak and exhibits only one spectral peak around 490 nm.

Exciting Wavelength and Concentration Related Two-Photon Fluorescence of Single and Mixed Laser Dyes.

Two-photon nonlinear process induced fluorescence of Rhodamine 6G (R6G), Rhodamine B (RB), and their mixed aqueous solutions in mass proportion of 1:1, is experimentally observed by different exciting wavelengths. It shows that, for each sample, the exciting wavelength can influence the fluorescence intensity considerably but only slightly influence the peak wavelength of the spectrum. The optimal exciting wavelengths of R6G and the mixed dyes are around 700 nm.

A machine learning approach for mortality prediction only using non-invasive parameters.

At present, the traditional scoring methods generally utilize laboratory measurements to predict mortality. It results in difficulties of early mortality prediction in the rural areas lack of professional laboratorians and medical laboratory equipment. To improve the efficiency, accuracy, and applicability of mortality prediction in the remote areas, a novel mortality prediction method based on machine learning algorithms is proposed, which only uses non-invasive parameters readily available from ordinary monitors and manual measurement.

AtHB7/12 Regulate Root Growth in Response to Aluminum Stress.

Aluminum (Al) stress is a major limiting factor for plant growth and crop production in acid soils. At present, only a few transcription factors involved in the regulation of Al resistance have been characterized. Here, we used reversed genetic approach through phenotype analysis of overexpressors and mutants to demonstrate that AtHB7 and AtHB12, two HD-Zip I transcription factors, participate in Al resistance.

A new method for identifying the acute respiratory distress syndrome disease based on noninvasive physiological parameters.

Early diagnosis and prevention play a crucial role in the treatment of patients with ARDS. The definition of ARDS requires an arterial blood gas to define the ratio of partial pressure of arterial oxygen to fraction of inspired oxygen (PaO2/FiO2 ratio). However, many patients with ARDS do not have a blood gas measured, which may result in under-diagnosis of the condition.

A matter of time: grappling with everyday ethical tensions at the confluence between policy and practice in a psychiatric unit.

Objective: To provide insights on emergent ethical tensions experienced by mental health practitioners during system re-organisation, which is sufficiently grounded in empirical data at the local level to inform policy on recovery at institutional and provincial levels.

Method: Ethnographic methods using narrative and critical phenomenological resources over 24 months.

Findings: Everyday ethical tensions emerged at the confluence of different experiences of time, for example, how a context of increasing pressure to decrease patients' length of stay at the hospital () challenged efforts to listen to and advocate for what mattered to patients () and maintain the integrity of interventions ().

Dye-enhanced third upconversion Raman overtone in the ultraviolet region under intense excitation of a 532 nm laser.

We report, to our best knowledge, the first observation of enhanced third upconversion Raman overtone of water in the ultraviolet region 240-380 nm by Rhodamine 6G (R6G) under intense excitation of a 532 nm laser. Its spectral peak is mostly around 288 nm. This ultraviolet spectrum has also been obtained for purified water on the Raman spectrometer LRS-3 and fluorescence spectrometer RF-5301PC, which have photomultiplier tubes.

Inference of time-delayed gene regulatory networks based on dynamic Bayesian network hybrid learning method.

Gene regulatory networks (GRNs) research reveals complex life phenomena from the perspective of gene interaction, which is an important research field in systems biology. Traditional Bayesian networks have a high computational complexity, and the network structure scoring model has a single feature. Information-based approaches cannot identify the direction of regulation.

Characterization of VuMATE1 Expression in Response to Iron Nutrition and Aluminum Stress Reveals Adaptation of Rice Bean (Vigna umbellata) to Acid Soils through Cis Regulation.

Rice bean (Vigna umbellata) VuMATE1 appears to be constitutively expressed at vascular system but root apex, and Al stress extends its expression to root apex. Whether VuMATE1 participates in both Al tolerance and Fe nutrition, and how VuMATE1 expression is regulated is of great interest. In this study, the role of VuMATE1 in Fe nutrition was characterized through in planta complementation assays.

Physiological and Molecular Analysis of Aluminium-Induced Organic Acid Anion Secretion from Grain Amaranth (Amaranthus hypochondriacus L.) Roots.

Grain amaranth (Amaranthus hypochondriacus L.) is abundant in oxalate and can secrete oxalate under aluminium (Al) stress. However, the features of Al-induced secretion of organic acid anions (OA) and potential genes responsible for OA secretion are poorly understood.

Resting state glutamate predicts elevated pre-stimulus alpha during self-relatedness: A combined EEG-MRS study on "rest-self overlap".

Recent studies have demonstrated neural overlap between resting state activity and self-referential processing. This "rest-self" overlap occurs especially in anterior cortical midline structures like the perigenual anterior cingulate cortex (PACC). However, the exact neurotemporal and biochemical mechanisms remain to be identified.

A comparison of neural responses to appetitive and aversive stimuli in humans and other mammals.

Distinguishing potentially harmful or beneficial stimuli is necessary for the self-preservation and well-being of all organisms. This assessment requires the ongoing valuation of environmental stimuli. Despite much work on the processing of aversive- and appetitive-related brain signals, it is not clear to what degree these two processes interact across the brain.

Sustained neurochemical plasticity in central terminals of mouse DRG neurons following colitis.

Sensitization of dorsal root ganglia (DRG) neurons is an important mechanism underlying the expression of chronic abdominal pain caused by intestinal inflammation. Most studies have focused on changes in the peripheral terminals of DRG neurons in the inflamed intestine but recent evidence suggests that the sprouting of central nerve terminals in the dorsal horn is also important. Therefore, we examine the time course and reversibility of changes in the distribution of immunoreactivity for substance P (SP), a marker of the central terminals of DRG neurons, in the spinal cord during and following dextran sulphate sodium (DSS)-induced colitis in mice.