Genome-wide characterization of pepper DREB family members and biological function of CaDREB32 in response to salt and osmotic stresses.

Dehydration response element binding (DREB) transcription factors play multiple roles in plant growth, development and response to abiotic stress. However, their biological functions in response to salt and osmotic stress in vegetables of the Solanaceae family are largely unclear. Here, 49 CaDREB genes classified into six groups were identified in the pepper genome.

Long-term phosphate exposure effects on juvenile turbot (Scophthalmus maximus): Growth, metabolism, and adaptive responses.

Phosphate (PO₄-P) is one of the main pollutants contributing to water eutrophication in natural ecosystems and is also a neglected factor in water management. The toxicological response mechanisms of aquatic organisms to phosphate remain unclear. In the current study, juvenile Scophthalmus maximus were exposed to 0 (CK), 60 (LP), and 120 mg/L (HP) of PO₄-P for 60 days.

Interactively Fusing Global and Local Features for Benign and Malignant Classification of Breast Ultrasound Images.

Objective: Breast ultrasound (BUS) is used to classify benign and malignant breast tumors, and its automatic classification can reduce subjectivity. However, current convolutional neural networks (CNNs) face challenges in capturing global features, while vision transformer (ViT) networks have limitations in effectively extracting local features. Therefore, this study aimed to develop a deep learning method that enables the interaction and updating of intermediate features between CNN and ViT to achieve high-accuracy BUS image classification.

A fly in the ointment: the re-discovered holotype of 'Trypeta' cylindrica Walker, 1853 belongs to the genus Coelopacidia Enderlein (Diptera: Tephritidae), not Chyliza Fallén (Psilidae).

The 'type' of the fly species 'Trypeta' cylindrica Walker, 1853, from the William Saunders Collection, was misidentified and subsequently placed in the genus Chyliza Fallén (Psilidae). A specimen from the same collection with identical label data but belonging to Coelopacidia Enderlein (Tephritidae) is hereby identified as the correct holotype and the species should therefore be referred to as Coelopacidia cylindrica (Walker, 1853). Chyliza pallidipes Lamb, 1917 stat.

An artificial intelligence-assisted digital microfluidic system for multistate droplet control.

Digital microfluidics (DMF) is a versatile technique for parallel and field-programmable control of individual droplets. Given the high level of variability in droplet manipulation, it is essential to establish self-adaptive and intelligent control methods for DMF systems that are informed by the transient state of droplets and their interactions. However, most related studies focus on droplet localization and shape recognition.

Mutual learning with memory for semi-supervised pest detection.

Effectively monitoring pest-infested areas by computer vision is essential in precision agriculture in order to minimize yield losses and create early scientific preventative solutions. However, the scale variation, complex background, and dense distribution of pests bring challenges to accurate detection when utilizing vision technology. Simultaneously, supervised learning-based object detection heavily depends on abundant labeled data, which poses practical difficulties.

It's time to reevaluate the list of priority polycyclic aromatic compounds: Evidence from a large urban shallow lake.

Monitoring only 16 priority PAHs (Pri-PAHs) may greatly underestimate the pollutant load and toxicity of polycyclic aromatic compounds (PACs) in aquatic environments. There is an urgent need to reevaluate the list of priority PACs. To determine which PACs deserve priority monitoring, the occurrence, sources, and toxicity of 78 PACs, including 24 parent PAHs (Par-PAHs), 49 alkylated PAHs (Alk-PAHs), 3 oxygenated PAHs (OPAHs), carbazole, and dibenzothiophene were investigated for the first time in Lake Chaohu sediments, China.

Genome-wide characterization of Remorin gene family and their responsive expression to abiotic stresses and plant hormone in Brassica napus.

Remorin proteins could be positively related to salt and osmotic stress resistance in rapeseed. Remorins (REMs) play a crucial role in adaptations to adverse environments. However, their roles in abiotic stress and phytohormone responses in oil crops are still largely unknown.

Myricetin Acts as an Inhibitor of Type II NADH Dehydrogenase from .

Background: is a common pathogenic microorganism in humans and animals. Type II NADH oxidoreductase (NDH-2) is the only NADH:quinone oxidoreductase present in this organism and represents a promising target for the development of anti-staphylococcal drugs. Recently, myricetin, a natural flavonoid from vegetables and fruits, was found to be a potential inhibitor of NDH-2 of .

Sea Anemone-Inspired Conducting Polymer Sensing Platform for Integrated Detection of Tumor Protein Marker and Circulating Tumor Cell.

Combining the detection of tumor protein markers with the capture of circulating tumor cells (CTCs) represents an ultra-promising approach for early tumor detection. However, current methodologies have not yet achieved the necessary low detection limits and efficient capture. Here, a novel polypyrrole nanotentacles sensing platform featuring anemone-like structures capable of simultaneously detecting protein biomarkers and capturing CTCs is introduced.

The novel fish miRNA, Soc-miR-118, functions as a negative regulator in NF-κB-mediated inflammation by targeting IL-6 in teleost fish.

Inflammation is initiated as a protective response of the organism to remove invading bacterial and initiate the healing process. Prolonged inflammation and excessive production of inflammatory cytokines lead to inflammatory disorders or autoimmune diseases. Thus, different layers of negative regulators are needed to achieve balances between protective immunity and inflammatory pathology.

Zinc caproate: Ecofriendly synthesis, structural characterization, and antibacterial action.

Disease-causing microorganisms such as Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) are among the primary contributors to morbidity and mortality of diarrhea in humans.

Taxonomic review of the Strongylophthalmyia ustulata group (Diptera: Brachycera: Strongylophthalmyiidae) from China, with the descriptions of three new species.

The Strongylophthalmyia ustulata group (Diptera: Brachycera: Strongylophthalmyiidae) from China is revised. Five species are recognized, including three new species (S. elongata sp.

Genome-edited rabbits: Unleashing the potential of a promising experimental animal model across diverse diseases.

Animal models are extensively used in all aspects of biomedical research, with substantial contributions to our understanding of diseases, the development of pharmaceuticals, and the exploration of gene functions. The field of genome modification in rabbits has progressed slowly. However, recent advancements, particularly in CRISPR/Cas9-related technologies, have catalyzed the successful development of various genome-edited rabbit models to mimic diverse diseases, including cardiovascular disorders, immunodeficiencies, aging-related ailments, neurological diseases, and ophthalmic pathologies.

Comparative transcriptome analysis reveals potential regulatory mechanisms of genes and immune pathways following Vibrio harveyi infection in red drum (Sciaenops ocellatus).

Red drum (Sciaenops ocellatus), as an important economical marine fish, has been affected by various bacterial diseases in recent years. Vibrio harveyi cause fatal vibriosis in S. ocellatus, leading to massive mortality and causing significant setbacks in aquaculture.

Potential role of N6-methyladenosine modification in the development of Parkinson's disease.

N6-methyladenosine (m6A) represents the most abundant modification of messenger RNA (mRNA) and is regulated by methyltransferases (writers), demethylases (erasers), and m6A-binding proteins (readers). A dynamic modification process is implicated in nearly every critical stage of RNA metabolism, including mRNA stability, transcription, translation, splicing, nuclear export, and decay. Notably, m6A methylation is significantly enriched in the brain and has recently been shown to be associated with neurodevelopmental disorders and the development of Parkinson's disease (PD).

A review of the subgenus Meigen, 1803 (Diptera, Brachycera, Psilidae) in China.

The subgenus Loxocera ( Meigen, 1803) (Diptera, Brachycera, Psilidae) in China is reviewed. Six species are recognized, including four new species: L. (L.

High Strength MXene/PBONF Heterogeneous Membrane with Excellent Ion Selectivity for Efficient Osmotic Energy Conversion.

Layered MXene nanofluidic membranes still face the problems of low mechanical property, poor ion selectivity, and low output power density. In this work, we successfully constructed heterostructured membranes with the combination of the layered channels of the MXene layer on the top and the nanoscale poly(-phenylene-benzodioxazole) nanofiber (PBONF) layer on the bottom through a stepwise filtration method. The as-prepared MXene/PBONF-50 heterogeneous membrane exhibits high mechanical properties (strength of 221.

Comparative transcriptome analysis reveals growth and molecular pathway of body color regulation in turbot (Scophthalmus maximus) exposed to different light spectrum.

Fish body color changes play vital roles in adapting to ecological light environment and influencing market value. However, the initial mechanisms governing the changes remain unknown. Here, we scrutinized the impact of light spectrum on turbot (Scophthalmus maximus) body coloration, exposing them to red, blue, and full light spectra from embryo to 90 days post hatch.

Engineered domain-inlaid Nme2Cas9 adenine base editors with increased on-target DNA editing and targeting scope.

Background: Nme2ABE8e has been constructed and characterized as a compact, accurate adenine base editor with a less restrictive dinucleotide protospacer-adjacent motif (PAM: N4CC) but low editing efficiency at challenging loci in human cells. Here, we engineered a subset of domain-inlaid Nme2Cas9 base editors to bring the deaminase domain closer to the nontarget strand to improve editing efficiency.

Results: Our results demonstrated that Nme2ABE8e-797 with adenine deaminase inserted between amino acids 797 and 798 has a significantly increased editing efficiency with a wide editing window ranging from 4 to 18 bases in mammalian cells, especially at the sites that were difficult to edit by Nme2ABE8e.

Retroperitoneoscopic Clampless, Sutureless Hybrid Therapy in the Management of Renal Hilar Tumors.

Objective: We aimed to investigate the outcomes and feasibility of a retroperitoneoscopic clampless, sutureless hybrid technique in the management of renal hilar tumors.

Methods: A retrospective cohort of consecutive patients with renal hilar tumors who received retroperitoneoscopic clampless, sutureless hybrid therapy between January 2017 and April 2021 was included. The hybrid surgical technique involved microwave ablation (MWA), followed by clampless tumor enucleation and sutureless hemostasis.

A Floating Capsule Electrochemical System for In Situ and Multichannel Ion-Selective Sensing.

Free-floating electrochemical sensors are promising for in situ bioprocess monitoring with the advantages of movability, a lowered risk of contamination, and a simplified structure of the bioreactor. Although floating sensors were developed for the measurement of physical and chemical indicators such as temperature, velocity of flow, pH, and dissolved oxygen, it is the lack of available electrochemical sensors for the determination of the inorganic ions in bioreactors that has a significant influence on cell culture. In this study, a capsule-shaped electrochemical system (iCapsuleEC) is developed to monitor ions including K, NH, Na, Ca, and Mg based on solid-contact ion-selective electrodes (SC-ISEs).

A deep supervised transformer U-shaped full-resolution residual network for the segmentation of breast ultrasound image.

Purpose: Breast ultrasound (BUS) is an important breast imaging tool. Automatic BUS image segmentation can measure the breast tumor size objectively and reduce doctors' workload. In this article, we proposed a deep supervised transformer U-shaped full-resolution residual network (DSTransUFRRN) to segment BUS images.

First principles insights into the interaction mechanism of iron doped thermally activated kaolinite with Cd and Pb pollutants in organic solid waste incineration flue gas.

Incineration of organic solid wastes is accompanied by the heavy metal emission through flue gas. As an inexpensive and efficient heavy metal adsorbent, the improvement of kaolinite adsorption performance for heavy metals has drawn widespread interests. In this work, the interaction mechanisms between various kaolinite surfaces and Cd/Pb species are explored through first principles calculations.

A review of the subgroup (Diptera, Brachycera, Strongylophthalmyiidae) from China, with the descriptions of three new species.

The species belonging to the subgroup of the group (Diptera: Brachycera: Strongylophthalmyiidae) from China are reviewed. Six species are recognized, including three new species: , , and Evenhuis, 2020 and Shatalkin, 1981 are recorded from China for the first time, and is also recorded from South Korea for the first time. An identification key to the Asian species of the subgroup is provided.