Specific Monitoring the DNA Helicase Function via Anchor-Embedded DNA Probe.

DNA helicases play a pivotal role in maintaining genome integrity by unwinding the DNA double helix and are often considered promising targets for drug development. However, assessing specific DNA helicase activity in living cells remains challenging. Herein, the first anchor-embedded duplex (ATED) probe, 17GC, is constructed to uniquely monitor the unwinding activity of Werner syndrome helicase (WRN), a clinical anticancer target.

An off-on electrochemiluminescence detection for microRNAs based on TiO nanotubes sensitized with gold nanoparticles as enhanced emitters.

A sensitive electrochemiluminescence (ECL) assay for microRNAs (miRNAs) based on a semiconductor nanomaterial sensitized with noble-metal Au nanoparticles (NPs) is successfully developed. TiO nanotubes (NTs) were equipped with Au NPs to obtain an enhanced ECL emitter. Then, an ECL assay for miRNA-21 was fabricated, which was based on the use of probe 2 DNA-functionalized Pt/PAMAM nanocomposites (NCs) assembled on the surface of Au/TiO NT conjugate via DNA hybridization between probe 1 DNA and capture DNA.

TiO nanotubes loaded with CdS nanocrystals as enhanced emitters of electrochemiluminescence: application to an assay for prostate-specific antigen.

An enhanced cathodic electrochemiluminescence (ECL) assay for prostate-specific antigen (PSA) is developed based on the in situ activation of a semiconductor nanomaterial. An excellent ECL emitter (CdS/TiO nanotubes) was fabricated by the combination of TiO nanotubes (NTs) and thioglycolic acid-capped CdS nanocrystals (NCs). After the activation of the hydrogen peroxide-citric acid solution, the ECL signal was enhanced 265 times compared with that of the original TiO NT with HO as co-reactant.

The effects of molecular collisions between the mobile phase and the solute in gas-solid chromatography.

In chromatographic processes, molecular collisions between the mobile phase and the solute result in the transfer of kinetic energy. Based on these interactions, the relationship between the gauge pressure of the carrier gas at the column inlet and the partition frequency of the solute is derived; consequently, the relationship between the column temperature and partition frequency can be obtained. These relationships have been experimentally validated.

Measurement and calculation of surface tension of molten Sn-Bi alloy.

The surface tension of molten Sn-Bi (mole fraction X(Bi) = 0.455) alloy has been determined by the sessile drop method at oxygen partial pressure (P(O2)) of 1.0x10(-6) MPa and different temperatures.