A rapid and sensitive enrichment technique, field enhanced sample injection-dynamic pH junction-sweeping (FESI-DypH-sweeping) was successfully developed for the simultaneous separation and concentration of alkaloids and stereoisomers of Uncariae ramulus cum uncis (UR) by cyclodextrin electrokinetic chromatography (CDEKC) with diode array detection system. The sample was prepared in a low-conductivity (FESI), low-pH (DypH) sample matrix (4 mM phosphate buffer, 3% methanol, pH=3), and the background electrolyte (BGE) consisted of a high-conductivity, high-pH buffer (40 mM phosphate buffer, pH 7.0, 8 mg/mL carboxymethyl-β-cyclodextrin, and 30% methanol).
View Article and Find Full Text PDFIntroduction: Yangxinshi tablet (YXST) is a effective traditional Chinese medicine in treating cardiovascular diseases such as heart failure and myocardial infarction.
Objectives: This study aims to develop a method for screening thrombin inhibitors from YXST using an online immobilized enzyme microreactor (IMER) based on capillary electrophoresis (CE).
Materials And Methods: Thrombin (THR) was immobilized on the capillary's inner wall using polydopamine (PDA).
Introduction: Toddalia asiatica (TA) is a classical traditional Chinese medicine used to treat rheumatoid arthritis and contusions. However, research regarding TA quality control is currently limited.
Objective: We aimed to establish a strategy for identifying quality markers that can be used for the evaluation of the quality of TA.
A comprehensive strategy for effective identification of total constituents in Chinese patent medicine has been advanced applying full scan-preferred parent ions capture-static and active exclusion (FS-PIC-SAE) acquisition coupled with intelligent deep-learning supported mass defect filter (MDF) process, with Naoxintong capsule (NXT) as a case. Online comprehensive two-dimensional liquid chromatography (2DLC) coupled with Q-TOF-MS/MS system was established for obtaining the excellent separation and detection performance of total components, which could exhibit excellent peak capacity with 1052 and orthogonality with 0.69.
View Article and Find Full Text PDFA simple and efficient vortex-assisted matrix solid phase dispersion with a ultra-high-performance liquid chromatography-triple quadrupole mass spectrometer (VA-MSPD-UHPLC-MS/MS) was applied for simultaneous extraction and determination of seven alkaloids and three organic acids from . The optimal extraction conditions of the target components were obtained by Box-Behnken design (BBD) combined with response surface methodology (RSM). The results of the method validation showed that this analytical method displayed good linearity with a correlation coefficient (r) no lower than 0.
View Article and Find Full Text PDFAn efficient and green ultrasonic-assisted micellar extraction method coupled with ultra-high performance liquid chromatography with photodiode array detection (UHPLC-PDA) was developed for the multi-ingredients quantitative analysis of Yangxinshi Tablet (YXST). The active ingredients were extracted from YXST using trehalose lipid biosurfactant solution as an environmentally friendly extraction solution. The response surface methodology (RSM) based on Box-Behnken design (BBD) was utilized to seek for the optimum extraction conditions of target analytes.
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