The involvement of multiple ABC transporters in daunorubicin efflux in Streptomyces coeruleorubidus.

Streptomyces genus produces a large number of antibiotics, which are always synthesized by specific biosynthetic gene clusters (BGCs). To resist autotoxicity, transporters encoded by genes located within BGC occasionally pump antibiotic along with transporter encoded by gene located outside BGC. Daunorubicin is an anthracycline antibiotic biosynthesized by Streptomyces species, playing a crucial role in the treatment of leukaemia.

[Characteristics of Soil Erosion and Nitrogen Loss in Vegetable Field Under Natural Rainfall].

The effects of vegetable planting on soil loss and nutrient loss, runoff, soil erosion, and nitrogen (ammonium nitrogen and nitrate nitrogen) losses under individual rainfalls of fruit- and leaf-vegetable fields between April to October in 2021 were observed using in-situ observation testing. The results showed that: ① the runoff, erosion, and nitrogen loss of the fruit-vegetable pattern (eggplant-chili) were 1.27-2.

Bioremediation of mercury-polluted soil and water by the plant symbiotic fungus .

Fungi are central to every terrestrial and many aquatic ecosystems, but the mechanisms underlying fungal tolerance to mercury, a global pollutant, remain unknown. Here, we show that the plant symbiotic fungus degrades methylmercury and reduces divalent mercury, decreasing mercury accumulation in plants and greatly increasing their growth in contaminated soils. does this by demethylating methylmercury via a methylmercury demethylase (MMD) and using a mercury ion reductase (MIR) to reduce divalent mercury to volatile elemental mercury.

A Combinational Strategy for Effective Heterologous Production of Functional Human Lysozyme in .

Human lysozyme (hLYZ), known for its bacteriolytic activity, is widely applied in the food and pharmaceutical industries as an antimicrobial agent. However, its extensive application was limited by its low large-scale production efficiency. In this study, a combinational method of integrating codon optimization, multiple gene copies, and ER molecular chaperone co-expression was developed to improve the heterologous production of hLYZ in GS115.

Palatal Radicular Groove Morphology of the Maxillary Incisors: A Case Series Report.

Introduction: Teeth with a palatal radicular groove are challenging to diagnose, treat, and save. We classified this condition using cone-beam computed tomographic (CBCT) cross-sectional configurations combined with radiographic and morphologic observations during an intentional replantation procedure to identify and analyze the characteristics of palatal radicular grooves comprehensively as a reference for diagnosis, treatment planning, and prognosis evaluation of this condition.

Methods: Eight cases with palatal radicular grooves present in permanent maxillary incisors were investigated by radiography and CBCT imaging before intentional replantation.