Simultaneous bioimaging recognition of Al and Cu in living-cell, and further detection of F and S by a simple fluorogenic benzimidazole-based chemosensor.

A simple Schiff base (BMSA) prepared from salicylaldehyde and 2-(1H-benzo[d]imidazol-2-yl)aniline was evaluated as an efficient fluorescent chemosensor for the selective recognition of Aland Cu over other common metal ions. This sensor could detect Al3 in CHOH/PBS with distinct emission red-shift (the detection limit 0.31μM)and Cuin CHOH/Tris-HCL (the detection limit 0.

Proteolysis targeting peptide (PROTAP) strategy for protein ubiquitination and degradation.

Ubiquitination proteasome pathway (UPP) is the most important and selective way to degrade proteins in vivo. Here, a novel proteolysis targeting peptide (PROTAP) strategy, composed of a target protein binding peptide, a linker and a ubiquitin E3 ligase recognition peptide, was designed to recruit both target protein and E3 ligase and then induce polyubiquitination and degradation of the target protein through UPP. In our study, the PROTAP strategy was proved to be a general method with high specificity using Bcl-xL protein as model target in vitro and in cells, which indicates that the strategy has great potential for in vivo application.